(Linear) (Six-base) MAPSORT of: KIEE6032.seq  Check: 4888  from: 1  to: 948



KIEE6032.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:20  ..

AatII G_ACGT'C

Cuts at:      0     881     948 
   Size:        881      67
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     948 
   Size:        704     244
 
AflIII A'CryG_T

Cuts at:      0     563     948 
   Size:        563     385
 
AleI CACnn'nnGTG

Cuts at:      0     766     948 
   Size:        766     182
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      30     948 
   Size:         30     918
 
ApoI r'AATT_y

Cuts at:      0     114     948 
   Size:        114     834
 
AvaI C'yCGr_G

Cuts at:      0     793     948 
   Size:        793     155
 
BanI G'GyrC_C

Cuts at:      0     430     948 
   Size:        430     518
 
BanII G_rGCy'C

Cuts at:      0     448     948 
   Size:        448     500
 
BglII A'GATC_T

Cuts at:      0     290     948 
   Size:        290     658
 
BmgBI CAC'GTC

Cuts at:      0     566     948 
   Size:        566     382
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     382     771     948 
   Size:        382     389     177

  Fragments arranged by size: 

                 389     382     177
 
BsaAI yAC'GTr

Cuts at:      0     360     768     948 
   Size:        360     408     180

  Fragments arranged by size: 

                 408     360     180
 
BsaBI GATnn'nnATC

Cuts at:      0     589     948 
   Size:        589     359
 
BsaHI Gr'CG_yC

Cuts at:      0     878     948 
   Size:        878      70
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     484     514     948 
   Size:        484      30     434

  Fragments arranged by size: 

                 484     434      30
 
BseYI C'CCAG_C

Cuts at:      0     534     948 
   Size:        534     414
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     948 
   Size:        578     370
 
Bsp1286I G_dGCh'C

Cuts at:      0     448     948 
   Size:        448     500
 
BspHI T'CATG_A

Cuts at:      0     122     948 
   Size:        122     826
 
BsrGI T'GTAC_A

Cuts at:      0     587     948 
   Size:        587     361
 
BstYI r'GATC_y

Cuts at:      0     290     530     948 
   Size:        290     240     418

  Fragments arranged by size: 

                 418     290     240
 
DraI TTT'AAA

Cuts at:      0     894     948 
   Size:        894      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     948 
   Size:        704     244
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     374     406     948 
   Size:        374      32     542

  Fragments arranged by size: 

                 542     374      32
 
HincII GTy'rAC

Cuts at:      0     659     948 
   Size:        659     289
 
HpaI GTT'AAC

Cuts at:      0     659     948 
   Size:        659     289
 
MslI CAynn'nnrTG

Cuts at:      0     163     478     766     838     948 
   Size:        163     315     288      72     110

  Fragments arranged by size: 

                 315     288     163     110      72
 
MspA1I CmG'CkG

Cuts at:      0     186     948 
   Size:        186     762
 
NspI r_CATG'y

Cuts at:      0     675     911     948 
   Size:        675     236      37

  Fragments arranged by size: 

                 675     236      37
 
PvuII CAG'CTG

Cuts at:      0     186     948 
   Size:        186     762
 
SfcI C'TryA_G

Cuts at:      0     728     858     948 
   Size:        728     130      90

  Fragments arranged by size: 

                 728     130      90
 
SphI G_CATG'C

Cuts at:      0     675     948 
   Size:        675     273
 
StuI AGG'CCT

Cuts at:      0     700     948 
   Size:        700     248
 
TatI w'GTAC_w

Cuts at:      0     167     587     948 
   Size:        167     420     361

  Fragments arranged by size: 

                 420     361     167
 
ZraI GAC'GTC

Cuts at:      0     879     948 
   Size:        879      69
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AleI     AloI     ApoI     AvaI     BanI
    BanII    BglII    BmgBI     BmrI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI     BsgI Bsp1286I    BspHI    BsrGI    BstYI     DraI  Eco57MI
    Hin4I   HincII     HpaI     MslI   MspA1I     NspI    PvuII     SfcI
     SphI     StuI     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI