(Linear) (Six-base) MAPSORT of: KIEE5980.seq  Check: 5121  from: 1  to: 939



KIEE5980.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     730     939 
   Size:        730     209
 
AatII G_ACGT'C

Cuts at:      0     417     939 
   Size:        417     522
 
AleI CACnn'nnGTG

Cuts at:      0      22     939 
   Size:         22     917
 
ApaI G_GGCC'C

Cuts at:      0     512     939 
   Size:        512     427
 
ApoI r'AATT_y

Cuts at:      0      31     939 
   Size:         31     908
 
AvaI C'yCGr_G

Cuts at:      0      59     939 
   Size:         59     880
 
BanII G_rGCy'C

Cuts at:      0     512     939 
   Size:        512     427
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     401     435     939 
   Size:        401      34     504

  Fragments arranged by size: 

                 504     401      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     436     939 
   Size:        436     503
 
BfrBI ATG'CAT

Cuts at:      0     290     939 
   Size:        290     649
 
Bme1580I G_kGCm'C

Cuts at:      0     512     939 
   Size:        512     427
 
Bpu10I CC'TnA_GC

Cuts at:      0     690     939 
   Size:        690     249
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     658     939 
   Size:        658     281
 
BsaHI Gr'CG_yC

Cuts at:      0     414     939 
   Size:        414     525
 
BsaWI w'CCGG_w

Cuts at:      0     554     939 
   Size:        554     385
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     598     939 
   Size:        598     341
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     421     939 
   Size:        421     518
 
Bsp1286I G_dGCh'C

Cuts at:      0     512     939 
   Size:        512     427
 
BspEI T'CCGG_A

Cuts at:      0     554     939 
   Size:        554     385
 
BspHI T'CATG_A

Cuts at:      0     404     939 
   Size:        404     535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     730     939 
   Size:        730     209
 
BsrDI GCAATG_nn'

Cuts at:      0     290     632     939 
   Size:        290     342     307

  Fragments arranged by size: 

                 342     307     290
 
BstEII G'GTnAC_C

Cuts at:      0     909     939 
   Size:        909      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     225     307     926     939 
   Size:        225      82     619      13

  Fragments arranged by size: 

                 619     225      82      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     939 
   Size:         93     846
 
MslI CAynn'nnrTG

Cuts at:      0      22     349     745     939 
   Size:         22     327     396     194

  Fragments arranged by size: 

                 396     327     194      22
 
NsiI A_TGCA'T

Cuts at:      0     292     939 
   Size:        292     647
 
PfoI T'CCnGG_A

Cuts at:      0     442     939 
   Size:        442     497
 
PspOMI G'GGCC_C

Cuts at:      0     508     939 
   Size:        508     431
 
SapI GCTCTTCn'nnn_

Cuts at:      0     307     939 
   Size:        307     632
 
SfcI C'TryA_G

Cuts at:      0     600     939 
   Size:        600     339
 
SmlI C'TyrA_G

Cuts at:      0     673     939 
   Size:        673     266
 
SpeI A'CTAG_T

Cuts at:      0      72     939 
   Size:         72     867
 
SspI AAT'ATT

Cuts at:      0     839     939 
   Size:        839     100
 
StyI C'CwwG_G

Cuts at:      0      20     939 
   Size:         20     919
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     536     549     568     581     939 
   Size:        536      13      19      13     358

  Fragments arranged by size: 

                 536     358      19      13      13
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     903     939 
   Size:        903      36
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     939 
   Size:         35     904
 
ZraI GAC'GTC

Cuts at:      0     415     939 
   Size:        415     524
 

 Enzymes that do cut:

     AarI    AatII     AleI     ApaI     ApoI     AvaI    BanII     BcgI
    BciVI    BfrBI Bme1580I   Bpu10I    BpuEI    BsaHI    BsaWI    BseRI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI   BstEII     EarI
     MmeI     MslI     NsiI     PfoI   PspOMI     SapI     SfcI     SmlI
     SpeI     SspI     StyI     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BclI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
     BsaI    BsaAI    BsaBI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI