Restriction map for
pFN21AE6030
(Linear) (Six-base) MAPSORT of: KIEE6030.seq Check: 6228 from: 1 to: 948
KIEE6030.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 948
Size: 916 32
AflIII A'CryG_T
Cuts at: 0 724 948
Size: 724 224
AleI CACnn'nnGTG
Cuts at: 0 283 745 948
Size: 283 462 203
Fragments arranged by size:
462 283 203
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 948
Size: 149 32 767
Fragments arranged by size:
767 149 32
ApaLI G'TGCA_C
Cuts at: 0 192 948
Size: 192 756
ApoI r'AATT_y
Cuts at: 0 31 928 948
Size: 31 897 20
Fragments arranged by size:
897 31 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 163 196 713 746 948
Size: 163 33 517 33 202
Fragments arranged by size:
517 202 163 33 33
BanII G_rGCy'C
Cuts at: 0 415 948
Size: 415 533
BbsI GAAGACnn'nnnn_
Cuts at: 0 654 837 948
Size: 654 183 111
Fragments arranged by size:
654 183 111
BciVI GTATCCnnnnn_n'
Cuts at: 0 741 948
Size: 741 207
BglI GCCn_nnn'nGGC
Cuts at: 0 826 948
Size: 826 122
Bme1580I G_kGCm'C
Cuts at: 0 196 429 948
Size: 196 233 519
Fragments arranged by size:
519 233 196
Bpu10I CC'TnA_GC
Cuts at: 0 859 948
Size: 859 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 769 948
Size: 702 67 179
Fragments arranged by size:
702 179 67
BsaAI yAC'GTr
Cuts at: 0 725 948
Size: 725 223
BsaHI Gr'CG_yC
Cuts at: 0 818 948
Size: 818 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 149 179 948
Size: 149 30 769
Fragments arranged by size:
769 149 30
BseYI C'CCAG_C
Cuts at: 0 62 120 948
Size: 62 58 828
Fragments arranged by size:
828 62 58
BsiHKAI G_wGCw'C
Cuts at: 0 196 407 948
Size: 196 211 541
Fragments arranged by size:
541 211 196
BsmI GAATG_Cn'
Cuts at: 0 906 948
Size: 906 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 210 948
Size: 210 738
Bsp1286I G_dGCh'C
Cuts at: 0 196 407 415 429 948
Size: 196 211 8 14 519
Fragments arranged by size:
519 211 196 14 8
BspHI T'CATG_A
Cuts at: 0 407 948
Size: 407 541
BsrDI GCAATG_nn'
Cuts at: 0 115 948
Size: 115 833
BsrFI r'CCGG_y
Cuts at: 0 704 948
Size: 704 244
BsrGI T'GTAC_A
Cuts at: 0 809 948
Size: 809 139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 948
Size: 72 876
Bsu36I CC'TnA_GG
Cuts at: 0 880 948
Size: 880 68
BtsI GCAGTG_nn'
Cuts at: 0 388 948
Size: 388 560
EaeI y'GGCC_r
Cuts at: 0 140 374 827 948
Size: 140 234 453 121
Fragments arranged by size:
453 234 140 121
EarI CTCTTCn'nnn_
Cuts at: 0 190 948
Size: 190 758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 948
Size: 916 32
EcoO109I rG'GnC_Cy
Cuts at: 0 795 948
Size: 795 153
MscI TGG'CCA
Cuts at: 0 142 376 829 948
Size: 142 234 453 119
Fragments arranged by size:
453 234 142 119
MslI CAynn'nnrTG
Cuts at: 0 175 283 469 598 745 948
Size: 175 108 186 129 147 203
Fragments arranged by size:
203 186 175 147 129 108
NaeI GCC'GGC
Cuts at: 0 706 948
Size: 706 242
NgoMIV G'CCGG_C
Cuts at: 0 704 948
Size: 704 244
NspI r_CATG'y
Cuts at: 0 246 948
Size: 246 702
PflMI CCAn_nnn'nTGG
Cuts at: 0 149 435 948
Size: 149 286 513
Fragments arranged by size:
513 286 149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 202 234 948
Size: 149 32 21 32 714
Fragments arranged by size:
714 149 32 32 21
SfcI C'TryA_G
Cuts at: 0 15 759 948
Size: 15 744 189
Fragments arranged by size:
744 189 15
SmlI C'TyrA_G
Cuts at: 0 681 784 948
Size: 681 103 164
Fragments arranged by size:
681 164 103
SphI G_CATG'C
Cuts at: 0 246 948
Size: 246 702
StyI C'CwwG_G
Cuts at: 0 791 948
Size: 791 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 280 401 586 948
Size: 189 91 121 185 362
Fragments arranged by size:
362 189 185 121 91
TatI w'GTAC_w
Cuts at: 0 809 948
Size: 809 139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 948
Size: 282 666
Enzymes that do cut:
AcuI AflIII AleI AloI ApaLI ApoI BaeI BanII
BbsI BciVI BglI Bme1580I Bpu10I BpuEI BsaAI BsaHI
BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI
BsrFI BsrGI BstAPI Bsu36I BtsI EaeI EarI Eco57MI
EcoO109I MscI MslI NaeI NgoMIV NspI PflMI PpiI
SfcI SmlI SphI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BamHI BanI BbeI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BsaI
BsaBI BsaWI BseRI BsgI BsiEI BsiWI BspEI BspMI
BsrBI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI