Restriction map for
pFN21AE6053
(Linear) (Six-base) MAPSORT of: KIEE6053.seq Check: 7010 from: 1 to: 954
KIEE6053.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 954
Size: 724 230
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 954
Size: 694 260
AflIII A'CryG_T
Cuts at: 0 206 954
Size: 206 748
AleI CACnn'nnGTG
Cuts at: 0 731 954
Size: 731 223
ApaI G_GGCC'C
Cuts at: 0 509 954
Size: 509 445
ApaLI G'TGCA_C
Cuts at: 0 417 954
Size: 417 537
ApoI r'AATT_y
Cuts at: 0 476 536 954
Size: 476 60 418
Fragments arranged by size:
476 418 60
AvaI C'yCGr_G
Cuts at: 0 509 661 954
Size: 509 152 293
Fragments arranged by size:
509 293 152
BanI G'GyrC_C
Cuts at: 0 892 954
Size: 892 62
BanII G_rGCy'C
Cuts at: 0 509 954
Size: 509 445
BbeI G_GCGC'C
Cuts at: 0 896 954
Size: 896 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 87 954
Size: 87 867
BfrBI ATG'CAT
Cuts at: 0 287 954
Size: 287 667
Bme1580I G_kGCm'C
Cuts at: 0 421 509 954
Size: 421 88 445
Fragments arranged by size:
445 421 88
BmgBI CAC'GTC
Cuts at: 0 437 954
Size: 437 517
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 896 954
Size: 896 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 895 954
Size: 429 466 59
Fragments arranged by size:
466 429 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 954
Size: 535 419
BsaI GGTCTCn'nnnn_
Cuts at: 0 648 954
Size: 648 306
BsaHI Gr'CG_yC
Cuts at: 0 893 954
Size: 893 61
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 711 814 954
Size: 64 647 103 140
Fragments arranged by size:
647 140 103 64
BseYI C'CCAG_C
Cuts at: 0 39 114 954
Size: 39 75 840
Fragments arranged by size:
840 75 39
BsiHKAI G_wGCw'C
Cuts at: 0 421 909 954
Size: 421 488 45
Fragments arranged by size:
488 421 45
BsmI GAATG_Cn'
Cuts at: 0 882 954
Size: 882 72
Bsp1286I G_dGCh'C
Cuts at: 0 421 509 909 954
Size: 421 88 400 45
Fragments arranged by size:
421 400 88 45
BspHI T'CATG_A
Cuts at: 0 401 954
Size: 401 553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 954
Size: 724 230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 954
Size: 240 714
BstYI r'GATC_y
Cuts at: 0 677 954
Size: 677 277
Bsu36I CC'TnA_GG
Cuts at: 0 871 954
Size: 871 83
BtgI C'CryG_G
Cuts at: 0 761 954
Size: 761 193
EaeI y'GGCC_r
Cuts at: 0 194 368 954
Size: 194 174 586
Fragments arranged by size:
586 194 174
EarI CTCTTCn'nnn_
Cuts at: 0 91 532 913 954
Size: 91 441 381 41
Fragments arranged by size:
441 381 91 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 694 895 954
Size: 429 265 201 59
Fragments arranged by size:
429 265 201 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 357 954
Size: 357 597
EcoO109I rG'GnC_Cy
Cuts at: 0 506 954
Size: 506 448
HaeII r_GCGC'y
Cuts at: 0 896 954
Size: 896 58
HincII GTy'rAC
Cuts at: 0 579 954
Size: 579 375
HindIII A'AGCT_T
Cuts at: 0 157 954
Size: 157 797
HpaI GTT'AAC
Cuts at: 0 579 954
Size: 579 375
KasI G'GCGC_C
Cuts at: 0 892 954
Size: 892 62
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 720 954
Size: 245 475 234
Fragments arranged by size:
475 245 234
MscI TGG'CCA
Cuts at: 0 196 370 954
Size: 196 174 584
Fragments arranged by size:
584 196 174
MslI CAynn'nnrTG
Cuts at: 0 169 205 731 760 954
Size: 169 36 526 29 194
Fragments arranged by size:
526 194 169 36 29
NarI GG'CG_CC
Cuts at: 0 893 954
Size: 893 61
NcoI C'CATG_G
Cuts at: 0 761 954
Size: 761 193
NsiI A_TGCA'T
Cuts at: 0 289 954
Size: 289 665
NspI r_CATG'y
Cuts at: 0 210 654 954
Size: 210 444 300
Fragments arranged by size:
444 300 210
PciI A'CATG_T
Cuts at: 0 206 954
Size: 206 748
PspOMI G'GGCC_C
Cuts at: 0 505 954
Size: 505 449
SfcI C'TryA_G
Cuts at: 0 864 954
Size: 864 90
SfoI GGC'GCC
Cuts at: 0 894 954
Size: 894 60
SmaI CCC'GGG
Cuts at: 0 511 954
Size: 511 443
SmlI C'TyrA_G
Cuts at: 0 550 954
Size: 550 404
StuI AGG'CCT
Cuts at: 0 706 954
Size: 706 248
StyI C'CwwG_G
Cuts at: 0 761 954
Size: 761 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 954
Size: 183 771
TatI w'GTAC_w
Cuts at: 0 173 389 954
Size: 173 216 565
Fragments arranged by size:
565 216 173
XmaI C'CCGG_G
Cuts at: 0 509 954
Size: 509 445
Enzymes that do cut:
AarI AcuI AflIII AleI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BfrBI Bme1580I BmgBI BplI
BpmI BpuEI BsaI BsaHI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspHI BspMI BstXI BstYI Bsu36I BtgI EaeI
EarI Eco57MI EcoNI EcoO109I HaeII HincII HindIII HpaI
KasI MmeI MscI MslI NarI NcoI NsiI NspI
PciI PspOMI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BglI BglII BlpI
BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI BsgI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I KpnI
MfeI MluI MspA1I NaeI NdeI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI