Restriction map for
pFN21AE5986
(Linear) (Six-base) MAPSORT of: KIEE5986.seq Check: 9021 from: 1 to: 939
KIEE5986.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
Acc65I G'GTAC_C
Cuts at: 0 624 939
Size: 624 315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 939
Size: 696 243
AflIII A'CryG_T
Cuts at: 0 338 939
Size: 338 601
AleI CACnn'nnGTG
Cuts at: 0 835 939
Size: 835 104
ApoI r'AATT_y
Cuts at: 0 31 153 917 939
Size: 31 122 764 22
Fragments arranged by size:
764 122 31 22
BanI G'GyrC_C
Cuts at: 0 624 840 939
Size: 624 216 99
Fragments arranged by size:
624 216 99
BclI T'GATC_A
Cuts at: 0 134 939
Size: 134 805
Bme1580I G_kGCm'C
Cuts at: 0 845 939
Size: 845 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 623 939
Size: 623 316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 923 939
Size: 923 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 538 939
Size: 482 56 401
Fragments arranged by size:
482 401 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 939
Size: 677 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 533 563 939
Size: 533 30 376
Fragments arranged by size:
533 376 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 541 939
Size: 210 331 398
Fragments arranged by size:
398 331 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 939
Size: 549 390
BsmI GAATG_Cn'
Cuts at: 0 671 766 939
Size: 671 95 173
Fragments arranged by size:
671 173 95
Bsp1286I G_dGCh'C
Cuts at: 0 845 939
Size: 845 94
BsrDI GCAATG_nn'
Cuts at: 0 385 734 939
Size: 385 349 205
Fragments arranged by size:
385 349 205
BstEII G'GTnAC_C
Cuts at: 0 399 939
Size: 399 540
BstYI r'GATC_y
Cuts at: 0 793 939
Size: 793 146
BtgI C'CryG_G
Cuts at: 0 113 467 939
Size: 113 354 472
Fragments arranged by size:
472 354 113
BtsI GCAGTG_nn'
Cuts at: 0 513 939
Size: 513 426
DraIII CAC_nnn'GTG
Cuts at: 0 482 939
Size: 482 457
EaeI y'GGCC_r
Cuts at: 0 371 939
Size: 371 568
EarI CTCTTCn'nnn_
Cuts at: 0 193 939
Size: 193 746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 538 696 939
Size: 482 56 158 243
Fragments arranged by size:
482 243 158 56
HincII GTy'rAC
Cuts at: 0 344 582 939
Size: 344 238 357
Fragments arranged by size:
357 344 238
KpnI G_GTAC'C
Cuts at: 0 628 939
Size: 628 311
MscI TGG'CCA
Cuts at: 0 373 939
Size: 373 566
MslI CAynn'nnrTG
Cuts at: 0 172 604 835 939
Size: 172 432 231 104
Fragments arranged by size:
432 231 172 104
MspA1I CmG'CkG
Cuts at: 0 763 939
Size: 763 176
NcoI C'CATG_G
Cuts at: 0 113 939
Size: 113 826
NdeI CA'TA_TG
Cuts at: 0 357 939
Size: 357 582
NspI r_CATG'y
Cuts at: 0 342 489 939
Size: 342 147 450
Fragments arranged by size:
450 342 147
PciI A'CATG_T
Cuts at: 0 338 939
Size: 338 601
PstI C_TGCA'G
Cuts at: 0 508 939
Size: 508 431
ScaI AGT'ACT
Cuts at: 0 717 939
Size: 717 222
SfcI C'TryA_G
Cuts at: 0 504 864 939
Size: 504 360 75
Fragments arranged by size:
504 360 75
SmlI C'TyrA_G
Cuts at: 0 692 939
Size: 692 247
SphI G_CATG'C
Cuts at: 0 489 939
Size: 489 450
SspI AAT'ATT
Cuts at: 0 270 784 939
Size: 270 514 155
Fragments arranged by size:
514 270 155
StyI C'CwwG_G
Cuts at: 0 113 126 939
Size: 113 13 813
Fragments arranged by size:
813 113 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 275 939
Size: 136 139 664
Fragments arranged by size:
664 139 136
TatI w'GTAC_w
Cuts at: 0 176 715 939
Size: 176 539 224
Fragments arranged by size:
539 224 176
XmnI GAAnn'nnTTC
Cuts at: 0 35 813 939
Size: 35 778 126
Fragments arranged by size:
778 126 35
Enzymes that do cut:
Acc65I AcuI AflIII AleI ApoI BanI BclI Bme1580I
BmrI BplI BpmI BpuEI BsaXI BseRI BsgI BsmI
Bsp1286I BsrDI BstEII BstYI BtgI BtsI DraIII EaeI
EarI Eco57MI HincII KpnI MscI MslI MspA1I NcoI
NdeI NspI PciI PstI ScaI SfcI SmlI SphI
SspI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI MfeI MluI MmeI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI