Restriction map for
pFN21AE6067
(Linear) (Six-base) MAPSORT of: KIEE6067.seq Check: 9942 from: 1 to: 963
KIEE6067.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 812 963
Size: 780 32 151
Fragments arranged by size:
780 151 32
AflIII A'CryG_T
Cuts at: 0 745 963
Size: 745 218
AleI CACnn'nnGTG
Cuts at: 0 766 963
Size: 766 197
AlwNI CAG_nnn'CTG
Cuts at: 0 31 963
Size: 31 932
BanI G'GyrC_C
Cuts at: 0 651 963
Size: 651 312
BanII G_rGCy'C
Cuts at: 0 185 963
Size: 185 778
BbsI GAAGACnn'nnnn_
Cuts at: 0 654 786 963
Size: 654 132 177
Fragments arranged by size:
654 177 132
BbvCI CC'TCA_GC
Cuts at: 0 154 719 963
Size: 154 565 244
Fragments arranged by size:
565 244 154
BclI T'GATC_A
Cuts at: 0 866 963
Size: 866 97
BglI GCCn_nnn'nGGC
Cuts at: 0 643 963
Size: 643 320
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 963
Size: 79 884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 963
Size: 579 384
Bpu10I CC'TnA_GC
Cuts at: 0 154 719 963
Size: 154 565 244
Fragments arranged by size:
565 244 154
BsaHI Gr'CG_yC
Cuts at: 0 311 963
Size: 311 652
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 109 963
Size: 106 3 854
Fragments arranged by size:
854 106 3
BseYI C'CCAG_C
Cuts at: 0 31 323 349 810 963
Size: 31 292 26 461 153
Fragments arranged by size:
461 292 153 31 26
BsiHKAI G_wGCw'C
Cuts at: 0 116 963
Size: 116 847
BsmBI CGTCTCn'nnnn_
Cuts at: 0 283 963
Size: 283 680
Bsp1286I G_dGCh'C
Cuts at: 0 116 185 963
Size: 116 69 778
Fragments arranged by size:
778 116 69
BspHI T'CATG_A
Cuts at: 0 857 963
Size: 857 106
BsrBI CCG'CTC
Cuts at: 0 713 963
Size: 713 250
BsrDI GCAATG_nn'
Cuts at: 0 272 963
Size: 272 691
BtgI C'CryG_G
Cuts at: 0 377 963
Size: 377 586
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 513 548 963
Size: 513 35 415
Fragments arranged by size:
513 415 35
DraIII CAC_nnn'GTG
Cuts at: 0 507 963
Size: 507 456
EaeI y'GGCC_r
Cuts at: 0 398 509 963
Size: 398 111 454
Fragments arranged by size:
454 398 111
EarI CTCTTCn'nnn_
Cuts at: 0 83 334 583 963
Size: 83 251 249 380
Fragments arranged by size:
380 251 249 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 780 812 963
Size: 579 201 32 151
Fragments arranged by size:
579 201 151 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 753 963
Size: 753 210
HaeII r_GCGC'y
Cuts at: 0 926 963
Size: 926 37
MfeI C'AATT_G
Cuts at: 0 140 963
Size: 140 823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 963
Size: 219 744
MscI TGG'CCA
Cuts at: 0 400 511 963
Size: 400 111 452
Fragments arranged by size:
452 400 111
MslI CAynn'nnrTG
Cuts at: 0 430 646 670 766 862 963
Size: 430 216 24 96 96 101
Fragments arranged by size:
430 216 101 96 96 24
MspA1I CmG'CkG
Cuts at: 0 49 963
Size: 49 914
NcoI C'CATG_G
Cuts at: 0 377 963
Size: 377 586
NspI r_CATG'y
Cuts at: 0 749 963
Size: 749 214
PciI A'CATG_T
Cuts at: 0 745 963
Size: 745 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 31 345 507 963
Size: 31 314 162 456
Fragments arranged by size:
456 314 162 31
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 798 830 963
Size: 798 32 133
Fragments arranged by size:
798 133 32
PshAI GACnn'nnGTC
Cuts at: 0 790 963
Size: 790 173
SapI GCTCTTCn'nnn_
Cuts at: 0 334 583 963
Size: 334 249 380
Fragments arranged by size:
380 334 249
SfcI C'TryA_G
Cuts at: 0 420 894 963
Size: 420 474 69
Fragments arranged by size:
474 420 69
StuI AGG'CCT
Cuts at: 0 736 963
Size: 736 227
StyI C'CwwG_G
Cuts at: 0 377 656 963
Size: 377 279 307
Fragments arranged by size:
377 307 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 404 616 963
Size: 404 212 347
Fragments arranged by size:
404 347 212
TatI w'GTAC_w
Cuts at: 0 203 963
Size: 203 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 563 595 963
Size: 563 32 368
Fragments arranged by size:
563 368 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 346 963
Size: 346 617
XmnI GAAnn'nnTTC
Cuts at: 0 736 963
Size: 736 227
Enzymes that do cut:
AcuI AflIII AleI AlwNI BanI BanII BbsI BbvCI
BclI BglI BmrI BpmI Bpu10I BsaHI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BtgI CspCI
DraIII EaeI EarI Eco57MI FalI HaeII MfeI MmeI
MscI MslI MspA1I NcoI NspI PciI PflMI PpiI
PshAI SapI SfcI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI
BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BspEI BspMI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI