Restriction map for
pFN21AE6016
(Linear) (Six-base) MAPSORT of: KIEE6016.seq Check: 9656 from: 1 to: 945
KIEE6016.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 706 945
Size: 680 26 239
Fragments arranged by size:
680 239 26
AflIII A'CryG_T
Cuts at: 0 725 727 945
Size: 725 2 218
Fragments arranged by size:
725 218 2
ApoI r'AATT_y
Cuts at: 0 603 945
Size: 603 342
BanII G_rGCy'C
Cuts at: 0 475 945
Size: 475 470
BbsI GAAGACnn'nnnn_
Cuts at: 0 283 945
Size: 283 662
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 392 945
Size: 358 34 553
Fragments arranged by size:
553 358 34
BclI T'GATC_A
Cuts at: 0 152 945
Size: 152 793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 372 783 945
Size: 61 311 411 162
Fragments arranged by size:
411 311 162 61
Bpu10I CC'TnA_GC
Cuts at: 0 196 469 945
Size: 196 273 476
Fragments arranged by size:
476 273 196
BsaAI yAC'GTr
Cuts at: 0 728 945
Size: 728 217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 337 945
Size: 76 261 608
Fragments arranged by size:
608 261 76
BseYI C'CCAG_C
Cuts at: 0 793 945
Size: 793 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 945
Size: 157 788
BsiEI CG_ry'CG
Cuts at: 0 372 945
Size: 372 573
BsiHKAI G_wGCw'C
Cuts at: 0 475 945
Size: 475 470
Bsp1286I G_dGCh'C
Cuts at: 0 475 945
Size: 475 470
BspHI T'CATG_A
Cuts at: 0 413 945
Size: 413 532
BsrGI T'GTAC_A
Cuts at: 0 430 945
Size: 430 515
BssSI C'ACGA_G
Cuts at: 0 610 945
Size: 610 335
BstEII G'GTnAC_C
Cuts at: 0 618 945
Size: 618 327
BstYI r'GATC_y
Cuts at: 0 689 945
Size: 689 256
EarI CTCTTCn'nnn_
Cuts at: 0 234 945
Size: 234 711
EcoICRI GAG'CTC
Cuts at: 0 473 945
Size: 473 472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 372 680 706 783 945
Size: 61 311 308 26 77 162
Fragments arranged by size:
311 308 162 77 61 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 913 945
Size: 481 432 32
Fragments arranged by size:
481 432 32
EcoO109I rG'GnC_Cy
Cuts at: 0 81 423 945
Size: 81 342 522
Fragments arranged by size:
522 342 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 509 541 945
Size: 509 32 404
Fragments arranged by size:
509 404 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 669 902 945
Size: 606 63 233 43
Fragments arranged by size:
606 233 63 43
MslI CAynn'nnrTG
Cuts at: 0 181 412 945
Size: 181 231 533
Fragments arranged by size:
533 231 181
NdeI CA'TA_TG
Cuts at: 0 453 945
Size: 453 492
PasI CC'CwG_GG
Cuts at: 0 420 945
Size: 420 525
PflMI CCAn_nnn'nTGG
Cuts at: 0 17 945
Size: 17 928
PmlI CAC'GTG
Cuts at: 0 728 945
Size: 728 217
SacI G_AGCT'C
Cuts at: 0 475 945
Size: 475 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 195 386 945
Size: 181 14 191 559
Fragments arranged by size:
559 191 181 14
TatI w'GTAC_w
Cuts at: 0 185 430 782 945
Size: 185 245 352 163
Fragments arranged by size:
352 245 185 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 51 83 895 927 945
Size: 51 32 812 32 18
Fragments arranged by size:
812 51 32 32 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 945
Size: 288 657
Enzymes that do cut:
AcuI AflIII ApoI BanII BbsI BcgI BclI BpmI
Bpu10I BsaAI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BsrGI BssSI BstEII BstYI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I MmeI MslI NdeI PasI PflMI
PmlI SacI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI