Restriction map for
pFN21AE6033
(Linear) (Six-base) MAPSORT of: KIEE6033.seq Check: 7032 from: 1 to: 948
KIEE6033.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 243 419 948
Size: 243 176 529
Fragments arranged by size:
529 243 176
BanI G'GyrC_C
Cuts at: 0 540 948
Size: 540 408
BanII G_rGCy'C
Cuts at: 0 325 948
Size: 325 623
BbvCI CC'TCA_GC
Cuts at: 0 62 187 948
Size: 62 125 761
Fragments arranged by size:
761 125 62
BciVI GTATCCnnnnn_n'
Cuts at: 0 564 948
Size: 564 384
BglI GCCn_nnn'nGGC
Cuts at: 0 352 948
Size: 352 596
BlpI GC'TnA_GC
Cuts at: 0 448 948
Size: 448 500
BmrI ACTGGGnnnn_n'
Cuts at: 0 925 948
Size: 925 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 463 948
Size: 463 485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 948
Size: 141 807
Bpu10I CC'TnA_GC
Cuts at: 0 62 187 948
Size: 62 125 761
Fragments arranged by size:
761 125 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 650 921 948
Size: 129 521 271 27
Fragments arranged by size:
521 271 129 27
BsaAI yAC'GTr
Cuts at: 0 306 948
Size: 306 642
BseYI C'CCAG_C
Cuts at: 0 296 948
Size: 296 652
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 948
Size: 511 437
BsiHKAI G_wGCw'C
Cuts at: 0 481 551 948
Size: 481 70 397
Fragments arranged by size:
481 397 70
BsmBI CGTCTCn'nnnn_
Cuts at: 0 431 731 948
Size: 431 300 217
Fragments arranged by size:
431 300 217
Bsp1286I G_dGCh'C
Cuts at: 0 325 481 551 948
Size: 325 156 70 397
Fragments arranged by size:
397 325 156 70
BsrFI r'CCGG_y
Cuts at: 0 380 433 783 948
Size: 380 53 350 165
Fragments arranged by size:
380 350 165 53
BssSI C'ACGA_G
Cuts at: 0 198 948
Size: 198 750
BstEII G'GTnAC_C
Cuts at: 0 246 948
Size: 246 702
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 948
Size: 318 630
BstYI r'GATC_y
Cuts at: 0 796 948
Size: 796 152
BtgI C'CryG_G
Cuts at: 0 311 948
Size: 311 637
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 496 948
Size: 496 452
BtsI GCAGTG_nn'
Cuts at: 0 125 385 948
Size: 125 260 563
Fragments arranged by size:
563 260 125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 210 245 948
Size: 210 35 703
Fragments arranged by size:
703 210 35
DraI TTT'AAA
Cuts at: 0 854 948
Size: 854 94
DrdI GACnn_nn'nnGTC
Cuts at: 0 723 948
Size: 723 225
EaeI y'GGCC_r
Cuts at: 0 308 344 382 431 602 785 948
Size: 308 36 38 49 171 183 163
Fragments arranged by size:
308 183 171 163 49 38 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 948
Size: 141 807
EcoO109I rG'GnC_Cy
Cuts at: 0 716 896 905 948
Size: 716 180 9 43
Fragments arranged by size:
716 180 43 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 948
Size: 23 32 893
Fragments arranged by size:
893 32 23
MscI TGG'CCA
Cuts at: 0 310 604 948
Size: 310 294 344
Fragments arranged by size:
344 310 294
MslI CAynn'nnrTG
Cuts at: 0 172 769 948
Size: 172 597 179
Fragments arranged by size:
597 179 172
MspA1I CmG'CkG
Cuts at: 0 452 495 691 948
Size: 452 43 196 257
Fragments arranged by size:
452 257 196 43
NaeI GCC'GGC
Cuts at: 0 382 948
Size: 382 566
NcoI C'CATG_G
Cuts at: 0 311 948
Size: 311 637
NgoMIV G'CCGG_C
Cuts at: 0 380 948
Size: 380 568
PpuMI rG'GwC_Cy
Cuts at: 0 716 905 948
Size: 716 189 43
Fragments arranged by size:
716 189 43
PvuII CAG'CTG
Cuts at: 0 495 691 948
Size: 495 196 257
Fragments arranged by size:
495 257 196
SexAI A'CCwGG_T
Cuts at: 0 731 948
Size: 731 217
SmlI C'TyrA_G
Cuts at: 0 108 665 900 948
Size: 108 557 235 48
Fragments arranged by size:
557 235 108 48
SpeI A'CTAG_T
Cuts at: 0 138 948
Size: 138 810
StuI AGG'CCT
Cuts at: 0 709 948
Size: 709 239
StyI C'CwwG_G
Cuts at: 0 311 948
Size: 311 637
TatI w'GTAC_w
Cuts at: 0 95 176 948
Size: 95 81 772
Fragments arranged by size:
772 95 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 709 948
Size: 709 239
Enzymes that do cut:
AlwNI BanI BanII BbvCI BciVI BglI BlpI BmrI
BplI BpmI Bpu10I BpuEI BsaAI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BsrFI BssSI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI Eco57MI EcoO109I
Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV PpuMI
PvuII SexAI SmlI SpeI StuI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BcgI BclI BfrBI BglII Bme1580I BmgBI
BmtI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstZ17I Bsu36I ClaI DraIII EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI