Restriction map for
pFN21AE6024
(Linear) (Six-base) MAPSORT of: KIEE6024.seq Check: 4802 from: 1 to: 945
KIEE6024.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 663 925 945
Size: 55 608 262 20
Fragments arranged by size:
608 262 55 20
AleI CACnn'nnGTG
Cuts at: 0 580 841 945
Size: 580 261 104
Fragments arranged by size:
580 261 104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 835 945
Size: 835 110
AlwNI CAG_nnn'CTG
Cuts at: 0 610 945
Size: 610 335
ApoI r'AATT_y
Cuts at: 0 31 945
Size: 31 914
BamHI G'GATC_C
Cuts at: 0 649 945
Size: 649 296
BanII G_rGCy'C
Cuts at: 0 934 945
Size: 934 11
BbvCI CC'TCA_GC
Cuts at: 0 695 945
Size: 695 250
BclI T'GATC_A
Cuts at: 0 257 945
Size: 257 688
BglI GCCn_nnn'nGGC
Cuts at: 0 355 433 945
Size: 355 78 512
Fragments arranged by size:
512 355 78
Bme1580I G_kGCm'C
Cuts at: 0 239 429 945
Size: 239 190 516
Fragments arranged by size:
516 239 190
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 945
Size: 394 551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 613 945
Size: 229 384 332
Fragments arranged by size:
384 332 229
Bpu10I CC'TnA_GC
Cuts at: 0 695 945
Size: 695 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 861 945
Size: 861 84
BsaAI yAC'GTr
Cuts at: 0 372 776 945
Size: 372 404 169
Fragments arranged by size:
404 372 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 71 101 945
Size: 71 30 844
Fragments arranged by size:
844 71 30
BseYI C'CCAG_C
Cuts at: 0 246 428 945
Size: 246 182 517
Fragments arranged by size:
517 246 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 884 945
Size: 146 738 61
Fragments arranged by size:
738 146 61
BsmI GAATG_Cn'
Cuts at: 0 712 945
Size: 712 233
Bsp1286I G_dGCh'C
Cuts at: 0 239 429 934 945
Size: 239 190 505 11
Fragments arranged by size:
505 239 190 11
BsrDI GCAATG_nn'
Cuts at: 0 132 945
Size: 132 813
BssSI C'ACGA_G
Cuts at: 0 40 945
Size: 40 905
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 246 945
Size: 108 138 699
Fragments arranged by size:
699 138 108
BstYI r'GATC_y
Cuts at: 0 649 945
Size: 649 296
BtgI C'CryG_G
Cuts at: 0 353 945
Size: 353 592
BtsI GCAGTG_nn'
Cuts at: 0 761 945
Size: 761 184
EaeI y'GGCC_r
Cuts at: 0 347 374 467 945
Size: 347 27 93 478
Fragments arranged by size:
478 347 93 27
EarI CTCTTCn'nnn_
Cuts at: 0 757 807 837 945
Size: 757 50 30 108
Fragments arranged by size:
757 108 50 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 717 945
Size: 717 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 229 613 663 925 945
Size: 55 174 384 50 262 20
Fragments arranged by size:
384 262 174 55 50 20
EcoO109I rG'GnC_Cy
Cuts at: 0 251 923 945
Size: 251 672 22
Fragments arranged by size:
672 251 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 641 673 945
Size: 641 32 272
Fragments arranged by size:
641 272 32
MfeI C'AATT_G
Cuts at: 0 20 945
Size: 20 925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 945
Size: 807 138
MscI TGG'CCA
Cuts at: 0 349 376 469 945
Size: 349 27 93 476
Fragments arranged by size:
476 349 93 27
MslI CAynn'nnrTG
Cuts at: 0 301 580 841 945
Size: 301 279 261 104
Fragments arranged by size:
301 279 261 104
MspA1I CmG'CkG
Cuts at: 0 432 439 694 945
Size: 432 7 255 251
Fragments arranged by size:
432 255 251 7
NcoI C'CATG_G
Cuts at: 0 353 945
Size: 353 592
NdeI CA'TA_TG
Cuts at: 0 360 945
Size: 360 585
PasI CC'CwG_GG
Cuts at: 0 927 945
Size: 927 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 738 945
Size: 738 207
PfoI T'CCnGG_A
Cuts at: 0 206 945
Size: 206 739
PpuMI rG'GwC_Cy
Cuts at: 0 251 923 945
Size: 251 672 22
Fragments arranged by size:
672 251 22
PshAI GACnn'nnGTC
Cuts at: 0 22 945
Size: 22 923
PvuII CAG'CTG
Cuts at: 0 432 439 694 945
Size: 432 7 255 251
Fragments arranged by size:
432 255 251 7
SanDI GG'GwC_CC
Cuts at: 0 251 945
Size: 251 694
SfcI C'TryA_G
Cuts at: 0 149 756 870 945
Size: 149 607 114 75
Fragments arranged by size:
607 149 114 75
SmlI C'TyrA_G
Cuts at: 0 876 945
Size: 876 69
StyI C'CwwG_G
Cuts at: 0 285 353 945
Size: 285 68 592
Fragments arranged by size:
592 285 68
TatI w'GTAC_w
Cuts at: 0 179 945
Size: 179 766
XmnI GAAnn'nnTTC
Cuts at: 0 35 529 945
Size: 35 494 416
Fragments arranged by size:
494 416 35
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApoI BamHI BanII BbvCI
BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI
BsaXI BseYI BsgI BsmI Bsp1286I BsrDI BssSI BstXI
BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I
Hin4I MfeI MmeI MscI MslI MspA1I NcoI NdeI
PasI PflMI PfoI PpuMI PshAI PvuII SanDI SfcI
SmlI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BcgI BciVI
BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaBI
BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI