Restriction map for
pFN21AE6023
(Linear) (Six-base) MAPSORT of: KIEE6023.seq Check: 2368 from: 1 to: 945
KIEE6023.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 710 945
Size: 43 667 235
Fragments arranged by size:
667 235 43
AflIII A'CryG_T
Cuts at: 0 572 945
Size: 572 373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 459 945
Size: 459 486
AlwNI CAG_nnn'CTG
Cuts at: 0 28 945
Size: 28 917
ApaI G_GGCC'C
Cuts at: 0 512 945
Size: 512 433
ApoI r'AATT_y
Cuts at: 0 31 945
Size: 31 914
AseI AT'TA_AT
Cuts at: 0 130 945
Size: 130 815
AvaI C'yCGr_G
Cuts at: 0 472 945
Size: 472 473
BanII G_rGCy'C
Cuts at: 0 512 945
Size: 512 433
BbsI GAAGACnn'nnnn_
Cuts at: 0 729 907 945
Size: 729 178 38
Fragments arranged by size:
729 178 38
BbvCI CC'TCA_GC
Cuts at: 0 53 945
Size: 53 892
BclI T'GATC_A
Cuts at: 0 401 945
Size: 401 544
BglII A'GATC_T
Cuts at: 0 209 527 945
Size: 209 318 418
Fragments arranged by size:
418 318 209
BlpI GC'TnA_GC
Cuts at: 0 553 945
Size: 553 392
Bme1580I G_kGCm'C
Cuts at: 0 512 945
Size: 512 433
BmgBI CAC'GTC
Cuts at: 0 575 945
Size: 575 370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 945
Size: 226 719
Bpu10I CC'TnA_GC
Cuts at: 0 53 945
Size: 53 892
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 945
Size: 199 30 716
Fragments arranged by size:
716 199 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 945
Size: 740 205
BsiHKAI G_wGCw'C
Cuts at: 0 426 945
Size: 426 519
Bsp1286I G_dGCh'C
Cuts at: 0 426 512 945
Size: 426 86 433
Fragments arranged by size:
433 426 86
BspHI T'CATG_A
Cuts at: 0 404 945
Size: 404 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 945
Size: 427 518
BsrBI CCG'CTC
Cuts at: 0 8 945
Size: 8 937
BsrDI GCAATG_nn'
Cuts at: 0 635 815 945
Size: 635 180 130
Fragments arranged by size:
635 180 130
BsrGI T'GTAC_A
Cuts at: 0 565 945
Size: 565 380
BstYI r'GATC_y
Cuts at: 0 209 335 527 945
Size: 209 126 192 418
Fragments arranged by size:
418 209 192 126
BtgI C'CryG_G
Cuts at: 0 350 945
Size: 350 595
BtsI GCAGTG_nn'
Cuts at: 0 493 762 945
Size: 493 269 183
Fragments arranged by size:
493 269 183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 785 820 945
Size: 785 35 125
Fragments arranged by size:
785 125 35
EaeI y'GGCC_r
Cuts at: 0 353 945
Size: 353 592
EarI CTCTTCn'nnn_
Cuts at: 0 392 454 690 945
Size: 392 62 236 255
Fragments arranged by size:
392 255 236 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 226 710 945
Size: 43 183 484 235
Fragments arranged by size:
484 235 183 43
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 945
Size: 272 673
MscI TGG'CCA
Cuts at: 0 355 945
Size: 355 590
MslI CAynn'nnrTG
Cuts at: 0 277 577 945
Size: 277 300 368
Fragments arranged by size:
368 300 277
NcoI C'CATG_G
Cuts at: 0 350 945
Size: 350 595
PflMI CCAn_nnn'nTGG
Cuts at: 0 732 945
Size: 732 213
PspOMI G'GGCC_C
Cuts at: 0 508 945
Size: 508 437
PstI C_TGCA'G
Cuts at: 0 438 945
Size: 438 507
SfcI C'TryA_G
Cuts at: 0 434 945
Size: 434 511
StyI C'CwwG_G
Cuts at: 0 350 945
Size: 350 595
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 172 945
Size: 172 773
TatI w'GTAC_w
Cuts at: 0 77 565 770 834 945
Size: 77 488 205 64 111
Fragments arranged by size:
488 205 111 77 64
Tth111I GACn'n_nGTC
Cuts at: 0 541 945
Size: 541 404
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI AseI AvaI
BanII BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BsaXI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BstYI BtgI BtsI CspCI EaeI
EarI Eco57MI MmeI MscI MslI NcoI PflMI PspOMI
PstI SfcI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BfrBI BglI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI XmaI XmnI ZraI