Restriction map for
pFN21AE6047
(Linear) (Six-base) MAPSORT of: KIEE6047.seq Check: 5844 from: 1 to: 954
KIEE6047.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
Acc65I G'GTAC_C
Cuts at: 0 413 954
Size: 413 541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 954
Size: 756 198
AflIII A'CryG_T
Cuts at: 0 739 954
Size: 739 215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 799 954
Size: 799 155
AlwNI CAG_nnn'CTG
Cuts at: 0 49 195 954
Size: 49 146 759
Fragments arranged by size:
759 146 49
ApoI r'AATT_y
Cuts at: 0 52 926 954
Size: 52 874 28
Fragments arranged by size:
874 52 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 397 430 954
Size: 397 33 524
Fragments arranged by size:
524 397 33
BanI G'GyrC_C
Cuts at: 0 413 954
Size: 413 541
BanII G_rGCy'C
Cuts at: 0 646 954
Size: 646 308
BbsI GAAGACnn'nnnn_
Cuts at: 0 450 954
Size: 450 504
BbvCI CC'TCA_GC
Cuts at: 0 112 954
Size: 112 842
BclI T'GATC_A
Cuts at: 0 158 954
Size: 158 796
BlpI GC'TnA_GC
Cuts at: 0 208 954
Size: 208 746
BmrI ACTGGGnnnn_n'
Cuts at: 0 37 954
Size: 37 917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 301 954
Size: 193 108 653
Fragments arranged by size:
653 193 108
Bpu10I CC'TnA_GC
Cuts at: 0 112 733 954
Size: 112 621 221
Fragments arranged by size:
621 221 112
BsaBI GATnn'nnATC
Cuts at: 0 163 325 954
Size: 163 162 629
Fragments arranged by size:
629 163 162
BsaHI Gr'CG_yC
Cuts at: 0 16 954
Size: 16 938
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 954
Size: 85 30 839
Fragments arranged by size:
839 85 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 205 954
Size: 49 156 749
Fragments arranged by size:
749 156 49
BseYI C'CCAG_C
Cuts at: 0 22 639 954
Size: 22 617 315
Fragments arranged by size:
617 315 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 342 473 954
Size: 169 173 131 481
Fragments arranged by size:
481 173 169 131
BsiHKAI G_wGCw'C
Cuts at: 0 239 739 954
Size: 239 500 215
Fragments arranged by size:
500 239 215
BsmI GAATG_Cn'
Cuts at: 0 480 954
Size: 480 474
Bsp1286I G_dGCh'C
Cuts at: 0 239 646 739 954
Size: 239 407 93 215
Fragments arranged by size:
407 239 215 93
BspHI T'CATG_A
Cuts at: 0 329 954
Size: 329 625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 954
Size: 212 742
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 264 526 786 954
Size: 171 93 262 260 168
Fragments arranged by size:
262 260 171 168 93
BtgI C'CryG_G
Cuts at: 0 134 347 827 954
Size: 134 213 480 127
Fragments arranged by size:
480 213 134 127
BtsI GCAGTG_nn'
Cuts at: 0 406 728 954
Size: 406 322 226
Fragments arranged by size:
406 322 226
EaeI y'GGCC_r
Cuts at: 0 131 266 392 443 809 954
Size: 131 135 126 51 366 145
Fragments arranged by size:
366 145 135 131 126 51
EarI CTCTTCn'nnn_
Cuts at: 0 127 954
Size: 127 827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 301 756 954
Size: 193 108 455 198
Fragments arranged by size:
455 198 193 108
EcoO109I rG'GnC_Cy
Cuts at: 0 261 729 822 954
Size: 261 468 93 132
Fragments arranged by size:
468 261 132 93
EcoRI G'AATT_C
Cuts at: 0 52 926 954
Size: 52 874 28
Fragments arranged by size:
874 52 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 954
Size: 274 32 648
Fragments arranged by size:
648 274 32
HincII GTy'rAC
Cuts at: 0 800 954
Size: 800 154
KpnI G_GTAC'C
Cuts at: 0 417 954
Size: 417 537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 954
Size: 610 344
MscI TGG'CCA
Cuts at: 0 133 268 394 445 811 954
Size: 133 135 126 51 366 143
Fragments arranged by size:
366 143 135 133 126 51
MslI CAynn'nnrTG
Cuts at: 0 364 466 524 954
Size: 364 102 58 430
Fragments arranged by size:
430 364 102 58
MspA1I CmG'CkG
Cuts at: 0 171 954
Size: 171 783
NcoI C'CATG_G
Cuts at: 0 134 347 827 954
Size: 134 213 480 127
Fragments arranged by size:
480 213 134 127
NspI r_CATG'y
Cuts at: 0 743 954
Size: 743 211
PciI A'CATG_T
Cuts at: 0 739 954
Size: 739 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 275 621 954
Size: 275 346 333
Fragments arranged by size:
346 333 275
PpuMI rG'GwC_Cy
Cuts at: 0 261 822 954
Size: 261 561 132
Fragments arranged by size:
561 261 132
PstI C_TGCA'G
Cuts at: 0 944 954
Size: 944 10
PvuII CAG'CTG
Cuts at: 0 171 954
Size: 171 783
SfcI C'TryA_G
Cuts at: 0 940 954
Size: 940 14
StuI AGG'CCT
Cuts at: 0 178 954
Size: 178 776
StyI C'CwwG_G
Cuts at: 0 134 257 347 827 954
Size: 134 123 90 480 127
Fragments arranged by size:
480 134 127 123 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 36 419 507 954
Size: 36 383 88 447
Fragments arranged by size:
447 383 88 36
TatI w'GTAC_w
Cuts at: 0 197 954
Size: 197 757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 954
Size: 85 32 837
Fragments arranged by size:
837 85 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 622 954
Size: 622 332
XmnI GAAnn'nnTTC
Cuts at: 0 337 954
Size: 337 617
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AlwNI ApoI BaeI BanI
BanII BbsI BbvCI BclI BlpI BmrI BpmI Bpu10I
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BstXI BtgI BtsI EaeI EarI
Eco57MI EcoO109I EcoRI Hin4I HincII KpnI MmeI MscI
MslI MspA1I NcoI NspI PciI PflMI PpuMI PstI
PvuII SfcI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BcgI BciVI BfrBI BglI BglII
Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaAI BsaWI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI ZraI