Restriction map for
pFN21AE5975
(Linear) (Six-base) MAPSORT of: KIEE5975.seq Check: 9308 from: 1 to: 936
KIEE5975.orf
With 182 enzymes: SgfI *
December 5, 2014 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 936
Size: 724 212
AccI GT'mk_AC
Cuts at: 0 420 936
Size: 420 516
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 639 672 936
Size: 639 33 264
Fragments arranged by size:
639 264 33
BamHI G'GATC_C
Cuts at: 0 677 936
Size: 677 259
BanI G'GyrC_C
Cuts at: 0 263 319 754 921 936
Size: 263 56 435 167 15
Fragments arranged by size:
435 263 167 56 15
BanII G_rGCy'C
Cuts at: 0 646 936
Size: 646 290
BbeI G_GCGC'C
Cuts at: 0 267 323 936
Size: 267 56 613
Fragments arranged by size:
613 267 56
BciVI GTATCCnnnnn_n'
Cuts at: 0 667 936
Size: 667 269
BclI T'GATC_A
Cuts at: 0 731 936
Size: 731 205
BglI GCCn_nnn'nGGC
Cuts at: 0 629 936
Size: 629 307
BlpI GC'TnA_GC
Cuts at: 0 403 936
Size: 403 533
BmtI G_CTAG'C
Cuts at: 0 430 936
Size: 430 506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 145 277 936
Size: 49 96 132 659
Fragments arranged by size:
659 132 96 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 147 744 936
Size: 147 597 192
Fragments arranged by size:
597 192 147
BsaHI Gr'CG_yC
Cuts at: 0 264 320 936
Size: 264 56 616
Fragments arranged by size:
616 264 56
BsaWI w'CCGG_w
Cuts at: 0 674 936
Size: 674 262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 711 936
Size: 601 110 225
Fragments arranged by size:
601 225 110
BseYI C'CCAG_C
Cuts at: 0 430 465 936
Size: 430 35 471
Fragments arranged by size:
471 430 35
BsiHKAI G_wGCw'C
Cuts at: 0 291 936
Size: 291 645
Bsp1286I G_dGCh'C
Cuts at: 0 291 646 936
Size: 291 355 290
Fragments arranged by size:
355 291 290
BspEI T'CCGG_A
Cuts at: 0 674 936
Size: 674 262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 724 936
Size: 110 614 212
Fragments arranged by size:
614 212 110
BsrFI r'CCGG_y
Cuts at: 0 260 936
Size: 260 676
BstEII G'GTnAC_C
Cuts at: 0 837 936
Size: 837 99
BstYI r'GATC_y
Cuts at: 0 536 677 936
Size: 536 141 259
Fragments arranged by size:
536 259 141
Bsu36I CC'TnA_GG
Cuts at: 0 782 936
Size: 782 154
BtgI C'CryG_G
Cuts at: 0 17 110 323 347 936
Size: 17 93 213 24 589
Fragments arranged by size:
589 213 93 24 17
BtsI GCAGTG_nn'
Cuts at: 0 394 936
Size: 394 542
DraIII CAC_nnn'GTG
Cuts at: 0 329 414 936
Size: 329 85 522
Fragments arranged by size:
522 329 85
EaeI y'GGCC_r
Cuts at: 0 56 258 800 936
Size: 56 202 542 136
Fragments arranged by size:
542 202 136 56
EarI CTCTTCn'nnn_
Cuts at: 0 82 316 751 829 936
Size: 82 234 435 78 107
Fragments arranged by size:
435 234 107 82 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 145 277 936
Size: 49 96 132 659
Fragments arranged by size:
659 132 96 49
FspI TGC'GCA
Cuts at: 0 187 936
Size: 187 749
HaeII r_GCGC'y
Cuts at: 0 267 323 936
Size: 267 56 613
Fragments arranged by size:
613 267 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 275 307 936
Size: 275 32 629
Fragments arranged by size:
629 275 32
KasI G'GCGC_C
Cuts at: 0 263 319 936
Size: 263 56 617
Fragments arranged by size:
617 263 56
MslI CAynn'nnrTG
Cuts at: 0 16 133 936
Size: 16 117 803
Fragments arranged by size:
803 117 16
MspA1I CmG'CkG
Cuts at: 0 555 936
Size: 555 381
NaeI GCC'GGC
Cuts at: 0 262 936
Size: 262 674
NarI GG'CG_CC
Cuts at: 0 264 320 936
Size: 264 56 616
Fragments arranged by size:
616 264 56
NcoI C'CATG_G
Cuts at: 0 17 347 936
Size: 17 330 589
Fragments arranged by size:
589 330 17
NgoMIV G'CCGG_C
Cuts at: 0 260 936
Size: 260 676
NheI G'CTAG_C
Cuts at: 0 426 936
Size: 426 510
PstI C_TGCA'G
Cuts at: 0 554 590 936
Size: 554 36 346
Fragments arranged by size:
554 346 36
PvuII CAG'CTG
Cuts at: 0 555 936
Size: 555 381
SapI GCTCTTCn'nnn_
Cuts at: 0 82 936
Size: 82 854
SfcI C'TryA_G
Cuts at: 0 421 550 586 864 936
Size: 421 129 36 278 72
Fragments arranged by size:
421 278 129 72 36
SfoI GGC'GCC
Cuts at: 0 265 321 936
Size: 265 56 615
Fragments arranged by size:
615 265 56
StuI AGG'CCT
Cuts at: 0 706 936
Size: 706 230
StyI C'CwwG_G
Cuts at: 0 17 200 347 936
Size: 17 183 147 589
Fragments arranged by size:
589 183 147 17
TatI w'GTAC_w
Cuts at: 0 173 936
Size: 173 763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 253 810 936
Size: 253 557 126
Fragments arranged by size:
557 253 126
Enzymes that do cut:
AarI AccI BaeI BamHI BanI BanII BbeI BciVI
BclI BglI BlpI BmtI BpmI BsaI BsaHI BsaWI
BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BsrFI BstEII
BstYI Bsu36I BtgI BtsI DraIII EaeI EarI Eco57MI
FspI HaeII Hin4I KasI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI PstI PvuII SapI SfcI SfoI
StuI StyI TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BbsI BbvCI BcgI
BfrBI BglII Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaAI BsaBI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NdeI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI