Restriction map for
pFN21AE6018
(Linear) (Six-base) MAPSORT of: KIEE6018.seq Check: 958 from: 1 to: 945
KIEE6018.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 730 945
Size: 730 215
AccI GT'mk_AC
Cuts at: 0 572 945
Size: 572 373
ApaI G_GGCC'C
Cuts at: 0 804 945
Size: 804 141
ApoI r'AATT_y
Cuts at: 0 495 945
Size: 495 450
AvrII C'CTAG_G
Cuts at: 0 787 945
Size: 787 158
BanII G_rGCy'C
Cuts at: 0 341 445 804 945
Size: 341 104 359 141
Fragments arranged by size:
359 341 141 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 96 606 945
Size: 96 510 339
Fragments arranged by size:
510 339 96
BbvCI CC'TCA_GC
Cuts at: 0 190 945
Size: 190 755
Bme1580I G_kGCm'C
Cuts at: 0 804 903 945
Size: 804 99 42
Fragments arranged by size:
804 99 42
BmgBI CAC'GTC
Cuts at: 0 8 945
Size: 8 937
BmrI ACTGGGnnnn_n'
Cuts at: 0 446 945
Size: 446 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 945
Size: 412 533
Bpu10I CC'TnA_GC
Cuts at: 0 190 695 945
Size: 190 505 250
Fragments arranged by size:
505 250 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 897 945
Size: 534 363 48
Fragments arranged by size:
534 363 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 717 945
Size: 124 593 228
Fragments arranged by size:
593 228 124
BseYI C'CCAG_C
Cuts at: 0 345 805 945
Size: 345 460 140
Fragments arranged by size:
460 345 140
BsiHKAI G_wGCw'C
Cuts at: 0 445 945
Size: 445 500
Bsp1286I G_dGCh'C
Cuts at: 0 341 445 804 903 945
Size: 341 104 359 99 42
Fragments arranged by size:
359 341 104 99 42
BspHI T'CATG_A
Cuts at: 0 407 945
Size: 407 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 730 945
Size: 730 215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 436 645 945
Size: 436 209 300
Fragments arranged by size:
436 300 209
BstYI r'GATC_y
Cuts at: 0 328 945
Size: 328 617
Bsu36I CC'TnA_GG
Cuts at: 0 781 945
Size: 781 164
BtgI C'CryG_G
Cuts at: 0 89 945
Size: 89 856
ClaI AT'CG_AT
Cuts at: 0 464 945
Size: 464 481
EaeI y'GGCC_r
Cuts at: 0 374 716 945
Size: 374 342 229
Fragments arranged by size:
374 342 229
EarI CTCTTCn'nnn_
Cuts at: 0 88 631 757 945
Size: 88 543 126 188
Fragments arranged by size:
543 188 126 88
EcoICRI GAG'CTC
Cuts at: 0 443 945
Size: 443 502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 945
Size: 412 533
EcoO109I rG'GnC_Cy
Cuts at: 0 711 801 945
Size: 711 90 144
Fragments arranged by size:
711 144 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 408 440 532 564 945
Size: 408 32 92 32 381
Fragments arranged by size:
408 381 92 32 32
MscI TGG'CCA
Cuts at: 0 376 718 945
Size: 376 342 227
Fragments arranged by size:
376 342 227
MslI CAynn'nnrTG
Cuts at: 0 151 175 406 643 945
Size: 151 24 231 237 302
Fragments arranged by size:
302 237 231 151 24
MspA1I CmG'CkG
Cuts at: 0 742 923 945
Size: 742 181 22
Fragments arranged by size:
742 181 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 945
Size: 483 462
PshAI GACnn'nnGTC
Cuts at: 0 742 945
Size: 742 203
PspOMI G'GGCC_C
Cuts at: 0 800 945
Size: 800 145
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 7 39 945
Size: 7 32 906
Fragments arranged by size:
906 32 7
PstI C_TGCA'G
Cuts at: 0 928 945
Size: 928 17
PvuII CAG'CTG
Cuts at: 0 742 945
Size: 742 203
SacI G_AGCT'C
Cuts at: 0 445 945
Size: 445 500
ScaI AGT'ACT
Cuts at: 0 66 945
Size: 66 879
SfcI C'TryA_G
Cuts at: 0 924 945
Size: 924 21
SmlI C'TyrA_G
Cuts at: 0 513 876 945
Size: 513 363 69
Fragments arranged by size:
513 363 69
StuI AGG'CCT
Cuts at: 0 700 786 945
Size: 700 86 159
Fragments arranged by size:
700 159 86
StyI C'CwwG_G
Cuts at: 0 787 945
Size: 787 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 401 945
Size: 401 544
TatI w'GTAC_w
Cuts at: 0 64 179 945
Size: 64 115 766
Fragments arranged by size:
766 115 64
Tth111I GACn'n_nGTC
Cuts at: 0 76 945
Size: 76 869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 945
Size: 282 663
XmnI GAAnn'nnTTC
Cuts at: 0 529 945
Size: 529 416
Enzymes that do cut:
AarI AccI ApaI ApoI AvrII BanII BbsI BbvCI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BseRI BseYI
BsiHKAI Bsp1286I BspHI BspMI BstXI BstYI Bsu36I BtgI
ClaI EaeI EarI EcoICRI Eco57MI EcoO109I Hin4I MscI
MslI MspA1I PflMI PshAI PspOMI PsrI PstI PvuII
SacI ScaI SfcI SmlI StuI StyI TaqII TatI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI XbaI XhoI XmaI
ZraI