Restriction map for
pFN21AE6031
(Linear) (Six-base) MAPSORT of: KIEE6031.seq Check: 2782 from: 1 to: 948
KIEE6031.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AccI GT'mk_AC
Cuts at: 0 909 948
Size: 909 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 948
Size: 710 238
AflIII A'CryG_T
Cuts at: 0 572 773 912 948
Size: 572 201 139 36
Fragments arranged by size:
572 201 139 36
AhdI GACnn_n'nnGTC
Cuts at: 0 916 948
Size: 916 32
AleI CACnn'nnGTG
Cuts at: 0 22 624 948
Size: 22 602 324
Fragments arranged by size:
602 324 22
AlwNI CAG_nnn'CTG
Cuts at: 0 157 287 688 948
Size: 157 130 401 260
Fragments arranged by size:
401 260 157 130
ApoI r'AATT_y
Cuts at: 0 31 123 948
Size: 31 92 825
Fragments arranged by size:
825 92 31
BanII G_rGCy'C
Cuts at: 0 164 948
Size: 164 784
BbsI GAAGACnn'nnnn_
Cuts at: 0 591 948
Size: 591 357
BfrBI ATG'CAT
Cuts at: 0 211 948
Size: 211 737
Bme1580I G_kGCm'C
Cuts at: 0 781 948
Size: 781 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 948
Size: 44 904
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 338 948
Size: 338 610
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 948
Size: 373 575
Bsp1286I G_dGCh'C
Cuts at: 0 164 781 948
Size: 164 617 167
Fragments arranged by size:
617 167 164
BspHI T'CATG_A
Cuts at: 0 404 948
Size: 404 544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 765 948
Size: 765 183
BsrDI GCAATG_nn'
Cuts at: 0 434 948
Size: 434 514
BsrFI r'CCGG_y
Cuts at: 0 380 948
Size: 380 568
BssSI C'ACGA_G
Cuts at: 0 725 948
Size: 725 223
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 948
Size: 243 705
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 354 948
Size: 354 594
DraIII CAC_nnn'GTG
Cuts at: 0 828 948
Size: 828 120
EarI CTCTTCn'nnn_
Cuts at: 0 889 948
Size: 889 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 948
Size: 710 238
EcoO109I rG'GnC_Cy
Cuts at: 0 788 948
Size: 788 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 241 273 275 307 423 455
Size: 42 32 167 32 2 32 116 32
Cuts at: 455 536 568 746 778 798 830 902 934
Size: 81 32 178 32 20 32 72 32
Cuts at: 934 948
Size: 14
Fragments arranged by size:
178 167 116 81 72 42 32 32
32 32 32 32 32 32 20 14
2
HincII GTy'rAC
Cuts at: 0 910 948
Size: 910 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 948
Size: 567 381
MslI CAynn'nnrTG
Cuts at: 0 22 571 577 624 772 948
Size: 22 549 6 47 148 176
Fragments arranged by size:
549 176 148 47 22 6
MspA1I CmG'CkG
Cuts at: 0 519 799 948
Size: 519 280 149
Fragments arranged by size:
519 280 149
NaeI GCC'GGC
Cuts at: 0 382 948
Size: 382 566
NgoMIV G'CCGG_C
Cuts at: 0 380 948
Size: 380 568
NsiI A_TGCA'T
Cuts at: 0 213 948
Size: 213 735
NspI r_CATG'y
Cuts at: 0 576 719 777 916 948
Size: 576 143 58 139 32
Fragments arranged by size:
576 143 139 58 32
PasI CC'CwG_GG
Cuts at: 0 157 948
Size: 157 791
PciI A'CATG_T
Cuts at: 0 572 773 912 948
Size: 572 201 139 36
Fragments arranged by size:
572 201 139 36
PfoI T'CCnGG_A
Cuts at: 0 203 948
Size: 203 745
PsiI TTA'TAA
Cuts at: 0 937 948
Size: 937 11
PvuII CAG'CTG
Cuts at: 0 519 799 948
Size: 519 280 149
Fragments arranged by size:
519 280 149
SalI G'TCGA_C
Cuts at: 0 908 948
Size: 908 40
SfcI C'TryA_G
Cuts at: 0 279 948
Size: 279 669
SmlI C'TyrA_G
Cuts at: 0 59 948
Size: 59 889
StyI C'CwwG_G
Cuts at: 0 42 318 948
Size: 42 276 630
Fragments arranged by size:
630 276 42
Tth111I GACn'n_nGTC
Cuts at: 0 54 948
Size: 54 894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 474 948
Size: 243 231 474
Fragments arranged by size:
474 243 231
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlwNI ApoI BanII
BbsI BfrBI Bme1580I BpuEI BseRI BsgI Bsp1286I BspHI
BspMI BsrDI BsrFI BssSI BstXI BtgZI DraIII EarI
Eco57MI EcoO109I Hin4I HincII MmeI MslI MspA1I NaeI
NgoMIV NsiI NspI PasI PciI PfoI PsiI PvuII
SalI SfcI SmlI StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MscI
NarI NcoI NdeI NheI NotI NruI PacI PflMI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI XbaI XhoI XmaI XmnI ZraI