Restriction map for
pFN21AE6026
(Linear) (Six-base) MAPSORT of: KIEE6026.seq Check: 1708 from: 1 to: 945
KIEE6026.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AccI GT'mk_AC
Cuts at: 0 206 945
Size: 206 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 713 945
Size: 660 53 232
Fragments arranged by size:
660 232 53
AflIII A'CryG_T
Cuts at: 0 446 718 945
Size: 446 272 227
Fragments arranged by size:
446 272 227
AleI CACnn'nnGTG
Cuts at: 0 22 945
Size: 22 923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 945
Size: 154 791
AlwNI CAG_nnn'CTG
Cuts at: 0 480 945
Size: 480 465
ApoI r'AATT_y
Cuts at: 0 31 945
Size: 31 914
BamHI G'GATC_C
Cuts at: 0 680 945
Size: 680 265
BanII G_rGCy'C
Cuts at: 0 470 847 945
Size: 470 377 98
Fragments arranged by size:
470 377 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 295 945
Size: 274 21 650
Fragments arranged by size:
650 274 21
BclI T'GATC_A
Cuts at: 0 143 323 945
Size: 143 180 622
Fragments arranged by size:
622 180 143
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 945
Size: 55 890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 945
Size: 606 339
BsaAI yAC'GTr
Cuts at: 0 719 777 945
Size: 719 58 168
Fragments arranged by size:
719 168 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 536 820 945
Size: 536 284 125
Fragments arranged by size:
536 284 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 945
Size: 229 716
Bsp1286I G_dGCh'C
Cuts at: 0 470 847 945
Size: 470 377 98
Fragments arranged by size:
470 377 98
BsrDI GCAATG_nn'
Cuts at: 0 599 945
Size: 599 346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 128 945
Size: 128 817
BstEII G'GTnAC_C
Cuts at: 0 190 945
Size: 190 755
BstYI r'GATC_y
Cuts at: 0 74 680 945
Size: 74 606 265
Fragments arranged by size:
606 265 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 297 945
Size: 297 648
BtsI GCAGTG_nn'
Cuts at: 0 478 945
Size: 478 467
DraIII CAC_nnn'GTG
Cuts at: 0 299 945
Size: 299 646
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 945
Size: 577 368
EarI CTCTTCn'nnn_
Cuts at: 0 313 637 769 945
Size: 313 324 132 176
Fragments arranged by size:
324 313 176 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 713 945
Size: 660 53 232
Fragments arranged by size:
660 232 53
EcoRI G'AATT_C
Cuts at: 0 31 945
Size: 31 914
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 875 907 945
Size: 875 32 38
Fragments arranged by size:
875 38 32
MslI CAynn'nnrTG
Cuts at: 0 22 945
Size: 22 923
NspI r_CATG'y
Cuts at: 0 450 774 945
Size: 450 324 171
Fragments arranged by size:
450 324 171
PciI A'CATG_T
Cuts at: 0 446 945
Size: 446 499
PfoI T'CCnGG_A
Cuts at: 0 400 945
Size: 400 545
PshAI GACnn'nnGTC
Cuts at: 0 739 945
Size: 739 206
SfcI C'TryA_G
Cuts at: 0 800 867 945
Size: 800 67 78
Fragments arranged by size:
800 78 67
SmlI C'TyrA_G
Cuts at: 0 621 945
Size: 621 324
SnaBI TAC'GTA
Cuts at: 0 777 945
Size: 777 168
StuI AGG'CCT
Cuts at: 0 783 945
Size: 783 162
StyI C'CwwG_G
Cuts at: 0 186 945
Size: 186 759
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 945
Size: 186 759
XmnI GAAnn'nnTTC
Cuts at: 0 35 945
Size: 35 910
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI BamHI
BanII BbsI BclI BmrI BpuEI BsaAI BseRI BsgI
Bsp1286I BsrDI BstAPI BstEII BstYI BtgZI BtsI DraIII
DrdI EarI Eco57MI EcoRI Hin4I MslI NspI PciI
PfoI PshAI SfcI SmlI SnaBI StuI StyI TaqII
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI