Restriction map for
pFN21AE5974
(Linear) (Six-base) MAPSORT of: KIEE5974.seq Check: 3281 from: 1 to: 936
KIEE5974.orf
With 182 enzymes: SgfI *
December 5, 2014 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 936
Size: 294 642
AflIII A'CryG_T
Cuts at: 0 218 936
Size: 218 718
AlwNI CAG_nnn'CTG
Cuts at: 0 75 936
Size: 75 861
ApaLI G'TGCA_C
Cuts at: 0 324 936
Size: 324 612
ApoI r'AATT_y
Cuts at: 0 886 936
Size: 886 50
AvaI C'yCGr_G
Cuts at: 0 411 936
Size: 411 525
BanII G_rGCy'C
Cuts at: 0 193 808 936
Size: 193 615 128
Fragments arranged by size:
615 193 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 741 936
Size: 741 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 261 295 936
Size: 261 34 641
Fragments arranged by size:
641 261 34
BclI T'GATC_A
Cuts at: 0 470 936
Size: 470 466
BglI GCCn_nnn'nGGC
Cuts at: 0 352 936
Size: 352 584
BglII A'GATC_T
Cuts at: 0 209 936
Size: 209 727
Bme1580I G_kGCm'C
Cuts at: 0 328 936
Size: 328 608
BmgBI CAC'GTC
Cuts at: 0 399 936
Size: 399 537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 226 244 265 409 474 723 936
Size: 168 58 18 21 144 65 249 213
Fragments arranged by size:
249 213 168 144 65 58 21 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 936
Size: 538 398
BsaAI yAC'GTr
Cuts at: 0 219 369 936
Size: 219 150 567
Fragments arranged by size:
567 219 150
BsaHI Gr'CG_yC
Cuts at: 0 896 936
Size: 896 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 936
Size: 199 30 707
Fragments arranged by size:
707 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 247 936
Size: 76 171 689
Fragments arranged by size:
689 171 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 936
Size: 549 387
BsiEI CG_ry'CG
Cuts at: 0 363 936
Size: 363 573
BsiHKAI G_wGCw'C
Cuts at: 0 193 328 808 936
Size: 193 135 480 128
Fragments arranged by size:
480 193 135 128
Bsp1286I G_dGCh'C
Cuts at: 0 193 328 808 936
Size: 193 135 480 128
Fragments arranged by size:
480 193 135 128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 727 936
Size: 83 644 209
Fragments arranged by size:
644 209 83
BsrBI CCG'CTC
Cuts at: 0 630 936
Size: 630 306
BsrFI r'CCGG_y
Cuts at: 0 694 936
Size: 694 242
BsrGI T'GTAC_A
Cuts at: 0 830 936
Size: 830 106
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 936
Size: 75 861
BstEII G'GTnAC_C
Cuts at: 0 741 936
Size: 741 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 936
Size: 243 693
BstYI r'GATC_y
Cuts at: 0 209 936
Size: 209 727
Bsu36I CC'TnA_GG
Cuts at: 0 874 936
Size: 874 62
BtgI C'CryG_G
Cuts at: 0 53 350 737 936
Size: 53 297 387 199
Fragments arranged by size:
387 297 199 53
BtsI GCAGTG_nn'
Cuts at: 0 578 936
Size: 578 358
DraIII CAC_nnn'GTG
Cuts at: 0 332 936
Size: 332 604
EaeI y'GGCC_r
Cuts at: 0 128 371 635 689 936
Size: 128 243 264 54 247
Fragments arranged by size:
264 247 243 128 54
EarI CTCTTCn'nnn_
Cuts at: 0 85 94 936
Size: 85 9 842
Fragments arranged by size:
842 85 9
EcoICRI GAG'CTC
Cuts at: 0 191 806 936
Size: 191 615 130
Fragments arranged by size:
615 191 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 226 244 265 294 409 474 723
Size: 168 58 18 21 29 115 65 249
Cuts at: 723 936
Size: 213
Fragments arranged by size:
249 213 168 115 65 58 29 21
18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 915 936
Size: 915 21
FspI TGC'GCA
Cuts at: 0 679 936
Size: 679 257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 833 936
Size: 536 297 103
Fragments arranged by size:
536 297 103
MscI TGG'CCA
Cuts at: 0 130 373 637 936
Size: 130 243 264 299
Fragments arranged by size:
299 264 243 130
MslI CAynn'nnrTG
Cuts at: 0 40 466 619 936
Size: 40 426 153 317
Fragments arranged by size:
426 317 153 40
MspA1I CmG'CkG
Cuts at: 0 387 429 936
Size: 387 42 507
Fragments arranged by size:
507 387 42
NcoI C'CATG_G
Cuts at: 0 350 936
Size: 350 586
PasI CC'CwG_GG
Cuts at: 0 61 936
Size: 61 875
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 936
Size: 432 504
PfoI T'CCnGG_A
Cuts at: 0 203 936
Size: 203 733
PstI C_TGCA'G
Cuts at: 0 71 585 936
Size: 71 514 351
Fragments arranged by size:
514 351 71
PvuII CAG'CTG
Cuts at: 0 429 936
Size: 429 507
SacI G_AGCT'C
Cuts at: 0 193 808 936
Size: 193 615 128
Fragments arranged by size:
615 193 128
SapI GCTCTTCn'nnn_
Cuts at: 0 85 936
Size: 85 851
SfcI C'TryA_G
Cuts at: 0 67 581 936
Size: 67 514 355
Fragments arranged by size:
514 355 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 936
Size: 352 584
SmaI CCC'GGG
Cuts at: 0 413 936
Size: 413 523
SmlI C'TyrA_G
Cuts at: 0 553 936
Size: 553 383
StuI AGG'CCT
Cuts at: 0 910 936
Size: 910 26
StyI C'CwwG_G
Cuts at: 0 20 350 911 936
Size: 20 330 561 25
Fragments arranged by size:
561 330 25 20
TatI w'GTAC_w
Cuts at: 0 176 830 936
Size: 176 654 106
Fragments arranged by size:
654 176 106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 555 585 936
Size: 201 354 30 351
Fragments arranged by size:
354 351 201 30
XmaI C'CCGG_G
Cuts at: 0 411 936
Size: 411 525
XmnI GAAnn'nnTTC
Cuts at: 0 526 936
Size: 526 410
Enzymes that do cut:
AcuI AflIII AlwNI ApaLI ApoI AvaI BanII BbsI
BcgI BclI BglI BglII Bme1580I BmgBI BpmI BpuEI
BsaAI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI
FalI FspI MmeI MscI MslI MspA1I NcoI PasI
PflMI PfoI PstI PvuII SacI SapI SfcI SfiI
SmaI SmlI StuI StyI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BfrBI
BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaWI
BseYI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII
BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XhoI ZraI