(Linear) (Six-base) MAPSORT of: KIEE6034.seq  Check: 8501  from: 1  to: 948



KIEE6034.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     948 
   Size:         88     860
 
AflII C'TTAA_G

Cuts at:      0     900     948 
   Size:        900      48
 
AleI CACnn'nnGTG

Cuts at:      0     115     948 
   Size:        115     833
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403     948 
   Size:        403     545
 
ApoI r'AATT_y

Cuts at:      0     786     908     948 
   Size:        786     122      40

  Fragments arranged by size: 

                 786     122      40
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     376     409     948 
   Size:        376      33     539

  Fragments arranged by size: 

                 539     376      33
 
BanI G'GyrC_C

Cuts at:      0     439     948 
   Size:        439     509
 
BbeI G_GCGC'C

Cuts at:      0     443     948 
   Size:        443     505
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      76     948 
   Size:         76     872
 
BclI T'GATC_A

Cuts at:      0     107     948 
   Size:        107     841
 
Bpu10I CC'TnA_GC

Cuts at:      0      62     948 
   Size:         62     886
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     914     948 
   Size:        914      34
 
BsaHI Gr'CG_yC

Cuts at:      0     440     948 
   Size:        440     508
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     948 
   Size:        311     637
 
BsmI GAATG_Cn'

Cuts at:      0     422     462     948 
   Size:        422      40     486

  Fragments arranged by size: 

                 486     422      40
 
BspHI T'CATG_A

Cuts at:      0     401     948 
   Size:        401     547
 
BsrGI T'GTAC_A

Cuts at:      0     176     948 
   Size:        176     772
 
BstYI r'GATC_y

Cuts at:      0     757     948 
   Size:        757     191
 
BtgI C'CryG_G

Cuts at:      0     113     948 
   Size:        113     835
 
BtsI GCAGTG_nn'

Cuts at:      0     356     385     948 
   Size:        356      29     563

  Fragments arranged by size: 

                 563     356      29
 
EaeI y'GGCC_r

Cuts at:      0     371     948 
   Size:        371     577
 
EarI CTCTTCn'nnn_

Cuts at:      0     548     948 
   Size:        548     400
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     948 
   Size:         88     860
 
HaeII r_GCGC'y

Cuts at:      0     443     948 
   Size:        443     505
 
HincII GTy'rAC

Cuts at:      0     570     948 
   Size:        570     378
 
KasI G'GCGC_C

Cuts at:      0     439     948 
   Size:        439     509
 
MscI TGG'CCA

Cuts at:      0     373     948 
   Size:        373     575
 
MslI CAynn'nnrTG

Cuts at:      0     115     172     403     427     948 
   Size:        115      57     231      24     521

  Fragments arranged by size: 

                 521     231     115      57      24
 
MspA1I CmG'CkG

Cuts at:      0     820     948 
   Size:        820     128
 
NarI GG'CG_CC

Cuts at:      0     440     948 
   Size:        440     508
 
PstI C_TGCA'G

Cuts at:      0     330     392     948 
   Size:        330      62     556

  Fragments arranged by size: 

                 556     330      62
 
PvuII CAG'CTG

Cuts at:      0     820     948 
   Size:        820     128
 
SfcI C'TryA_G

Cuts at:      0     326     388     948 
   Size:        326      62     560

  Fragments arranged by size: 

                 560     326      62
 
SfoI GGC'GCC

Cuts at:      0     441     948 
   Size:        441     507
 
SmlI C'TyrA_G

Cuts at:      0     900     929     948 
   Size:        900      29      19

  Fragments arranged by size: 

                 900      29      19
 
SspI AAT'ATT

Cuts at:      0     126     948 
   Size:        126     822
 
StyI C'CwwG_G

Cuts at:      0     689     948 
   Size:        689     259
 
TatI w'GTAC_w

Cuts at:      0     176     294     948 
   Size:        176     118     654

  Fragments arranged by size: 

                 654     176     118
 
XmnI GAAnn'nnTTC

Cuts at:      0     590     948 
   Size:        590     358
 

 Enzymes that do cut:

     AcuI    AflII     AleI     AlfI     ApoI     BaeI     BanI     BbeI
     BbsI     BclI   Bpu10I    BpuEI    BsaHI     BsgI     BsmI    BspHI
    BsrGI    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI    HaeII
   HincII     KasI     MscI     MslI   MspA1I     NarI     PstI    PvuII
     SfcI     SfoI     SmlI     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI    BanII    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI