Restriction map for
pFN21AE6057
(Linear) (Six-base) MAPSORT of: KIEE6057.seq Check: 6159 from: 1 to: 960
KIEE6057.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 742 960
Size: 742 218
AflIII A'CryG_T
Cuts at: 0 337 960
Size: 337 623
AlwNI CAG_nnn'CTG
Cuts at: 0 458 960
Size: 458 502
BanI G'GyrC_C
Cuts at: 0 760 960
Size: 760 200
BanII G_rGCy'C
Cuts at: 0 173 960
Size: 173 787
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 960
Size: 36 924
BbvCI CC'TCA_GC
Cuts at: 0 289 960
Size: 289 671
BclI T'GATC_A
Cuts at: 0 203 552 960
Size: 203 349 408
Fragments arranged by size:
408 349 203
BglI GCCn_nnn'nGGC
Cuts at: 0 163 793 802 960
Size: 163 630 9 158
Fragments arranged by size:
630 163 158 9
Bme1580I G_kGCm'C
Cuts at: 0 763 960
Size: 763 197
Bpu10I CC'TnA_GC
Cuts at: 0 289 960
Size: 289 671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 960
Size: 917 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 70 960
Size: 70 890
BsaAI yAC'GTr
Cuts at: 0 792 960
Size: 792 168
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 463 960
Size: 100 363 497
Fragments arranged by size:
497 363 100
BseYI C'CCAG_C
Cuts at: 0 172 486 960
Size: 172 314 474
Fragments arranged by size:
474 314 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 960
Size: 388 572
Bsp1286I G_dGCh'C
Cuts at: 0 173 763 960
Size: 173 590 197
Fragments arranged by size:
590 197 173
BspHI T'CATG_A
Cuts at: 0 419 960
Size: 419 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 742 818 960
Size: 742 76 142
Fragments arranged by size:
742 142 76
BsrDI GCAATG_nn'
Cuts at: 0 524 843 960
Size: 524 319 117
Fragments arranged by size:
524 319 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 454 960
Size: 454 506
BtgI C'CryG_G
Cuts at: 0 158 623 960
Size: 158 465 337
Fragments arranged by size:
465 337 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 603 960
Size: 603 357
BtsI GCAGTG_nn'
Cuts at: 0 400 540 960
Size: 400 140 420
Fragments arranged by size:
420 400 140
EaeI y'GGCC_r
Cuts at: 0 155 960
Size: 155 805
EarI CTCTTCn'nnn_
Cuts at: 0 118 328 960
Size: 118 210 632
Fragments arranged by size:
632 210 118
EcoO109I rG'GnC_Cy
Cuts at: 0 500 960
Size: 500 460
MslI CAynn'nnrTG
Cuts at: 0 130 358 622 628 960
Size: 130 228 264 6 332
Fragments arranged by size:
332 264 228 130 6
NcoI C'CATG_G
Cuts at: 0 623 960
Size: 623 337
NspI r_CATG'y
Cuts at: 0 341 960
Size: 341 619
PciI A'CATG_T
Cuts at: 0 337 960
Size: 337 623
PflMI CCAn_nnn'nTGG
Cuts at: 0 458 678 750 831 960
Size: 458 220 72 81 129
Fragments arranged by size:
458 220 129 81 72
PpuMI rG'GwC_Cy
Cuts at: 0 500 960
Size: 500 460
PstI C_TGCA'G
Cuts at: 0 314 739 960
Size: 314 425 221
Fragments arranged by size:
425 314 221
SfcI C'TryA_G
Cuts at: 0 310 735 798 960
Size: 310 425 63 162
Fragments arranged by size:
425 310 162 63
SmlI C'TyrA_G
Cuts at: 0 932 960
Size: 932 28
StuI AGG'CCT
Cuts at: 0 574 960
Size: 574 386
StyI C'CwwG_G
Cuts at: 0 218 443 623 960
Size: 218 225 180 337
Fragments arranged by size:
337 225 218 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 413 960
Size: 413 547
TatI w'GTAC_w
Cuts at: 0 191 960
Size: 191 769
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 789 960
Size: 441 348 171
Fragments arranged by size:
441 348 171
Enzymes that do cut:
AarI AflIII AlwNI BanI BanII BbsI BbvCI BclI
BglI Bme1580I Bpu10I BpuEI BsaI BsaAI BseRI BseYI
BsgI Bsp1286I BspHI BspMI BsrDI BstAPI BtgI BtgZI
BtsI EaeI EarI EcoO109I MslI NcoI NspI PciI
PflMI PpuMI PstI SfcI SmlI StuI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI
BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpmI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI