(Linear) (Six-base) MAPSORT of: KIEE5990.seq  Check: 5074  from: 1  to: 942



KIEE5990.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:18  ..

AccI GT'mk_AC

Cuts at:      0      19     521     942 
   Size:         19     502     421

  Fragments arranged by size: 

                 502     421      19
 
AflIII A'CryG_T

Cuts at:      0     773     942 
   Size:        773     169
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403     942 
   Size:        403     539
 
ApoI r'AATT_y

Cuts at:      0      31     918     942 
   Size:         31     887      24

  Fragments arranged by size: 

                 887      31      24
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     563     596     942 
   Size:        563      33     346

  Fragments arranged by size: 

                 563     346      33
 
BanII G_rGCy'C

Cuts at:      0      50     942 
   Size:         50     892
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     732     942 
   Size:        732     210
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     564     942 
   Size:        564     378
 
BfrBI ATG'CAT

Cuts at:      0     462     761     942 
   Size:        462     299     181

  Fragments arranged by size: 

                 462     299     181
 
BseYI C'CCAG_C

Cuts at:      0      42     942 
   Size:         42     900
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     942 
   Size:        373     569
 
BsmI GAATG_Cn'

Cuts at:      0     462     942 
   Size:        462     480
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     942 
   Size:         50     892
 
BsrDI GCAATG_nn'

Cuts at:      0      94     938     942 
   Size:         94     844       4

  Fragments arranged by size: 

                 844      94       4
 
BsrGI T'GTAC_A

Cuts at:      0     718     942 
   Size:        718     224
 
BssSI C'ACGA_G

Cuts at:      0     430     942 
   Size:        430     512
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      98     133     942 
   Size:         98      35     809

  Fragments arranged by size: 

                 809      98      35
 
EaeI y'GGCC_r

Cuts at:      0     347     778     942 
   Size:        347     431     164

  Fragments arranged by size: 

                 431     347     164
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     360     942 
   Size:        360     582
 
EcoO109I rG'GnC_Cy

Cuts at:      0     705     942 
   Size:        705     237
 
MscI TGG'CCA

Cuts at:      0     349     780     942 
   Size:        349     431     162

  Fragments arranged by size: 

                 431     349     162
 
MslI CAynn'nnrTG

Cuts at:      0     208     349     772     942 
   Size:        208     141     423     170

  Fragments arranged by size: 

                 423     208     170     141
 
NdeI CA'TA_TG

Cuts at:      0     651     942 
   Size:        651     291
 
NsiI A_TGCA'T

Cuts at:      0     464     763     942 
   Size:        464     299     179

  Fragments arranged by size: 

                 464     299     179
 
NspI r_CATG'y

Cuts at:      0     777     942 
   Size:        777     165
 
PciI A'CATG_T

Cuts at:      0     773     942 
   Size:        773     169
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     505     942 
   Size:        505     437
 
PfoI T'CCnGG_A

Cuts at:      0     442     942 
   Size:        442     500
 
SfcI C'TryA_G

Cuts at:      0      67     942 
   Size:         67     875
 
TatI w'GTAC_w

Cuts at:      0     718     942 
   Size:        718     224
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     319     942 
   Size:        319     623
 

 Enzymes that do cut:

     AccI   AflIII     AlfI     ApoI     BaeI    BanII     BbsI    BciVI
    BfrBI    BseYI     BsgI     BsmI Bsp1286I    BsrDI    BsrGI    BssSI
    CspCI     EaeI    EcoNI EcoO109I     MscI     MslI     NdeI     NsiI
     NspI     PciI    PflMI     PfoI     SfcI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI