(Linear) (Six-base) MAPSORT of: KIEE6041.seq  Check: 5185  from: 1  to: 951



KIEE6041.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:20  ..

AccI GT'mk_AC

Cuts at:      0     217     951 
   Size:        217     734
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     697     951 
   Size:        280     417     254

  Fragments arranged by size: 

                 417     280     254
 
AlwNI CAG_nnn'CTG

Cuts at:      0      74     951 
   Size:         74     877
 
ApoI r'AATT_y

Cuts at:      0      31     951 
   Size:         31     920
 
AseI AT'TA_AT

Cuts at:      0     605     951 
   Size:        605     346
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     160     193     951 
   Size:        160      33     758

  Fragments arranged by size: 

                 758     160      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     865     951 
   Size:        865      86
 
BglI GCCn_nnn'nGGC

Cuts at:      0     430     951 
   Size:        430     521
 
BlpI GC'TnA_GC

Cuts at:      0     553     951 
   Size:        553     398
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     199     229     951 
   Size:        199      30     722

  Fragments arranged by size: 

                 722     199      30
 
BseYI C'CCAG_C

Cuts at:      0     491     951 
   Size:        491     460
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     951 
   Size:        148     803
 
BsmI GAATG_Cn'

Cuts at:      0     337     951 
   Size:        337     614
 
BspHI T'CATG_A

Cuts at:      0     641     951 
   Size:        641     310
 
BstZ17I GTA'TAC

Cuts at:      0     218     951 
   Size:        218     733
 
BtgI C'CryG_G

Cuts at:      0     350     951 
   Size:        350     601
 
DraIII CAC_nnn'GTG

Cuts at:      0     421     951 
   Size:        421     530
 
EaeI y'GGCC_r

Cuts at:      0     371     951 
   Size:        371     580
 
EarI CTCTTCn'nnn_

Cuts at:      0     784     951 
   Size:        784     167
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     697     951 
   Size:        280     417     254

  Fragments arranged by size: 

                 417     280     254
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     726     951 
   Size:        726     225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     424     546     951 
   Size:        171     253     122     405

  Fragments arranged by size: 

                 405     253     171     122
 
MscI TGG'CCA

Cuts at:      0     373     951 
   Size:        373     578
 
MslI CAynn'nnrTG

Cuts at:      0     172     589     769     775     951 
   Size:        172     417     180       6     176

  Fragments arranged by size: 

                 417     180     176     172       6
 
NcoI C'CATG_G

Cuts at:      0     350     951 
   Size:        350     601
 
NspI r_CATG'y

Cuts at:      0     129     951 
   Size:        129     822
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318     951 
   Size:        318     633
 
SapI GCTCTTCn'nnn_

Cuts at:      0     784     951 
   Size:        784     167
 
SfcI C'TryA_G

Cuts at:      0     824     867     951 
   Size:        824      43      84

  Fragments arranged by size: 

                 824      84      43
 
SspI AAT'ATT

Cuts at:      0     252     951 
   Size:        252     699
 
StuI AGG'CCT

Cuts at:      0     709     951 
   Size:        709     242
 
StyI C'CwwG_G

Cuts at:      0      42     317     350     951 
   Size:         42     275      33     601

  Fragments arranged by size: 

                 601     275      42      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     827     951 
   Size:        827     124
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AseI     BaeI     BbsI     BglI
     BlpI    BsaXI    BseYI     BsgI     BsmI    BspHI  BstZ17I     BtgI
   DraIII     EaeI     EarI  Eco57MI     FalI     MmeI     MscI     MslI
     NcoI     NspI    PflMI     SapI     SfcI     SspI     StuI     StyI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI