Restriction map for
pFN21AE6004
(Linear) (Six-base) MAPSORT of: KIEE6004.seq Check: 991 from: 1 to: 942
KIEE6004.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 942
Size: 727 215
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 574 942
Size: 36 538 368
Fragments arranged by size:
538 368 36
AflIII A'CryG_T
Cuts at: 0 659 942
Size: 659 283
AleI CACnn'nnGTG
Cuts at: 0 739 942
Size: 739 203
AlwNI CAG_nnn'CTG
Cuts at: 0 387 942
Size: 387 555
BanI G'GyrC_C
Cuts at: 0 268 540 757 780 942
Size: 268 272 217 23 162
Fragments arranged by size:
272 268 217 162 23
BanII G_rGCy'C
Cuts at: 0 439 749 942
Size: 439 310 193
Fragments arranged by size:
439 310 193
BbvCI CC'TCA_GC
Cuts at: 0 329 942
Size: 329 613
BciVI GTATCCnnnnn_n'
Cuts at: 0 404 942
Size: 404 538
BglI GCCn_nnn'nGGC
Cuts at: 0 119 942
Size: 119 823
BlpI GC'TnA_GC
Cuts at: 0 187 913 942
Size: 187 726 29
Fragments arranged by size:
726 187 29
Bpu10I CC'TnA_GC
Cuts at: 0 329 942
Size: 329 613
BsaAI yAC'GTr
Cuts at: 0 660 942
Size: 660 282
BsaBI GATnn'nnATC
Cuts at: 0 11 942
Size: 11 931
BsaHI Gr'CG_yC
Cuts at: 0 335 411 942
Size: 335 76 531
Fragments arranged by size:
531 335 76
BsaWI w'CCGG_w
Cuts at: 0 882 942
Size: 882 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 59 89 493 523 942
Size: 59 30 404 30 419
Fragments arranged by size:
419 404 59 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 429 706 714 942
Size: 67 362 277 8 228
Fragments arranged by size:
362 277 228 67 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 587 942
Size: 311 276 355
Fragments arranged by size:
355 311 276
BsiHKAI G_wGCw'C
Cuts at: 0 461 942
Size: 461 481
BsmBI CGTCTCn'nnnn_
Cuts at: 0 402 942
Size: 402 540
Bsp1286I G_dGCh'C
Cuts at: 0 439 461 749 942
Size: 439 22 288 193
Fragments arranged by size:
439 288 193 22
BspEI T'CCGG_A
Cuts at: 0 882 942
Size: 882 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 942
Size: 727 215
BsrGI T'GTAC_A
Cuts at: 0 773 942
Size: 773 169
BstEII G'GTnAC_C
Cuts at: 0 225 942
Size: 225 717
Bsu36I CC'TnA_GG
Cuts at: 0 603 942
Size: 603 339
BtgI C'CryG_G
Cuts at: 0 221 942
Size: 221 721
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 624 942
Size: 624 318
EaeI y'GGCC_r
Cuts at: 0 654 942
Size: 654 288
EarI CTCTTCn'nnn_
Cuts at: 0 55 85 142 832 942
Size: 55 30 57 690 110
Fragments arranged by size:
690 110 57 55 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 574 942
Size: 36 538 368
Fragments arranged by size:
538 368 36
HaeII r_GCGC'y
Cuts at: 0 595 942
Size: 595 347
MslI CAynn'nnrTG
Cuts at: 0 40 316 739 775 942
Size: 40 276 423 36 167
Fragments arranged by size:
423 276 167 40 36
NcoI C'CATG_G
Cuts at: 0 221 942
Size: 221 721
NspI r_CATG'y
Cuts at: 0 684 942
Size: 684 258
PmlI CAC'GTG
Cuts at: 0 660 942
Size: 660 282
PstI C_TGCA'G
Cuts at: 0 392 942
Size: 392 550
SfcI C'TryA_G
Cuts at: 0 388 831 867 942
Size: 388 443 36 75
Fragments arranged by size:
443 388 75 36
SphI G_CATG'C
Cuts at: 0 684 942
Size: 684 258
SspI AAT'ATT
Cuts at: 0 302 942
Size: 302 640
StuI AGG'CCT
Cuts at: 0 113 942
Size: 113 829
StyI C'CwwG_G
Cuts at: 0 221 942
Size: 221 721
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 310 435 942
Size: 186 124 125 507
Fragments arranged by size:
507 186 125 124
TatI w'GTAC_w
Cuts at: 0 176 399 773 942
Size: 176 223 374 169
Fragments arranged by size:
374 223 176 169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 942
Size: 59 32 851
Fragments arranged by size:
851 59 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 942
Size: 237 705
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI BanI BanII BbvCI
BciVI BglI BlpI Bpu10I BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrGI BstEII Bsu36I BtgI BtgZI EaeI EarI Eco57MI
HaeII MslI NcoI NspI PmlI PstI SfcI SphI
SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BcgI BclI
BfrBI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BseYI BsiEI BsiWI BsmI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI