Restriction map for
pFN21AE5993
(Linear) (Six-base) MAPSORT of: KIEE5993.seq Check: 1672 from: 1 to: 942
KIEE5993.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AccI GT'mk_AC
Cuts at: 0 860 942
Size: 860 82
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 942
Size: 707 235
AleI CACnn'nnGTG
Cuts at: 0 16 942
Size: 16 926
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 942
Size: 50 32 860
Fragments arranged by size:
860 50 32
ApoI r'AATT_y
Cuts at: 0 25 262 942
Size: 25 237 680
Fragments arranged by size:
680 237 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 75 942
Size: 75 867
BbvCI CC'TCA_GC
Cuts at: 0 216 942
Size: 216 726
BfrBI ATG'CAT
Cuts at: 0 460 942
Size: 460 482
Bme1580I G_kGCm'C
Cuts at: 0 802 942
Size: 802 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 482 942
Size: 482 460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 942
Size: 768 174
Bpu10I CC'TnA_GC
Cuts at: 0 216 942
Size: 216 726
BsaHI Gr'CG_yC
Cuts at: 0 693 942
Size: 693 249
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 527 530 942
Size: 527 3 412
Fragments arranged by size:
527 412 3
BsiHKAI G_wGCw'C
Cuts at: 0 420 942
Size: 420 522
BsmI GAATG_Cn'
Cuts at: 0 462 942
Size: 462 480
Bsp1286I G_dGCh'C
Cuts at: 0 420 802 942
Size: 420 382 140
Fragments arranged by size:
420 382 140
BspHI T'CATG_A
Cuts at: 0 668 942
Size: 668 274
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 942
Size: 237 705
BstYI r'GATC_y
Cuts at: 0 65 502 942
Size: 65 437 440
Fragments arranged by size:
440 437 65
Bsu36I CC'TnA_GG
Cuts at: 0 868 942
Size: 868 74
BtsI GCAGTG_nn'
Cuts at: 0 317 942
Size: 317 625
DraI TTT'AAA
Cuts at: 0 897 942
Size: 897 45
EaeI y'GGCC_r
Cuts at: 0 425 942
Size: 425 517
EarI CTCTTCn'nnn_
Cuts at: 0 79 942
Size: 79 863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 768 942
Size: 707 61 174
Fragments arranged by size:
707 174 61
EcoRI G'AATT_C
Cuts at: 0 25 942
Size: 25 917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 909 942
Size: 909 33
HincII GTy'rAC
Cuts at: 0 44 942
Size: 44 898
HpaI GTT'AAC
Cuts at: 0 44 942
Size: 44 898
MscI TGG'CCA
Cuts at: 0 427 942
Size: 427 515
MslI CAynn'nnrTG
Cuts at: 0 16 34 166 397 667 673 841 942
Size: 16 18 132 231 270 6 168 101
Fragments arranged by size:
270 231 168 132 101 18 16 6
MspA1I CmG'CkG
Cuts at: 0 220 942
Size: 220 722
NsiI A_TGCA'T
Cuts at: 0 462 942
Size: 462 480
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 942
Size: 795 147
PshAI GACnn'nnGTC
Cuts at: 0 152 942
Size: 152 790
PstI C_TGCA'G
Cuts at: 0 65 324 735 942
Size: 65 259 411 207
Fragments arranged by size:
411 259 207 65
PvuII CAG'CTG
Cuts at: 0 220 942
Size: 220 722
SfcI C'TryA_G
Cuts at: 0 61 320 498 731 861 905 926 942
Size: 61 259 178 233 130 44 21 16
Fragments arranged by size:
259 233 178 130 61 44 21 16
StuI AGG'CCT
Cuts at: 0 703 922 942
Size: 703 219 20
Fragments arranged by size:
703 219 20
StyI C'CwwG_G
Cuts at: 0 230 942
Size: 230 712
TatI w'GTAC_w
Cuts at: 0 170 767 942
Size: 170 597 175
Fragments arranged by size:
597 175 170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 792 942
Size: 237 555 150
Fragments arranged by size:
555 237 150
XmnI GAAnn'nnTTC
Cuts at: 0 137 481 942
Size: 137 344 461
Fragments arranged by size:
461 344 137
Enzymes that do cut:
AccI AcuI AleI AloI ApoI BbsI BbvCI BfrBI
Bme1580I BmrI BpmI Bpu10I BsaHI BseRI BsiHKAI BsmI
Bsp1286I BspHI BstXI BstYI Bsu36I BtsI DraI EaeI
EarI Eco57MI EcoRI FalI HincII HpaI MscI MslI
MspA1I NsiI PflMI PshAI PstI PvuII SfcI StuI
StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII Hin4I
HindIII KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI