Restriction map for
pFN21AE6003
(Linear) (Six-base) MAPSORT of: KIEE6003.seq Check: 2685 from: 1 to: 942
KIEE6003.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 942
Size: 574 368
AleI CACnn'nnGTG
Cuts at: 0 838 942
Size: 838 104
AvaI C'yCGr_G
Cuts at: 0 575 942
Size: 575 367
BanII G_rGCy'C
Cuts at: 0 412 614 900 942
Size: 412 202 286 42
Fragments arranged by size:
412 286 202 42
BglI GCCn_nnn'nGGC
Cuts at: 0 433 942
Size: 433 509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 942
Size: 349 593
Bpu10I CC'TnA_GC
Cuts at: 0 901 942
Size: 901 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 942
Size: 661 281
BsaI GGTCTCn'nnnn_
Cuts at: 0 317 775 942
Size: 317 458 167
Fragments arranged by size:
458 317 167
BsaBI GATnn'nnATC
Cuts at: 0 598 942
Size: 598 344
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 657 942
Size: 118 539 285
Fragments arranged by size:
539 285 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 527 942
Size: 373 154 415
Fragments arranged by size:
415 373 154
Bsp1286I G_dGCh'C
Cuts at: 0 412 614 900 942
Size: 412 202 286 42
Fragments arranged by size:
412 286 202 42
BspHI T'CATG_A
Cuts at: 0 404 942
Size: 404 538
BsrGI T'GTAC_A
Cuts at: 0 830 942
Size: 830 112
BstEII G'GTnAC_C
Cuts at: 0 840 942
Size: 840 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 942
Size: 117 825
Bsu36I CC'TnA_GG
Cuts at: 0 874 942
Size: 874 68
BtgI C'CryG_G
Cuts at: 0 350 942
Size: 350 592
EaeI y'GGCC_r
Cuts at: 0 95 347 371 488 942
Size: 95 252 24 117 454
Fragments arranged by size:
454 252 117 95 24
EarI CTCTTCn'nnn_
Cuts at: 0 225 942
Size: 225 717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 574 942
Size: 349 225 368
Fragments arranged by size:
368 349 225
EcoO109I rG'GnC_Cy
Cuts at: 0 264 647 681 942
Size: 264 383 34 261
Fragments arranged by size:
383 264 261 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 579 942
Size: 579 363
HaeII r_GCGC'y
Cuts at: 0 439 942
Size: 439 503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 942
Size: 81 861
MscI TGG'CCA
Cuts at: 0 97 349 373 490 942
Size: 97 252 24 117 452
Fragments arranged by size:
452 252 117 97 24
MslI CAynn'nnrTG
Cuts at: 0 172 817 823 838 942
Size: 172 645 6 15 104
Fragments arranged by size:
645 172 104 15 6
NcoI C'CATG_G
Cuts at: 0 350 942
Size: 350 592
NspI r_CATG'y
Cuts at: 0 255 942
Size: 255 687
PpuMI rG'GwC_Cy
Cuts at: 0 264 647 681 942
Size: 264 383 34 261
Fragments arranged by size:
383 264 261 34
PspXI vC'TCGA_Gb
Cuts at: 0 575 942
Size: 575 367
SfcI C'TryA_G
Cuts at: 0 867 942
Size: 867 75
SmlI C'TyrA_G
Cuts at: 0 575 676 942
Size: 575 101 266
Fragments arranged by size:
575 266 101
StuI AGG'CCT
Cuts at: 0 709 942
Size: 709 233
StyI C'CwwG_G
Cuts at: 0 350 642 942
Size: 350 292 300
Fragments arranged by size:
350 300 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 579 942
Size: 435 144 363
Fragments arranged by size:
435 363 144
TatI w'GTAC_w
Cuts at: 0 302 830 942
Size: 302 528 112
Fragments arranged by size:
528 302 112
Tth111I GACn'n_nGTC
Cuts at: 0 814 942
Size: 814 128
XhoI C'TCGA_G
Cuts at: 0 575 942
Size: 575 367
XmnI GAAnn'nnTTC
Cuts at: 0 859 928 942
Size: 859 69 14
Fragments arranged by size:
859 69 14
Enzymes that do cut:
AcuI AleI AvaI BanII BglI BpmI Bpu10I BpuEI
BsaI BsaBI BseRI BsgI Bsp1286I BspHI BsrGI BstEII
BstXI Bsu36I BtgI EaeI EarI Eco57MI EcoO109I FalI
HaeII MmeI MscI MslI NcoI NspI PpuMI PspXI
SfcI SmlI StuI StyI TaqII TatI Tth111I XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BsaAI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XcmI XmaI ZraI