Restriction map for
pFN21AE6062
(Linear) (Six-base) MAPSORT of: KIEE6062.seq Check: 7677 from: 1 to: 960
KIEE6062.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 792 885 960
Size: 792 93 75
Fragments arranged by size:
792 93 75
AhdI GACnn_n'nnGTC
Cuts at: 0 823 960
Size: 823 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 508 960
Size: 508 452
ApoI r'AATT_y
Cuts at: 0 107 960
Size: 107 853
BamHI G'GATC_C
Cuts at: 0 71 960
Size: 71 889
BanII G_rGCy'C
Cuts at: 0 291 960
Size: 291 669
BbsI GAAGACnn'nnnn_
Cuts at: 0 178 913 960
Size: 178 735 47
Fragments arranged by size:
735 178 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 498 960
Size: 464 34 462
Fragments arranged by size:
464 462 34
BclI T'GATC_A
Cuts at: 0 20 960
Size: 20 940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 960
Size: 103 857
Bpu10I CC'TnA_GC
Cuts at: 0 34 960
Size: 34 926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 960
Size: 432 528
BseYI C'CCAG_C
Cuts at: 0 578 960
Size: 578 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 960
Size: 863 97
Bsp1286I G_dGCh'C
Cuts at: 0 291 960
Size: 291 669
BspHI T'CATG_A
Cuts at: 0 189 635 960
Size: 189 446 325
Fragments arranged by size:
446 325 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 792 885 960
Size: 792 93 75
Fragments arranged by size:
792 93 75
BsrDI GCAATG_nn'
Cuts at: 0 662 960
Size: 662 298
BstBI TT'CG_AA
Cuts at: 0 474 960
Size: 474 486
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 814 960
Size: 369 445 146
Fragments arranged by size:
445 369 146
BstYI r'GATC_y
Cuts at: 0 71 960
Size: 71 889
BtgI C'CryG_G
Cuts at: 0 374 890 960
Size: 374 516 70
Fragments arranged by size:
516 374 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 793 960
Size: 793 167
EaeI y'GGCC_r
Cuts at: 0 221 371 960
Size: 221 150 589
Fragments arranged by size:
589 221 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 960
Size: 103 857
EcoO109I rG'GnC_Cy
Cuts at: 0 52 960
Size: 52 908
EcoRI G'AATT_C
Cuts at: 0 107 960
Size: 107 853
EcoRV GAT'ATC
Cuts at: 0 597 960
Size: 597 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 809 841 960
Size: 809 32 119
Fragments arranged by size:
809 119 32
HindIII A'AGCT_T
Cuts at: 0 477 960
Size: 477 483
MscI TGG'CCA
Cuts at: 0 223 373 960
Size: 223 150 587
Fragments arranged by size:
587 223 150
MslI CAynn'nnrTG
Cuts at: 0 172 367 569 758 769 960
Size: 172 195 202 189 11 191
Fragments arranged by size:
202 195 191 189 172 11
MspA1I CmG'CkG
Cuts at: 0 250 960
Size: 250 710
NcoI C'CATG_G
Cuts at: 0 374 890 960
Size: 374 516 70
Fragments arranged by size:
516 374 70
NruI TCG'CGA
Cuts at: 0 293 960
Size: 293 667
NspI r_CATG'y
Cuts at: 0 518 960
Size: 518 442
PfoI T'CCnGG_A
Cuts at: 0 48 350 960
Size: 48 302 610
Fragments arranged by size:
610 302 48
PpuMI rG'GwC_Cy
Cuts at: 0 52 960
Size: 52 908
PstI C_TGCA'G
Cuts at: 0 532 960
Size: 532 428
PvuII CAG'CTG
Cuts at: 0 250 960
Size: 250 710
SfcI C'TryA_G
Cuts at: 0 528 960
Size: 528 432
SmlI C'TyrA_G
Cuts at: 0 411 960
Size: 411 549
StuI AGG'CCT
Cuts at: 0 80 960
Size: 80 880
StyI C'CwwG_G
Cuts at: 0 374 890 960
Size: 374 516 70
Fragments arranged by size:
516 374 70
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 100 960
Size: 100 860
Enzymes that do cut:
AarI AhdI AlfI ApoI BamHI BanII BbsI BcgI
BclI BpmI Bpu10I BpuEI BseYI BsgI Bsp1286I BspHI
BspMI BsrDI BstBI BstXI BstYI BtgI BtgZI EaeI
Eco57MI EcoO109I EcoRI EcoRV Hin4I HindIII MscI MslI
MspA1I NcoI NruI NspI PfoI PpuMI PstI PvuII
SfcI SmlI StuI StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI