(Linear) (Six-base) MAPSORT of: KIEE5978.seq  Check: 8553  from: 1  to: 936



KIEE5978.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:17  ..

AflIII A'CryG_T

Cuts at:      0      98     936 
   Size:         98     838
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     936 
   Size:        397     539
 
AlwNI CAG_nnn'CTG

Cuts at:      0      69     936 
   Size:         69     867
 
ApoI r'AATT_y

Cuts at:      0     186     325     493     673     849     936 
   Size:        186     139     168     180     176      87

  Fragments arranged by size: 

                 186     180     176     168     139      87
 
AseI AT'TA_AT

Cuts at:      0     574     803     936 
   Size:        574     229     133

  Fragments arranged by size: 

                 574     229     133
 
BamHI G'GATC_C

Cuts at:      0     147     936 
   Size:        147     789
 
BbvCI CC'TCA_GC

Cuts at:      0      56     936 
   Size:         56     880
 
BfrBI ATG'CAT

Cuts at:      0     280     535     936 
   Size:        280     255     401

  Fragments arranged by size: 

                 401     280     255
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     263     936 
   Size:        263     673
 
Bpu10I CC'TnA_GC

Cuts at:      0      56     477     936 
   Size:         56     421     459

  Fragments arranged by size: 

                 459     421      56
 
BspHI T'CATG_A

Cuts at:      0     398     897     936 
   Size:        398     499      39

  Fragments arranged by size: 

                 499     398      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      54     936 
   Size:         54     882
 
BsrDI GCAATG_nn'

Cuts at:      0     535     936 
   Size:        535     401
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      69     936 
   Size:         69     867
 
BstYI r'GATC_y

Cuts at:      0      51     147     936 
   Size:         51      96     789

  Fragments arranged by size: 

                 789      96      51
 
DraI TTT'AAA

Cuts at:      0     929     936 
   Size:        929       7
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     508     936 
   Size:        508     428
 
EaeI y'GGCC_r

Cuts at:      0     365     936 
   Size:        365     571
 
EarI CTCTTCn'nnn_

Cuts at:      0     785     936 
   Size:        785     151
 
EcoRI G'AATT_C

Cuts at:      0     493     849     936 
   Size:        493     356      87

  Fragments arranged by size: 

                 493     356      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      36      68     936 
   Size:         36      32     868

  Fragments arranged by size: 

                 868      36      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     418     936 
   Size:        125     293     518

  Fragments arranged by size: 

                 518     293     125
 
MscI TGG'CCA

Cuts at:      0     367     936 
   Size:        367     569
 
MslI CAynn'nnrTG

Cuts at:      0      97     936 
   Size:         97     839
 
MspA1I CmG'CkG

Cuts at:      0      60     755     936 
   Size:         60     695     181

  Fragments arranged by size: 

                 695     181      60
 
NdeI CA'TA_TG

Cuts at:      0     351     936 
   Size:        351     585
 
NsiI A_TGCA'T

Cuts at:      0     282     537     936 
   Size:        282     255     399

  Fragments arranged by size: 

                 399     282     255
 
NspI r_CATG'y

Cuts at:      0     102     284     936 
   Size:        102     182     652

  Fragments arranged by size: 

                 652     182     102
 
PciI A'CATG_T

Cuts at:      0      98     936 
   Size:         98     838
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     620     652     936 
   Size:        620      32     284

  Fragments arranged by size: 

                 620     284      32
 
PstI C_TGCA'G

Cuts at:      0      65     936 
   Size:         65     871
 
PvuII CAG'CTG

Cuts at:      0      60     755     936 
   Size:         60     695     181

  Fragments arranged by size: 

                 695     181      60
 
SfcI C'TryA_G

Cuts at:      0      61     936 
   Size:         61     875
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     180     936 
   Size:        180     756
 
XbaI T'CTAG_A

Cuts at:      0     489     936 
   Size:        489     447
 
XmnI GAAnn'nnTTC

Cuts at:      0     329     853     936 
   Size:        329     524      83

  Fragments arranged by size: 

                 524     329      83
 

 Enzymes that do cut:

   AflIII     AlfI    AlwNI     ApoI     AseI    BamHI    BbvCI    BfrBI
     BmrI   Bpu10I    BspHI    BspMI    BsrDI   BstAPI    BstYI     DraI
     DrdI     EaeI     EarI    EcoRI    Hin4I     MmeI     MscI     MslI
   MspA1I     NdeI     NsiI     NspI     PciI     PsrI     PstI    PvuII
     SfcI    TaqII     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI