(Linear) (Six-base) MAPSORT of: KIEE6036.seq  Check: 6250  from: 1  to: 951



KIEE6036.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:20  ..

AclI AA'CG_TT

Cuts at:      0     269     951 
   Size:        269     682
 
ApoI r'AATT_y

Cuts at:      0     918     951 
   Size:        918      33
 
BamHI G'GATC_C

Cuts at:      0     633     951 
   Size:        633     318
 
BanI G'GyrC_C

Cuts at:      0     852     951 
   Size:        852      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     332     951 
   Size:        332     619
 
BfrBI ATG'CAT

Cuts at:      0     942     951 
   Size:        942       9
 
BglI GCCn_nnn'nGGC

Cuts at:      0     697     951 
   Size:        697     254
 
BlpI GC'TnA_GC

Cuts at:      0     701     951 
   Size:        701     250
 
Bme1580I G_kGCm'C

Cuts at:      0     688     717     951 
   Size:        688      29     234

  Fragments arranged by size: 

                 688     234      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     446     951 
   Size:        446     505
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     951 
   Size:        478     473
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     226     951 
   Size:        226     725
 
BsaBI GATnn'nnATC

Cuts at:      0      88     951 
   Size:         88     863
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     100     130     951 
   Size:        100      30     821

  Fragments arranged by size: 

                 821     100      30
 
BsmI GAATG_Cn'

Cuts at:      0     942     951 
   Size:        942       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     688     717     951 
   Size:        688      29     234

  Fragments arranged by size: 

                 688     234      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     854     951 
   Size:        854      97
 
BstYI r'GATC_y

Cuts at:      0      83     633     899     951 
   Size:         83     550     266      52

  Fragments arranged by size: 

                 550     266      83      52
 
BtgI C'CryG_G

Cuts at:      0     767     951 
   Size:        767     184
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     775     951 
   Size:        775     176
 
EaeI y'GGCC_r

Cuts at:      0     377     833     951 
   Size:        377     456     118

  Fragments arranged by size: 

                 456     377     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     550     951 
   Size:        550     401
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     547     951 
   Size:        547     404
 
HincII GTy'rAC

Cuts at:      0     366     951 
   Size:        366     585
 
MscI TGG'CCA

Cuts at:      0     379     835     951 
   Size:        379     456     116

  Fragments arranged by size: 

                 456     379     116
 
MslI CAynn'nnrTG

Cuts at:      0     247     766     951 
   Size:        247     519     185

  Fragments arranged by size: 

                 519     247     185
 
MspA1I CmG'CkG

Cuts at:      0     593     951 
   Size:        593     358
 
NcoI C'CATG_G

Cuts at:      0     767     951 
   Size:        767     184
 
NsiI A_TGCA'T

Cuts at:      0     944     951 
   Size:        944       7
 
NspI r_CATG'y

Cuts at:      0     183     808     951 
   Size:        183     625     143

  Fragments arranged by size: 

                 625     183     143
 
PshAI GACnn'nnGTC

Cuts at:      0     218     951 
   Size:        218     733
 
PvuII CAG'CTG

Cuts at:      0     593     951 
   Size:        593     358
 
SmlI C'TyrA_G

Cuts at:      0     493     951 
   Size:        493     458
 
SphI G_CATG'C

Cuts at:      0     808     951 
   Size:        808     143
 
StuI AGG'CCT

Cuts at:      0      70     292     951 
   Size:         70     222     659

  Fragments arranged by size: 

                 659     222      70
 
StyI C'CwwG_G

Cuts at:      0     767     951 
   Size:        767     184
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     951 
   Size:        120     831
 

 Enzymes that do cut:

     AclI     ApoI    BamHI     BanI     BbsI    BfrBI     BglI     BlpI
 Bme1580I     BmrI    BpuEI     BsaI    BsaBI    BsaXI     BsmI Bsp1286I
    BspMI    BstYI     BtgI    BtgZI     EaeI     EciI    EcoNI   HincII
     MscI     MslI   MspA1I     NcoI     NsiI     NspI    PshAI    PvuII
     SmlI     SphI     StuI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BglII    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI