(Linear) (Six-base) MAPSORT of: KIEE5996.seq  Check: 8338  from: 1  to: 942



KIEE5996.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:18  ..

AccI GT'mk_AC

Cuts at:      0     692     942 
   Size:        692     250
 
AclI AA'CG_TT

Cuts at:      0      82     942 
   Size:         82     860
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     671     942 
   Size:        200     471     271

  Fragments arranged by size: 

                 471     271     200
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     387     942 
   Size:        387     555
 
BanI G'GyrC_C

Cuts at:      0     813     942 
   Size:        813     129
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     263     942 
   Size:        263     679
 
BfrBI ATG'CAT

Cuts at:      0     280     942 
   Size:        280     662
 
BglI GCCn_nnn'nGGC

Cuts at:      0     346     942 
   Size:        346     596
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     482     942 
   Size:        482     460
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     768     942 
   Size:        768     174
 
BseYI C'CCAG_C

Cuts at:      0     778     942 
   Size:        778     164
 
BsiHKAI G_wGCw'C

Cuts at:      0     420     942 
   Size:        420     522
 
BsmI GAATG_Cn'

Cuts at:      0     462     892     942 
   Size:        462     430      50

  Fragments arranged by size: 

                 462     430      50
 
Bsp1286I G_dGCh'C

Cuts at:      0     420     942 
   Size:        420     522
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      54     942 
   Size:         54     888
 
BsrDI GCAATG_nn'

Cuts at:      0     728     942 
   Size:        728     214
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     286     942 
   Size:        286     656
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237     942 
   Size:        237     705
 
BstZ17I GTA'TAC

Cuts at:      0     693     942 
   Size:        693     249
 
BtgI C'CryG_G

Cuts at:      0     794     942 
   Size:        794     148
 
BtsI GCAGTG_nn'

Cuts at:      0     374     942 
   Size:        374     568
 
EaeI y'GGCC_r

Cuts at:      0     425     942 
   Size:        425     517
 
EarI CTCTTCn'nnn_

Cuts at:      0     219     880     942 
   Size:        219     661      62

  Fragments arranged by size: 

                 661     219      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     671     768     942 
   Size:        200     471      97     174

  Fragments arranged by size: 

                 471     200     174      97
 
EcoRV GAT'ATC

Cuts at:      0     641     942 
   Size:        641     301
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     942 
   Size:         93     849
 
MscI TGG'CCA

Cuts at:      0     427     942 
   Size:        427     515
 
MslI CAynn'nnrTG

Cuts at:      0     235     397     841     942 
   Size:        235     162     444     101

  Fragments arranged by size: 

                 444     235     162     101
 
MspA1I CmG'CkG

Cuts at:      0     220     942 
   Size:        220     722
 
NcoI C'CATG_G

Cuts at:      0     794     942 
   Size:        794     148
 
NsiI A_TGCA'T

Cuts at:      0     282     942 
   Size:        282     660
 
NspI r_CATG'y

Cuts at:      0     284     942 
   Size:        284     658
 
PsiI TTA'TAA

Cuts at:      0      79     942 
   Size:         79     863
 
PstI C_TGCA'G

Cuts at:      0      65     942 
   Size:         65     877
 
PvuII CAG'CTG

Cuts at:      0     220     942 
   Size:        220     722
 
SfcI C'TryA_G

Cuts at:      0      61     861     926     942 
   Size:         61     800      65      16

  Fragments arranged by size: 

                 800      65      61      16
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     346     942 
   Size:        346     596
 
StyI C'CwwG_G

Cuts at:      0     794     942 
   Size:        794     148
 
TatI w'GTAC_w

Cuts at:      0     170     767     942 
   Size:        170     597     175

  Fragments arranged by size: 

                 597     175     170
 
Tth111I GACn'n_nGTC

Cuts at:      0     262     942 
   Size:        262     680
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     792     942 
   Size:        792     150
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AlfI     BanI     BbsI    BfrBI     BglI
     BmrI     BpmI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI
   BstAPI    BstXI  BstZ17I     BtgI     BtsI     EaeI     EarI  Eco57MI
    EcoRV     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
     PsiI     PstI    PvuII     SfcI     SfiI     StyI     TatI  Tth111I
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI
     XmaI     XmnI     ZraI