Restriction map for
pFN21AE6012
(Linear) (Six-base) MAPSORT of: KIEE6012.seq Check: 5865 from: 1 to: 942
KIEE6012.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 733 942
Size: 733 209
AccI GT'mk_AC
Cuts at: 0 842 942
Size: 842 100
AleI CACnn'nnGTG
Cuts at: 0 148 740 942
Size: 148 592 202
Fragments arranged by size:
592 202 148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 942
Size: 541 401
BanI G'GyrC_C
Cuts at: 0 16 942
Size: 16 926
BanII G_rGCy'C
Cuts at: 0 605 942
Size: 605 337
BbvCI CC'TCA_GC
Cuts at: 0 133 942
Size: 133 809
BciVI GTATCCnnnnn_n'
Cuts at: 0 56 942
Size: 56 886
BclI T'GATC_A
Cuts at: 0 611 942
Size: 611 331
BfrBI ATG'CAT
Cuts at: 0 661 942
Size: 661 281
BglII A'GATC_T
Cuts at: 0 552 942
Size: 552 390
Bme1580I G_kGCm'C
Cuts at: 0 767 942
Size: 767 175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 942
Size: 25 917
Bpu10I CC'TnA_GC
Cuts at: 0 133 942
Size: 133 809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 901 942
Size: 694 207 41
Fragments arranged by size:
694 207 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 942
Size: 13 929
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 100 130 310 340 942
Size: 100 30 180 30 602
Fragments arranged by size:
602 180 100 30 30
BseYI C'CCAG_C
Cuts at: 0 3 942
Size: 3 939
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 942
Size: 764 178
BsiHKAI G_wGCw'C
Cuts at: 0 413 810 942
Size: 413 397 132
Fragments arranged by size:
413 397 132
BsmI GAATG_Cn'
Cuts at: 0 53 137 942
Size: 53 84 805
Fragments arranged by size:
805 84 53
Bsp1286I G_dGCh'C
Cuts at: 0 413 605 767 810 942
Size: 413 192 162 43 132
Fragments arranged by size:
413 192 162 132 43
BspHI T'CATG_A
Cuts at: 0 557 827 942
Size: 557 270 115
Fragments arranged by size:
557 270 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 942
Size: 733 209
BsrDI GCAATG_nn'
Cuts at: 0 525 942
Size: 525 417
BstEII G'GTnAC_C
Cuts at: 0 127 942
Size: 127 815
BstXI CCAn_nnnn'nTGG
Cuts at: 0 363 942
Size: 363 579
BstYI r'GATC_y
Cuts at: 0 552 899 942
Size: 552 347 43
Fragments arranged by size:
552 347 43
BtsI GCAGTG_nn'
Cuts at: 0 752 942
Size: 752 190
EaeI y'GGCC_r
Cuts at: 0 353 942
Size: 353 589
EarI CTCTTCn'nnn_
Cuts at: 0 478 942
Size: 478 464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 942
Size: 25 917
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 942
Size: 351 32 559
Fragments arranged by size:
559 351 32
HincII GTy'rAC
Cuts at: 0 588 942
Size: 588 354
HpaI GTT'AAC
Cuts at: 0 588 942
Size: 588 354
MscI TGG'CCA
Cuts at: 0 355 942
Size: 355 587
MslI CAynn'nnrTG
Cuts at: 0 148 727 740 942
Size: 148 579 13 202
Fragments arranged by size:
579 202 148 13
MspA1I CmG'CkG
Cuts at: 0 45 705 942
Size: 45 660 237
Fragments arranged by size:
660 237 45
NsiI A_TGCA'T
Cuts at: 0 663 942
Size: 663 279
NspI r_CATG'y
Cuts at: 0 572 732 942
Size: 572 160 210
Fragments arranged by size:
572 210 160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 692 724 942
Size: 692 32 218
Fragments arranged by size:
692 218 32
PvuII CAG'CTG
Cuts at: 0 45 942
Size: 45 897
SfcI C'TryA_G
Cuts at: 0 879 942
Size: 879 63
SmlI C'TyrA_G
Cuts at: 0 709 916 942
Size: 709 207 26
Fragments arranged by size:
709 207 26
StuI AGG'CCT
Cuts at: 0 74 942
Size: 74 868
StyI C'CwwG_G
Cuts at: 0 893 942
Size: 893 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 873 942
Size: 873 69
TatI w'GTAC_w
Cuts at: 0 182 942
Size: 182 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 942
Size: 310 32 600
Fragments arranged by size:
600 310 32
Enzymes that do cut:
AarI AccI AleI AlfI BanI BanII BbvCI BciVI
BclI BfrBI BglII Bme1580I BpmI Bpu10I BpuEI BsaI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BstEII BstXI BstYI BtsI EaeI EarI Eco57MI
Hin4I HincII HpaI MscI MslI MspA1I NsiI NspI
PpiI PvuII SfcI SmlI StuI StyI TaqII TatI
TstI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BglI BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI