Restriction map for
pFN21AE6005
(Linear) (Six-base) MAPSORT of: KIEE6005.seq Check: 3849 from: 1 to: 942
KIEE6005.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 80 942
Size: 80 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 764 942
Size: 655 109 178
Fragments arranged by size:
655 178 109
AflIII A'CryG_T
Cuts at: 0 724 942
Size: 724 218
AleI CACnn'nnGTG
Cuts at: 0 766 942
Size: 766 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 942
Size: 409 533
AvaI C'yCGr_G
Cuts at: 0 455 942
Size: 455 487
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 166 199 942
Size: 166 33 743
Fragments arranged by size:
743 166 33
BanI G'GyrC_C
Cuts at: 0 742 942
Size: 742 200
BbvCI CC'TCA_GC
Cuts at: 0 231 942
Size: 231 711
BglI GCCn_nnn'nGGC
Cuts at: 0 202 298 942
Size: 202 96 644
Fragments arranged by size:
644 202 96
BlpI GC'TnA_GC
Cuts at: 0 102 942
Size: 102 840
BmrI ACTGGGnnnn_n'
Cuts at: 0 425 514 942
Size: 425 89 428
Fragments arranged by size:
428 425 89
Bpu10I CC'TnA_GC
Cuts at: 0 231 942
Size: 231 711
BsaBI GATnn'nnATC
Cuts at: 0 88 942
Size: 88 854
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 942
Size: 310 30 602
Fragments arranged by size:
602 310 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 942
Size: 219 723
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 117 379 942
Size: 58 59 262 563
Fragments arranged by size:
563 262 59 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 644 942
Size: 80 564 298
Fragments arranged by size:
564 298 80
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 942
Size: 216 726
BstYI r'GATC_y
Cuts at: 0 83 942
Size: 83 859
BtgI C'CryG_G
Cuts at: 0 356 860 942
Size: 356 504 82
Fragments arranged by size:
504 356 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 588 942
Size: 588 354
BtsI GCAGTG_nn'
Cuts at: 0 235 350 391 531 942
Size: 235 115 41 140 411
Fragments arranged by size:
411 235 140 115 41
EaeI y'GGCC_r
Cuts at: 0 194 353 611 942
Size: 194 159 258 331
Fragments arranged by size:
331 258 194 159
EarI CTCTTCn'nnn_
Cuts at: 0 783 856 942
Size: 783 73 86
Fragments arranged by size:
783 86 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 764 942
Size: 655 109 178
Fragments arranged by size:
655 178 109
EcoO109I rG'GnC_Cy
Cuts at: 0 408 419 942
Size: 408 11 523
Fragments arranged by size:
523 408 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 611 942
Size: 55 556 331
Fragments arranged by size:
556 331 55
MscI TGG'CCA
Cuts at: 0 196 355 613 942
Size: 196 159 258 329
Fragments arranged by size:
329 258 196 159
MslI CAynn'nnrTG
Cuts at: 0 553 607 740 766 942
Size: 553 54 133 26 176
Fragments arranged by size:
553 176 133 54 26
NcoI C'CATG_G
Cuts at: 0 356 860 942
Size: 356 504 82
Fragments arranged by size:
504 356 82
NspI r_CATG'y
Cuts at: 0 728 942
Size: 728 214
PciI A'CATG_T
Cuts at: 0 724 942
Size: 724 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 260 942
Size: 260 682
PpuMI rG'GwC_Cy
Cuts at: 0 408 419 942
Size: 408 11 523
Fragments arranged by size:
523 408 11
PshAI GACnn'nnGTC
Cuts at: 0 727 942
Size: 727 215
SanDI GG'GwC_CC
Cuts at: 0 419 942
Size: 419 523
SapI GCTCTTCn'nnn_
Cuts at: 0 856 942
Size: 856 86
SexAI A'CCwGG_T
Cuts at: 0 215 942
Size: 215 727
SmaI CCC'GGG
Cuts at: 0 457 942
Size: 457 485
SrfI GCCC'GGGC
Cuts at: 0 457 942
Size: 457 485
StuI AGG'CCT
Cuts at: 0 62 942
Size: 62 880
StyI C'CwwG_G
Cuts at: 0 356 404 860 942
Size: 356 48 456 82
Fragments arranged by size:
456 356 82 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 873 942
Size: 873 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 470 502 942
Size: 310 32 128 32 440
Fragments arranged by size:
440 310 128 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 217 417 942
Size: 217 200 525
Fragments arranged by size:
525 217 200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 621 942
Size: 438 183 321
Fragments arranged by size:
438 321 183
XmaI C'CCGG_G
Cuts at: 0 455 942
Size: 455 487
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AvaI BaeI BanI
BbvCI BglI BlpI BmrI Bpu10I BsaBI BsaXI BseRI
BsgI BspMI BstXI BstYI BtgI BtgZI BtsI EaeI
EarI Eco57MI EcoO109I MmeI MscI MslI NcoI NspI
PciI PflMI PpuMI PshAI SanDI SapI SexAI SmaI
SrfI StuI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII Bme1580I BmgBI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SspI SwaI TatI
XbaI XhoI XmnI ZraI