Restriction map for
pFN21AE6064
(Linear) (Six-base) MAPSORT of: KIEE6064.seq Check: 4171 from: 1 to: 960
KIEE6064.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AccI GT'mk_AC
Cuts at: 0 113 960
Size: 113 847
AgeI A'CCGG_T
Cuts at: 0 730 960
Size: 730 230
AleI CACnn'nnGTG
Cuts at: 0 751 960
Size: 751 209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 960
Size: 166 794
ApoI r'AATT_y
Cuts at: 0 43 960
Size: 43 917
AvaI C'yCGr_G
Cuts at: 0 20 275 960
Size: 20 255 685
Fragments arranged by size:
685 255 20
Bme1580I G_kGCm'C
Cuts at: 0 306 960
Size: 306 654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 786 945 960
Size: 292 494 159 15
Fragments arranged by size:
494 292 159 15
Bpu10I CC'TnA_GC
Cuts at: 0 508 960
Size: 508 452
BsaAI yAC'GTr
Cuts at: 0 381 960
Size: 381 579
BsaWI w'CCGG_w
Cuts at: 0 730 960
Size: 730 230
BseYI C'CCAG_C
Cuts at: 0 71 129 444 676 796 960
Size: 71 58 315 232 120 164
Fragments arranged by size:
315 232 164 120 71 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 960
Size: 597 363
BsiEI CG_ry'CG
Cuts at: 0 704 960
Size: 704 256
BsmBI CGTCTCn'nnnn_
Cuts at: 0 866 960
Size: 866 94
Bsp1286I G_dGCh'C
Cuts at: 0 306 960
Size: 306 654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 86 109 272 316 797 960
Size: 86 23 163 44 481 163
Fragments arranged by size:
481 163 163 86 44 23
BsrBI CCG'CTC
Cuts at: 0 521 960
Size: 521 439
BsrFI r'CCGG_y
Cuts at: 0 730 960
Size: 730 230
BssSI C'ACGA_G
Cuts at: 0 573 960
Size: 573 387
BstAPI GCAn_nnn'nTGC
Cuts at: 0 475 960
Size: 475 485
Bsu36I CC'TnA_GG
Cuts at: 0 913 960
Size: 913 47
BtgI C'CryG_G
Cuts at: 0 26 362 638 752 960
Size: 26 336 276 114 208
Fragments arranged by size:
336 276 208 114 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 657 960
Size: 657 303
BtsI GCAGTG_nn'
Cuts at: 0 476 559 585 960
Size: 476 83 26 375
Fragments arranged by size:
476 375 83 26
EaeI y'GGCC_r
Cuts at: 0 200 383 566 755 960
Size: 200 183 183 189 205
Fragments arranged by size:
205 200 189 183 183
EarI CTCTTCn'nnn_
Cuts at: 0 97 613 960
Size: 97 516 347
Fragments arranged by size:
516 347 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 225 493 960
Size: 225 268 467
Fragments arranged by size:
467 268 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 786 945 960
Size: 292 494 159 15
Fragments arranged by size:
494 292 159 15
EcoO109I rG'GnC_Cy
Cuts at: 0 75 909 953 960
Size: 75 834 44 7
Fragments arranged by size:
834 75 44 7
EcoRI G'AATT_C
Cuts at: 0 43 960
Size: 43 917
EcoRV GAT'ATC
Cuts at: 0 552 960
Size: 552 408
HaeII r_GCGC'y
Cuts at: 0 151 960
Size: 151 809
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 269 301 960
Size: 269 32 659
Fragments arranged by size:
659 269 32
MscI TGG'CCA
Cuts at: 0 202 385 568 757 960
Size: 202 183 183 189 203
Fragments arranged by size:
203 202 189 183 183
MslI CAynn'nnrTG
Cuts at: 0 142 184 751 781 960
Size: 142 42 567 30 179
Fragments arranged by size:
567 179 142 42 30
MspA1I CmG'CkG
Cuts at: 0 28 960
Size: 28 932
NcoI C'CATG_G
Cuts at: 0 362 960
Size: 362 598
NspI r_CATG'y
Cuts at: 0 696 960
Size: 696 264
PfoI T'CCnGG_A
Cuts at: 0 523 960
Size: 523 437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 590 622 960
Size: 590 32 338
Fragments arranged by size:
590 338 32
PpuMI rG'GwC_Cy
Cuts at: 0 75 953 960
Size: 75 878 7
Fragments arranged by size:
878 75 7
PshAI GACnn'nnGTC
Cuts at: 0 587 960
Size: 587 373
SacII CC_GC'GG
Cuts at: 0 29 960
Size: 29 931
SanDI GG'GwC_CC
Cuts at: 0 953 960
Size: 953 7
SexAI A'CCwGG_T
Cuts at: 0 116 960
Size: 116 844
SfcI C'TryA_G
Cuts at: 0 879 960
Size: 879 81
SgrAI Cr'CCGG_yG
Cuts at: 0 730 960
Size: 730 230
SmaI CCC'GGG
Cuts at: 0 22 960
Size: 22 938
StuI AGG'CCT
Cuts at: 0 458 960
Size: 458 502
StyI C'CwwG_G
Cuts at: 0 362 960
Size: 362 598
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 690 960
Size: 451 239 270
Fragments arranged by size:
451 270 239
TatI w'GTAC_w
Cuts at: 0 188 960
Size: 188 772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 810 960
Size: 255 555 150
Fragments arranged by size:
555 255 150
XmaI C'CCGG_G
Cuts at: 0 20 960
Size: 20 940
XmnI GAAnn'nnTTC
Cuts at: 0 602 960
Size: 602 358
Enzymes that do cut:
AccI AgeI AleI AlfI ApoI AvaI Bme1580I BpmI
Bpu10I BsaAI BsaWI BseYI BsgI BsiEI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
HaeII Hin4I MscI MslI MspA1I NcoI NspI PfoI
PpiI PpuMI PshAI SacII SanDI SexAI SfcI SgrAI
SmaI StuI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfiI SfoI SgfI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XhoI ZraI