Restriction map for
pFN21AE6025
(Linear) (Six-base) MAPSORT of: KIEE6025.seq Check: 1212 from: 1 to: 945
KIEE6025.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 170 945
Size: 170 775
AccI GT'mk_AC
Cuts at: 0 206 945
Size: 206 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 713 945
Size: 660 53 232
Fragments arranged by size:
660 232 53
AflIII A'CryG_T
Cuts at: 0 446 718 945
Size: 446 272 227
Fragments arranged by size:
446 272 227
AleI CACnn'nnGTG
Cuts at: 0 22 945
Size: 22 923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 403 945
Size: 154 249 542
Fragments arranged by size:
542 249 154
AlwNI CAG_nnn'CTG
Cuts at: 0 480 945
Size: 480 465
ApoI r'AATT_y
Cuts at: 0 31 945
Size: 31 914
BamHI G'GATC_C
Cuts at: 0 680 945
Size: 680 265
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 295 945
Size: 274 21 650
Fragments arranged by size:
650 274 21
BclI T'GATC_A
Cuts at: 0 143 323 945
Size: 143 180 622
Fragments arranged by size:
622 180 143
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 945
Size: 55 890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 945
Size: 606 339
BsaAI yAC'GTr
Cuts at: 0 719 777 945
Size: 719 58 168
Fragments arranged by size:
719 168 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 536 820 945
Size: 536 284 125
Fragments arranged by size:
536 284 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 945
Size: 229 716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 945
Size: 170 775
BsrDI GCAATG_nn'
Cuts at: 0 599 945
Size: 599 346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 128 945
Size: 128 817
BstYI r'GATC_y
Cuts at: 0 74 680 945
Size: 74 606 265
Fragments arranged by size:
606 265 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 297 945
Size: 297 648
BtsI GCAGTG_nn'
Cuts at: 0 478 945
Size: 478 467
DraIII CAC_nnn'GTG
Cuts at: 0 299 945
Size: 299 646
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 945
Size: 577 368
EaeI y'GGCC_r
Cuts at: 0 189 945
Size: 189 756
EarI CTCTTCn'nnn_
Cuts at: 0 313 637 769 945
Size: 313 324 132 176
Fragments arranged by size:
324 313 176 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 713 945
Size: 660 53 232
Fragments arranged by size:
660 232 53
EcoRI G'AATT_C
Cuts at: 0 31 945
Size: 31 914
MscI TGG'CCA
Cuts at: 0 191 945
Size: 191 754
MslI CAynn'nnrTG
Cuts at: 0 22 945
Size: 22 923
NspI r_CATG'y
Cuts at: 0 450 774 945
Size: 450 324 171
Fragments arranged by size:
450 324 171
PciI A'CATG_T
Cuts at: 0 446 945
Size: 446 499
PfoI T'CCnGG_A
Cuts at: 0 400 945
Size: 400 545
PshAI GACnn'nnGTC
Cuts at: 0 739 945
Size: 739 206
PstI C_TGCA'G
Cuts at: 0 825 945
Size: 825 120
SfcI C'TryA_G
Cuts at: 0 800 821 867 945
Size: 800 21 46 78
Fragments arranged by size:
800 78 46 21
SmlI C'TyrA_G
Cuts at: 0 621 945
Size: 621 324
SnaBI TAC'GTA
Cuts at: 0 777 945
Size: 777 168
StuI AGG'CCT
Cuts at: 0 783 945
Size: 783 162
StyI C'CwwG_G
Cuts at: 0 186 945
Size: 186 759
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 945
Size: 186 759
XmnI GAAnn'nnTTC
Cuts at: 0 35 945
Size: 35 910
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApoI
BamHI BbsI BclI BmrI BpuEI BsaAI BseRI BsgI
BspMI BsrDI BstAPI BstYI BtgZI BtsI DraIII DrdI
EaeI EarI Eco57MI EcoRI MscI MslI NspI PciI
PfoI PshAI PstI SfcI SmlI SnaBI StuI StyI
TaqII XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI