Restriction map for
pFN21AE6006
(Linear) (Six-base) MAPSORT of: KIEE6006.seq Check: 920 from: 1 to: 942
KIEE6006.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 942
Size: 724 218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 922 942
Size: 264 658 20
Fragments arranged by size:
658 264 20
AleI CACnn'nnGTG
Cuts at: 0 22 736 942
Size: 22 714 206
Fragments arranged by size:
714 206 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 500 532 942
Size: 500 32 410
Fragments arranged by size:
500 410 32
AlwNI CAG_nnn'CTG
Cuts at: 0 287 805 942
Size: 287 518 137
Fragments arranged by size:
518 287 137
ApoI r'AATT_y
Cuts at: 0 31 942
Size: 31 911
AvaI C'yCGr_G
Cuts at: 0 59 942
Size: 59 883
BanII G_rGCy'C
Cuts at: 0 590 942
Size: 590 352
BbvCI CC'TCA_GC
Cuts at: 0 673 865 942
Size: 673 192 77
Fragments arranged by size:
673 192 77
BglII A'GATC_T
Cuts at: 0 249 942
Size: 249 693
Bme1580I G_kGCm'C
Cuts at: 0 758 942
Size: 758 184
BmrI ACTGGGnnnn_n'
Cuts at: 0 695 942
Size: 695 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 942
Size: 604 338
Bpu10I CC'TnA_GC
Cuts at: 0 673 865 942
Size: 673 192 77
Fragments arranged by size:
673 192 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 683 942
Size: 683 259
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 317 347 942
Size: 317 30 595
Fragments arranged by size:
595 317 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 631 942
Size: 631 311
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 549 702 942
Size: 373 176 153 240
Fragments arranged by size:
373 240 176 153
BsiHKAI G_wGCw'C
Cuts at: 0 590 942
Size: 590 352
BsmBI CGTCTCn'nnnn_
Cuts at: 0 900 942
Size: 900 42
Bsp1286I G_dGCh'C
Cuts at: 0 590 758 942
Size: 590 168 184
Fragments arranged by size:
590 184 168
BspHI T'CATG_A
Cuts at: 0 404 942
Size: 404 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 942
Size: 724 218
BsrGI T'GTAC_A
Cuts at: 0 101 942
Size: 101 841
BstAPI GCAn_nnn'nTGC
Cuts at: 0 753 942
Size: 753 189
BstEII G'GTnAC_C
Cuts at: 0 738 942
Size: 738 204
BstYI r'GATC_y
Cuts at: 0 249 942
Size: 249 693
Bsu36I CC'TnA_GG
Cuts at: 0 236 942
Size: 236 706
BtgI C'CryG_G
Cuts at: 0 734 942
Size: 734 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 432 551 942
Size: 432 119 391
Fragments arranged by size:
432 391 119
BtsI GCAGTG_nn'
Cuts at: 0 785 803 942
Size: 785 18 139
Fragments arranged by size:
785 139 18
EaeI y'GGCC_r
Cuts at: 0 887 942
Size: 887 55
EarI CTCTTCn'nnn_
Cuts at: 0 898 942
Size: 898 44
EcoICRI GAG'CTC
Cuts at: 0 588 942
Size: 588 354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 604 922 942
Size: 264 340 318 20
Fragments arranged by size:
340 318 264 20
EcoO109I rG'GnC_Cy
Cuts at: 0 240 549 942
Size: 240 309 393
Fragments arranged by size:
393 309 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 942
Size: 241 32 669
Fragments arranged by size:
669 241 32
MscI TGG'CCA
Cuts at: 0 889 942
Size: 889 53
MslI CAynn'nnrTG
Cuts at: 0 22 298 664 733 736 942
Size: 22 276 366 69 3 206
Fragments arranged by size:
366 276 206 69 22 3
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 942
Size: 318 624
PpuMI rG'GwC_Cy
Cuts at: 0 240 549 942
Size: 240 309 393
Fragments arranged by size:
393 309 240
SacI G_AGCT'C
Cuts at: 0 590 942
Size: 590 352
StuI AGG'CCT
Cuts at: 0 428 942
Size: 428 514
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 942
Size: 435 507
TatI w'GTAC_w
Cuts at: 0 101 176 942
Size: 101 75 766
Fragments arranged by size:
766 101 75
Enzymes that do cut:
AarI AcuI AleI AloI AlwNI ApoI AvaI BanII
BbvCI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaXI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrGI
BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI EcoICRI Eco57MI EcoO109I Hin4I MscI MslI PflMI
PpuMI SacI StuI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BspEI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstXI BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI