(Linear) (Six-base) MAPSORT of: KIEE6015.seq  Check: 7248  from: 1  to: 945



KIEE6015.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     727     945 
   Size:        727     218
 
AleI CACnn'nnGTG

Cuts at:      0     838     945 
   Size:        838     107
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     579     945 
   Size:        579     366
 
ApoI r'AATT_y

Cuts at:      0      12     615     907     945 
   Size:         12     603     292      38

  Fragments arranged by size: 

                 603     292      38      12
 
BanII G_rGCy'C

Cuts at:      0      68     945 
   Size:         68     877
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      81     945 
   Size:         81     864
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     674     798     945 
   Size:        674     124     147

  Fragments arranged by size: 

                 674     147     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     310     945 
   Size:        310     635
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     945 
   Size:         52     893
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     101     945 
   Size:         68      33     844

  Fragments arranged by size: 

                 844      68      33
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     101     945 
   Size:         68      33     844

  Fragments arranged by size: 

                 844      68      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     727     945 
   Size:        727     218
 
BsrDI GCAATG_nn'

Cuts at:      0     227     945 
   Size:        227     718
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     300     945 
   Size:        300     645
 
Bsu36I CC'TnA_GG

Cuts at:      0     874     945 
   Size:        874      71
 
BtgI C'CryG_G

Cuts at:      0     113     945 
   Size:        113     832
 
DraI TTT'AAA

Cuts at:      0     936     945 
   Size:        936       9
 
EaeI y'GGCC_r

Cuts at:      0     371     945 
   Size:        371     574
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     513     945 
   Size:         85     428     432

  Fragments arranged by size: 

                 432     428      85
 
EcoICRI GAG'CTC

Cuts at:      0      66     945 
   Size:         66     879
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     945 
   Size:         52     893
 
HincII GTy'rAC

Cuts at:      0     153     945 
   Size:        153     792
 
MscI TGG'CCA

Cuts at:      0     373     945 
   Size:        373     572
 
MslI CAynn'nnrTG

Cuts at:      0     400     838     945 
   Size:        400     438     107

  Fragments arranged by size: 

                 438     400     107
 
NcoI C'CATG_G

Cuts at:      0     113     945 
   Size:        113     832
 
NdeI CA'TA_TG

Cuts at:      0     731     945 
   Size:        731     214
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     801     945 
   Size:        801     144
 
PstI C_TGCA'G

Cuts at:      0     609     945 
   Size:        609     336
 
SacI G_AGCT'C

Cuts at:      0      68     945 
   Size:         68     877
 
SapI GCTCTTCn'nnn_

Cuts at:      0     513     945 
   Size:        513     432
 
SfcI C'TryA_G

Cuts at:      0     461     605     867     945 
   Size:        461     144     262      78

  Fragments arranged by size: 

                 461     262     144      78
 
SspI AAT'ATT

Cuts at:      0     660     945 
   Size:        660     285
 
StyI C'CwwG_G

Cuts at:      0     113     945 
   Size:        113     832
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     310     945 
   Size:        310     635
 
TatI w'GTAC_w

Cuts at:      0     176     945 
   Size:        176     769
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     798     945 
   Size:        798     147
 

 Enzymes that do cut:

     AarI     AleI     AlfI     ApoI    BanII     BbsI    BciVI     BmrI
     BpmI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstXI   Bsu36I     BtgI
     DraI     EaeI     EarI  EcoICRI  Eco57MI   HincII     MscI     MslI
     NcoI     NdeI    PflMI     PstI     SacI     SapI     SfcI     SspI
     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI