(Linear) (Six-base) MAPSORT of: KIEE6011.seq  Check: 7372  from: 1  to: 942



KIEE6011.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:19  ..

AccI GT'mk_AC

Cuts at:      0     107     400     942 
   Size:        107     293     542

  Fragments arranged by size: 

                 542     293     107
 
AflIII A'CryG_T

Cuts at:      0     902     942 
   Size:        902      40
 
AleI CACnn'nnGTG

Cuts at:      0     121     942 
   Size:        121     821
 
AlwNI CAG_nnn'CTG

Cuts at:      0     118     942 
   Size:        118     824
 
BanI G'GyrC_C

Cuts at:      0     704     942 
   Size:        704     238
 
BbeI G_GCGC'C

Cuts at:      0     708     942 
   Size:        708     234
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     837     871     942 
   Size:        837      34      71

  Fragments arranged by size: 

                 837      71      34
 
BsaBI GATnn'nnATC

Cuts at:      0     145     942 
   Size:        145     797
 
BsaHI Gr'CG_yC

Cuts at:      0     705     942 
   Size:        705     237
 
BseYI C'CCAG_C

Cuts at:      0     442     942 
   Size:        442     500
 
BsiHKAI G_wGCw'C

Cuts at:      0     432     472     942 
   Size:        432      40     470

  Fragments arranged by size: 

                 470     432      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     432     472     942 
   Size:        432      40     470

  Fragments arranged by size: 

                 470     432      40
 
BstZ17I GTA'TAC

Cuts at:      0     401     942 
   Size:        401     541
 
Bsu36I CC'TnA_GG

Cuts at:      0     880     942 
   Size:        880      62
 
BtgI C'CryG_G

Cuts at:      0     759     942 
   Size:        759     183
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     370     942 
   Size:        370     572
 
EarI CTCTTCn'nnn_

Cuts at:      0     437     942 
   Size:        437     505
 
EcoO109I rG'GnC_Cy

Cuts at:      0     446     942 
   Size:        446     496
 
EcoRV GAT'ATC

Cuts at:      0     894     942 
   Size:        894      48
 
HaeII r_GCGC'y

Cuts at:      0     708     942 
   Size:        708     234
 
KasI G'GCGC_C

Cuts at:      0     704     942 
   Size:        704     238
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     942 
   Size:        230     712
 
MslI CAynn'nnrTG

Cuts at:      0     121     409     942 
   Size:        121     288     533

  Fragments arranged by size: 

                 533     288     121
 
NarI GG'CG_CC

Cuts at:      0     705     942 
   Size:        705     237
 
NcoI C'CATG_G

Cuts at:      0     759     942 
   Size:        759     183
 
NspI r_CATG'y

Cuts at:      0     906     942 
   Size:        906      36
 
PciI A'CATG_T

Cuts at:      0     902     942 
   Size:        902      40
 
PpuMI rG'GwC_Cy

Cuts at:      0     446     942 
   Size:        446     496
 
SapI GCTCTTCn'nnn_

Cuts at:      0     437     942 
   Size:        437     505
 
SfcI C'TryA_G

Cuts at:      0     119     230     873     942 
   Size:        119     111     643      69

  Fragments arranged by size: 

                 643     119     111      69
 
SfoI GGC'GCC

Cuts at:      0     706     942 
   Size:        706     236
 
StyI C'CwwG_G

Cuts at:      0     759     942 
   Size:        759     183
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      48     942 
   Size:         48     894
 
TatI w'GTAC_w

Cuts at:      0     182     942 
   Size:        182     760
 
XmnI GAAnn'nnTTC

Cuts at:      0     715     942 
   Size:        715     227
 

 Enzymes that do cut:

     AccI   AflIII     AleI    AlwNI     BanI     BbeI     BcgI    BsaBI
    BsaHI    BseYI  BsiHKAI Bsp1286I  BstZ17I   Bsu36I     BtgI    BtgZI
     EarI EcoO109I    EcoRV    HaeII     KasI     MmeI     MslI     NarI
     NcoI     NspI     PciI    PpuMI     SapI     SfcI     SfoI     StyI
    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI