Restriction map for
pFN21AE6054
(Linear) (Six-base) MAPSORT of: KIEE6054.seq Check: 7869 from: 1 to: 957
KIEE6054.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AflIII A'CryG_T
Cuts at: 0 671 957
Size: 671 286
AleI CACnn'nnGTG
Cuts at: 0 31 478 957
Size: 31 447 479
Fragments arranged by size:
479 447 31
ApaLI G'TGCA_C
Cuts at: 0 277 957
Size: 277 680
ApoI r'AATT_y
Cuts at: 0 49 947 957
Size: 49 898 10
Fragments arranged by size:
898 49 10
AseI AT'TA_AT
Cuts at: 0 607 957
Size: 607 350
BanII G_rGCy'C
Cuts at: 0 353 957
Size: 353 604
BciVI GTATCCnnnnn_n'
Cuts at: 0 571 957
Size: 571 386
BclI T'GATC_A
Cuts at: 0 416 761 957
Size: 416 345 196
Fragments arranged by size:
416 345 196
Bme1580I G_kGCm'C
Cuts at: 0 281 957
Size: 281 676
BmtI G_CTAG'C
Cuts at: 0 322 957
Size: 322 635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 957
Size: 690 267
BsaAI yAC'GTr
Cuts at: 0 387 414 957
Size: 387 27 543
Fragments arranged by size:
543 387 27
BsaBI GATnn'nnATC
Cuts at: 0 337 957
Size: 337 620
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 157 187 957
Size: 157 30 770
Fragments arranged by size:
770 157 30
BsiHKAI G_wGCw'C
Cuts at: 0 281 353 957
Size: 281 72 604
Fragments arranged by size:
604 281 72
BsmI GAATG_Cn'
Cuts at: 0 322 957
Size: 322 635
Bsp1286I G_dGCh'C
Cuts at: 0 281 353 957
Size: 281 72 604
Fragments arranged by size:
604 281 72
BsrDI GCAATG_nn'
Cuts at: 0 245 374 957
Size: 245 129 583
Fragments arranged by size:
583 245 129
Bsu36I CC'TnA_GG
Cuts at: 0 892 957
Size: 892 65
BtsI GCAGTG_nn'
Cuts at: 0 38 957
Size: 38 919
ClaI AT'CG_AT
Cuts at: 0 939 957
Size: 939 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 228 263 957
Size: 228 35 694
Fragments arranged by size:
694 228 35
DraI TTT'AAA
Cuts at: 0 510 957
Size: 510 447
EaeI y'GGCC_r
Cuts at: 0 686 957
Size: 686 271
EarI CTCTTCn'nnn_
Cuts at: 0 112 410 646 957
Size: 112 298 236 311
Fragments arranged by size:
311 298 236 112
EcoICRI GAG'CTC
Cuts at: 0 351 957
Size: 351 606
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 126 127 158 957
Size: 95 31 1 31 799
Fragments arranged by size:
799 95 31 31 1
MfeI C'AATT_G
Cuts at: 0 300 653 957
Size: 300 353 304
Fragments arranged by size:
353 304 300
MscI TGG'CCA
Cuts at: 0 688 957
Size: 688 269
MslI CAynn'nnrTG
Cuts at: 0 31 190 478 957
Size: 31 159 288 479
Fragments arranged by size:
479 288 159 31
NheI G'CTAG_C
Cuts at: 0 318 957
Size: 318 639
NspI r_CATG'y
Cuts at: 0 675 957
Size: 675 282
PasI CC'CwG_GG
Cuts at: 0 79 957
Size: 79 878
PciI A'CATG_T
Cuts at: 0 671 957
Size: 671 286
PflMI CCAn_nnn'nTGG
Cuts at: 0 100 957
Size: 100 857
PshAI GACnn'nnGTC
Cuts at: 0 213 957
Size: 213 744
PstI C_TGCA'G
Cuts at: 0 89 957
Size: 89 868
SacI G_AGCT'C
Cuts at: 0 353 957
Size: 353 604
SapI GCTCTTCn'nnn_
Cuts at: 0 646 957
Size: 646 311
ScaI AGT'ACT
Cuts at: 0 437 957
Size: 437 520
SfcI C'TryA_G
Cuts at: 0 85 468 738 885 957
Size: 85 383 270 147 72
Fragments arranged by size:
383 270 147 85 72
SmlI C'TyrA_G
Cuts at: 0 705 957
Size: 705 252
SnaBI TAC'GTA
Cuts at: 0 387 957
Size: 387 570
StyI C'CwwG_G
Cuts at: 0 38 957
Size: 38 919
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 432 957
Size: 432 525
TatI w'GTAC_w
Cuts at: 0 194 435 957
Size: 194 241 522
Fragments arranged by size:
522 241 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 816 957
Size: 816 141
XmnI GAAnn'nnTTC
Cuts at: 0 53 957
Size: 53 904
Enzymes that do cut:
AflIII AleI ApaLI ApoI AseI BanII BciVI BclI
Bme1580I BmtI BpuEI BsaAI BsaBI BsaXI BsiHKAI BsmI
Bsp1286I BsrDI Bsu36I BtsI ClaI CspCI DraI EaeI
EarI EcoICRI Hin4I MfeI MscI MslI NheI NspI
PasI PciI PflMI PshAI PstI SacI SapI ScaI
SfcI SmlI SnaBI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BfrBI BglI BglII BlpI BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI DraIII DrdI EagI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI