(Linear) (Six-base) MAPSORT of: KIEE6014.seq  Check: 6553  from: 1  to: 942



KIEE6014.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     427     942 
   Size:        427     515
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     942 
   Size:         77     865
 
ApoI r'AATT_y

Cuts at:      0      28     942 
   Size:         28     914
 
AseI AT'TA_AT

Cuts at:      0     112     942 
   Size:        112     830
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     942 
   Size:        223     719
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661     942 
   Size:        661     281
 
BsaAI yAC'GTr

Cuts at:      0     777     942 
   Size:        777     165
 
BsaWI w'CCGG_w

Cuts at:      0     875     942 
   Size:        875      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     649     679     942 
   Size:        649      30     263

  Fragments arranged by size: 

                 649     263      30
 
BspEI T'CCGG_A

Cuts at:      0     875     942 
   Size:        875      67
 
BspHI T'CATG_A

Cuts at:      0     134     942 
   Size:        134     808
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     427     942 
   Size:        427     515
 
BsrDI GCAATG_nn'

Cuts at:      0     356     942 
   Size:        356     586
 
BsrFI r'CCGG_y

Cuts at:      0     474     942 
   Size:        474     468
 
BstYI r'GATC_y

Cuts at:      0     206     680     942 
   Size:        206     474     262

  Fragments arranged by size: 

                 474     262     206
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     338     373     942 
   Size:        338      35     569

  Fragments arranged by size: 

                 569     338      35
 
EaeI y'GGCC_r

Cuts at:      0     186     472     696     942 
   Size:        186     286     224     246

  Fragments arranged by size: 

                 286     246     224     186
 
EarI CTCTTCn'nnn_

Cuts at:      0     307     601     942 
   Size:        307     294     341

  Fragments arranged by size: 

                 341     307     294
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     223     942 
   Size:         77     146     719

  Fragments arranged by size: 

                 719     146      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     897     942 
   Size:        897      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     239     271     942 
   Size:        239      32     671

  Fragments arranged by size: 

                 671     239      32
 
HincII GTy'rAC

Cuts at:      0     928     942 
   Size:        928      14
 
MfeI C'AATT_G

Cuts at:      0     547     641     810     942 
   Size:        547      94     169     132

  Fragments arranged by size: 

                 547     169     132      94
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     674     942 
   Size:        674     268
 
MslI CAynn'nnrTG

Cuts at:      0     133     403     942 
   Size:        133     270     539

  Fragments arranged by size: 

                 539     270     133
 
MspA1I CmG'CkG

Cuts at:      0     761     802     942 
   Size:        761      41     140

  Fragments arranged by size: 

                 761     140      41
 
PasI CC'CwG_GG

Cuts at:      0     259     942 
   Size:        259     683
 
PstI C_TGCA'G

Cuts at:      0     164     942 
   Size:        164     778
 
PvuII CAG'CTG

Cuts at:      0     761     802     942 
   Size:        761      41     140

  Fragments arranged by size: 

                 761     140      41
 
SapI GCTCTTCn'nnn_

Cuts at:      0     307     942 
   Size:        307     635
 
SfcI C'TryA_G

Cuts at:      0     160     942 
   Size:        160     782
 
SmlI C'TyrA_G

Cuts at:      0     676     942 
   Size:        676     266
 
StyI C'CwwG_G

Cuts at:      0      39     942 
   Size:         39     903
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     435     554     942 
   Size:        435     119     388

  Fragments arranged by size: 

                 435     388     119
 
XmnI GAAnn'nnTTC

Cuts at:      0     709     942 
   Size:        709     233
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     AseI     BpmI    BpuEI    BsaAI    BsaWI
    BsaXI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BstYI    CspCI
     EaeI     EarI  Eco57MI EcoO109I    Hin4I   HincII     MfeI     MmeI
     MslI   MspA1I     PasI     PstI    PvuII     SapI     SfcI     SmlI
     StyI    TaqII     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaBI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BsrBI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI