Restriction map for
pFN21AE6044
(Linear) (Six-base) MAPSORT of: KIEE6044.seq Check: 657 from: 1 to: 951
KIEE6044.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AflIII A'CryG_T
Cuts at: 0 716 718 951
Size: 716 2 233
Fragments arranged by size:
716 233 2
AleI CACnn'nnGTG
Cuts at: 0 719 951
Size: 719 232
AlwNI CAG_nnn'CTG
Cuts at: 0 59 243 951
Size: 59 184 708
Fragments arranged by size:
708 184 59
ApaLI G'TGCA_C
Cuts at: 0 63 504 951
Size: 63 441 447
Fragments arranged by size:
447 441 63
ApoI r'AATT_y
Cuts at: 0 31 919 951
Size: 31 888 32
Fragments arranged by size:
888 32 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 819 951
Size: 819 132
BclI T'GATC_A
Cuts at: 0 143 522 951
Size: 143 379 429
Fragments arranged by size:
429 379 143
BfrBI ATG'CAT
Cuts at: 0 408 951
Size: 408 543
Bme1580I G_kGCm'C
Cuts at: 0 67 508 951
Size: 67 441 443
Fragments arranged by size:
443 441 67
BmgBI CAC'GTC
Cuts at: 0 20 951
Size: 20 931
BmrI ACTGGGnnnn_n'
Cuts at: 0 291 951
Size: 291 660
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 939 951
Size: 939 12
BsaAI yAC'GTr
Cuts at: 0 719 951
Size: 719 232
BsaWI w'CCGG_w
Cuts at: 0 656 951
Size: 656 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 951
Size: 562 30 359
Fragments arranged by size:
562 359 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 567 951
Size: 567 384
BseYI C'CCAG_C
Cuts at: 0 117 236 942 951
Size: 117 119 706 9
Fragments arranged by size:
706 119 117 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 493 951
Size: 311 182 458
Fragments arranged by size:
458 311 182
BsiHKAI G_wGCw'C
Cuts at: 0 67 508 951
Size: 67 441 443
Fragments arranged by size:
443 441 67
Bsp1286I G_dGCh'C
Cuts at: 0 67 508 951
Size: 67 441 443
Fragments arranged by size:
443 441 67
BspEI T'CCGG_A
Cuts at: 0 656 951
Size: 656 295
BspHI T'CATG_A
Cuts at: 0 107 951
Size: 107 844
BstYI r'GATC_y
Cuts at: 0 680 951
Size: 680 271
BtsI GCAGTG_nn'
Cuts at: 0 353 951
Size: 353 598
EaeI y'GGCC_r
Cuts at: 0 398 431 951
Size: 398 33 520
Fragments arranged by size:
520 398 33
EcoO109I rG'GnC_Cy
Cuts at: 0 896 951
Size: 896 55
EcoRV GAT'ATC
Cuts at: 0 74 951
Size: 74 877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 951
Size: 726 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 562 594 855 887 951
Size: 514 32 16 32 261 32 64
Fragments arranged by size:
514 261 64 32 32 32 16
HincII GTy'rAC
Cuts at: 0 932 951
Size: 932 19
HpaI GTT'AAC
Cuts at: 0 932 951
Size: 932 19
MscI TGG'CCA
Cuts at: 0 433 951
Size: 433 518
MslI CAynn'nnrTG
Cuts at: 0 112 172 598 719 841 951
Size: 112 60 426 121 122 110
Fragments arranged by size:
426 122 121 112 110 60
MspA1I CmG'CkG
Cuts at: 0 240 495 951
Size: 240 255 456
Fragments arranged by size:
456 255 240
NsiI A_TGCA'T
Cuts at: 0 410 951
Size: 410 541
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 243 951
Size: 59 184 708
Fragments arranged by size:
708 184 59
PmlI CAC'GTG
Cuts at: 0 719 951
Size: 719 232
PvuII CAG'CTG
Cuts at: 0 240 495 951
Size: 240 255 456
Fragments arranged by size:
456 255 240
StyI C'CwwG_G
Cuts at: 0 42 317 951
Size: 42 275 634
Fragments arranged by size:
634 275 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 12 310 951
Size: 12 298 641
Fragments arranged by size:
641 298 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 787 951
Size: 787 164
XmnI GAAnn'nnTTC
Cuts at: 0 35 709 951
Size: 35 674 242
Fragments arranged by size:
674 242 35
Enzymes that do cut:
AflIII AleI AlwNI ApaLI ApoI BbsI BclI BfrBI
Bme1580I BmgBI BmrI BplI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BstYI BtsI
EaeI EcoO109I EcoRV FalI Hin4I HincII HpaI MscI
MslI MspA1I NsiI PflMI PmlI PvuII StyI TaqII
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BglI BglII BlpI BmtI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI ZraI