Restriction map for
pFN21AE6056
(Linear) (Six-base) MAPSORT of: KIEE6056.seq Check: 390 from: 1 to: 957
KIEE6056.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AgeI A'CCGG_T
Cuts at: 0 362 957
Size: 362 595
AleI CACnn'nnGTG
Cuts at: 0 419 809 957
Size: 419 390 148
Fragments arranged by size:
419 390 148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 957
Size: 416 32 509
Fragments arranged by size:
509 416 32
ApoI r'AATT_y
Cuts at: 0 23 31 957
Size: 23 8 926
Fragments arranged by size:
926 23 8
AvaI C'yCGr_G
Cuts at: 0 118 957
Size: 118 839
AvrII C'CTAG_G
Cuts at: 0 765 957
Size: 765 192
BamHI G'GATC_C
Cuts at: 0 638 957
Size: 638 319
BanII G_rGCy'C
Cuts at: 0 900 957
Size: 900 57
Bme1580I G_kGCm'C
Cuts at: 0 955 957
Size: 955 2
BmrI ACTGGGnnnn_n'
Cuts at: 0 644 957
Size: 644 313
Bpu10I CC'TnA_GC
Cuts at: 0 692 957
Size: 692 265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 957
Size: 792 165
BsaBI GATnn'nnATC
Cuts at: 0 250 957
Size: 250 707
BsaWI w'CCGG_w
Cuts at: 0 362 957
Size: 362 595
BseYI C'CCAG_C
Cuts at: 0 615 957
Size: 615 342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 489 957
Size: 373 116 468
Fragments arranged by size:
468 373 116
BsiHKAI G_wGCw'C
Cuts at: 0 119 459 957
Size: 119 340 498
Fragments arranged by size:
498 340 119
Bsp1286I G_dGCh'C
Cuts at: 0 119 459 900 955 957
Size: 119 340 441 55 2
Fragments arranged by size:
441 340 119 55 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 957
Size: 134 823
BsrFI r'CCGG_y
Cuts at: 0 362 957
Size: 362 595
BsrGI T'GTAC_A
Cuts at: 0 830 957
Size: 830 127
BstEII G'GTnAC_C
Cuts at: 0 840 957
Size: 840 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 957
Size: 105 852
BstYI r'GATC_y
Cuts at: 0 638 957
Size: 638 319
DraIII CAC_nnn'GTG
Cuts at: 0 591 957
Size: 591 366
EaeI y'GGCC_r
Cuts at: 0 140 371 957
Size: 140 231 586
Fragments arranged by size:
586 231 140
EcoO109I rG'GnC_Cy
Cuts at: 0 625 957
Size: 625 332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 579 957
Size: 215 364 378
Fragments arranged by size:
378 364 215
MscI TGG'CCA
Cuts at: 0 142 373 957
Size: 142 231 584
Fragments arranged by size:
584 231 142
MslI CAynn'nnrTG
Cuts at: 0 172 298 419 809 847 957
Size: 172 126 121 390 38 110
Fragments arranged by size:
390 172 126 121 110 38
NspI r_CATG'y
Cuts at: 0 679 957
Size: 679 278
PfoI T'CCnGG_A
Cuts at: 0 442 957
Size: 442 515
PpuMI rG'GwC_Cy
Cuts at: 0 625 957
Size: 625 332
SexAI A'CCwGG_T
Cuts at: 0 104 957
Size: 104 853
SfcI C'TryA_G
Cuts at: 0 867 957
Size: 867 90
SmlI C'TyrA_G
Cuts at: 0 807 957
Size: 807 150
StyI C'CwwG_G
Cuts at: 0 765 957
Size: 765 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 607 957
Size: 607 350
TatI w'GTAC_w
Cuts at: 0 176 701 830 957
Size: 176 525 129 127
Fragments arranged by size:
525 176 129 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 438 470 957
Size: 216 32 190 32 487
Fragments arranged by size:
487 216 190 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 35 957
Size: 35 922
Enzymes that do cut:
AgeI AleI AloI ApoI AvaI AvrII BamHI BanII
Bme1580I BmrI Bpu10I BpuEI BsaBI BsaWI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BsrGI BstEII BstXI BstYI
DraIII EaeI EcoO109I MmeI MscI MslI NspI PfoI
PpuMI SexAI SfcI SmlI StyI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI BsaI BsaAI BsaHI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI