Restriction map for
pFN21AE6049
(Linear) (Six-base) MAPSORT of: KIEE6049.seq Check: 8529 from: 1 to: 954
KIEE6049.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 742 954
Size: 742 212
AatII G_ACGT'C
Cuts at: 0 590 954
Size: 590 364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 954
Size: 589 365
AleI CACnn'nnGTG
Cuts at: 0 862 954
Size: 862 92
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 646 954
Size: 646 308
AlwNI CAG_nnn'CTG
Cuts at: 0 444 954
Size: 444 510
ApoI r'AATT_y
Cuts at: 0 699 954
Size: 699 255
AvaI C'yCGr_G
Cuts at: 0 798 954
Size: 798 156
BamHI G'GATC_C
Cuts at: 0 308 954
Size: 308 646
BanII G_rGCy'C
Cuts at: 0 173 915 954
Size: 173 742 39
Fragments arranged by size:
742 173 39
BbvCI CC'TCA_GC
Cuts at: 0 344 954
Size: 344 610
BclI T'GATC_A
Cuts at: 0 749 954
Size: 749 205
BglI GCCn_nnn'nGGC
Cuts at: 0 460 954
Size: 460 494
BlpI GC'TnA_GC
Cuts at: 0 818 954
Size: 818 136
Bme1580I G_kGCm'C
Cuts at: 0 251 954
Size: 251 703
BmgBI CAC'GTC
Cuts at: 0 828 954
Size: 828 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 143 954
Size: 143 811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 359 642 954
Size: 296 63 283 312
Fragments arranged by size:
312 296 283 63
Bpu10I CC'TnA_GC
Cuts at: 0 6 344 954
Size: 6 338 610
Fragments arranged by size:
610 338 6
BsaAI yAC'GTr
Cuts at: 0 611 954
Size: 611 343
BsaHI Gr'CG_yC
Cuts at: 0 587 954
Size: 587 367
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 332 362 954
Size: 332 30 592
Fragments arranged by size:
592 332 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 136 145 933 954
Size: 82 54 9 788 21
Fragments arranged by size:
788 82 54 21 9
BseYI C'CCAG_C
Cuts at: 0 458 954
Size: 458 496
BsiHKAI G_wGCw'C
Cuts at: 0 7 954
Size: 7 947
BsmI GAATG_Cn'
Cuts at: 0 144 954
Size: 144 810
BsmBI CGTCTCn'nnnn_
Cuts at: 0 594 954
Size: 594 360
Bsp1286I G_dGCh'C
Cuts at: 0 7 173 251 915 954
Size: 7 166 78 664 39
Fragments arranged by size:
664 166 78 39 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 742 954
Size: 742 212
BstXI CCAn_nnnn'nTGG
Cuts at: 0 274 954
Size: 274 680
BstYI r'GATC_y
Cuts at: 0 222 308 954
Size: 222 86 646
Fragments arranged by size:
646 222 86
BtgI C'CryG_G
Cuts at: 0 365 954
Size: 365 589
BtsI GCAGTG_nn'
Cuts at: 0 412 954
Size: 412 542
EaeI y'GGCC_r
Cuts at: 0 711 728 954
Size: 711 17 226
Fragments arranged by size:
711 226 17
EarI CTCTTCn'nnn_
Cuts at: 0 769 954
Size: 769 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 359 589 642 954
Size: 296 63 230 53 312
Fragments arranged by size:
312 296 230 63 53
EcoRI G'AATT_C
Cuts at: 0 699 954
Size: 699 255
MscI TGG'CCA
Cuts at: 0 730 954
Size: 730 224
MslI CAynn'nnrTG
Cuts at: 0 187 502 628 790 862 954
Size: 187 315 126 162 72 92
Fragments arranged by size:
315 187 162 126 92 72
MspA1I CmG'CkG
Cuts at: 0 709 817 954
Size: 709 108 137
Fragments arranged by size:
709 137 108
NcoI C'CATG_G
Cuts at: 0 365 954
Size: 365 589
NspI r_CATG'y
Cuts at: 0 85 795 954
Size: 85 710 159
Fragments arranged by size:
710 159 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 444 954
Size: 444 510
PvuII CAG'CTG
Cuts at: 0 817 954
Size: 817 137
SapI GCTCTTCn'nnn_
Cuts at: 0 769 954
Size: 769 185
SphI G_CATG'C
Cuts at: 0 85 954
Size: 85 869
StuI AGG'CCT
Cuts at: 0 724 954
Size: 724 230
StyI C'CwwG_G
Cuts at: 0 365 725 954
Size: 365 360 229
Fragments arranged by size:
365 360 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 392 954
Size: 392 562
TatI w'GTAC_w
Cuts at: 0 191 954
Size: 191 763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 954
Size: 258 696
ZraI GAC'GTC
Cuts at: 0 588 954
Size: 588 366
Enzymes that do cut:
AarI AatII AcuI AleI AlfI AlwNI ApoI AvaI
BamHI BanII BbvCI BclI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BstXI BstYI BtgI
BtsI EaeI EarI Eco57MI EcoRI MscI MslI MspA1I
NcoI NspI PflMI PvuII SapI SphI StuI StyI
TaqII TatI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbsI BcgI BciVI BfrBI BglII BmtI
BplI BpuEI BsaI BsaBI BsaWI BsgI BsiEI BsiWI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI