Restriction map for
pFN21AE5994
(Linear) (Six-base) MAPSORT of: KIEE5994.seq Check: 6844 from: 1 to: 942
KIEE5994.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 674 885 942
Size: 574 100 211 57
Fragments arranged by size:
574 211 100 57
AflII C'TTAA_G
Cuts at: 0 873 942
Size: 873 69
AflIII A'CryG_T
Cuts at: 0 718 942
Size: 718 224
AleI CACnn'nnGTG
Cuts at: 0 739 942
Size: 739 203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 942
Size: 403 539
AlwNI CAG_nnn'CTG
Cuts at: 0 419 942
Size: 419 523
ApoI r'AATT_y
Cuts at: 0 31 942
Size: 31 911
BanII G_rGCy'C
Cuts at: 0 762 942
Size: 762 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 680 815 819 942
Size: 680 135 4 123
Fragments arranged by size:
680 135 123 4
BglI GCCn_nnn'nGGC
Cuts at: 0 253 942
Size: 253 689
BmrI ACTGGGnnnn_n'
Cuts at: 0 291 942
Size: 291 651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 823 907 942
Size: 444 379 84 35
Fragments arranged by size:
444 379 84 35
Bpu10I CC'TnA_GC
Cuts at: 0 187 789 934 942
Size: 187 602 145 8
Fragments arranged by size:
602 187 145 8
BsaBI GATnn'nnATC
Cuts at: 0 592 942
Size: 592 350
BsaHI Gr'CG_yC
Cuts at: 0 896 942
Size: 896 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 431 461 942
Size: 431 30 481
Fragments arranged by size:
481 431 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 942
Size: 341 601
Bsp1286I G_dGCh'C
Cuts at: 0 762 942
Size: 762 180
BstAPI GCAn_nnn'nTGC
Cuts at: 0 332 942
Size: 332 610
BstYI r'GATC_y
Cuts at: 0 239 942
Size: 239 703
BtgI C'CryG_G
Cuts at: 0 398 942
Size: 398 544
BtsI GCAGTG_nn'
Cuts at: 0 353 385 573 734 942
Size: 353 32 188 161 208
Fragments arranged by size:
353 208 188 161 32
DraIII CAC_nnn'GTG
Cuts at: 0 332 942
Size: 332 610
EaeI y'GGCC_r
Cuts at: 0 2 254 282 401 623 942
Size: 2 252 28 119 222 319
Fragments arranged by size:
319 252 222 119 28 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 574 674 823 885 907 942
Size: 444 130 100 149 62 22 35
Fragments arranged by size:
444 149 130 100 62 35 22
EcoRI G'AATT_C
Cuts at: 0 31 942
Size: 31 911
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 431 463 636 668 749 781 942
Size: 431 32 173 32 81 32 161
Fragments arranged by size:
431 173 161 81 32 32 32
HindIII A'AGCT_T
Cuts at: 0 556 942
Size: 556 386
MscI TGG'CCA
Cuts at: 0 4 256 403 625 942
Size: 4 252 147 222 317
Fragments arranged by size:
317 252 222 147 4
MslI CAynn'nnrTG
Cuts at: 0 40 172 739 847 942
Size: 40 132 567 108 95
Fragments arranged by size:
567 132 108 95 40
NspI r_CATG'y
Cuts at: 0 722 942
Size: 722 220
PciI A'CATG_T
Cuts at: 0 718 942
Size: 718 224
PstI C_TGCA'G
Cuts at: 0 568 942
Size: 568 374
SfcI C'TryA_G
Cuts at: 0 564 867 942
Size: 564 303 75
Fragments arranged by size:
564 303 75
SmlI C'TyrA_G
Cuts at: 0 873 942
Size: 873 69
TatI w'GTAC_w
Cuts at: 0 176 942
Size: 176 766
Tth111I GACn'n_nGTC
Cuts at: 0 814 942
Size: 814 128
XmnI GAAnn'nnTTC
Cuts at: 0 35 942
Size: 35 907
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI AlwNI ApoI BanII
BbsI BglI BmrI BpmI Bpu10I BsaBI BsaHI BsaXI
BsgI Bsp1286I BstAPI BstYI BtgI BtsI DraIII EaeI
Eco57MI EcoRI Hin4I HindIII MscI MslI NspI PciI
PstI SfcI SmlI TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI XbaI XcmI XhoI XmaI ZraI