Restriction map for
pFN21AE5973
(Linear) (Six-base) MAPSORT of: KIEE5973.seq Check: 8414 from: 1 to: 936
KIEE5973.orf
With 182 enzymes: SgfI *
December 5, 2014 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 164 936
Size: 164 772
AccI GT'mk_AC
Cuts at: 0 225 936
Size: 225 711
AflIII A'CryG_T
Cuts at: 0 712 936
Size: 712 224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 936
Size: 397 539
ApoI r'AATT_y
Cuts at: 0 57 186 203 571 674 936
Size: 57 129 17 368 103 262
Fragments arranged by size:
368 262 129 103 57 17
AseI AT'TA_AT
Cuts at: 0 631 936
Size: 631 305
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 378 411 936
Size: 378 33 525
Fragments arranged by size:
525 378 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 919 936
Size: 72 847 17
Fragments arranged by size:
847 72 17
BbvCI CC'TCA_GC
Cuts at: 0 780 936
Size: 780 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 395 429 936
Size: 395 34 507
Fragments arranged by size:
507 395 34
BlpI GC'TnA_GC
Cuts at: 0 103 936
Size: 103 833
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 936
Size: 122 814
Bpu10I CC'TnA_GC
Cuts at: 0 780 936
Size: 780 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 822 936
Size: 411 411 114
Fragments arranged by size:
411 411 114
BseYI C'CCAG_C
Cuts at: 0 465 936
Size: 465 471
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 936
Size: 399 537
BspHI T'CATG_A
Cuts at: 0 398 936
Size: 398 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 936
Size: 164 772
BsrGI T'GTAC_A
Cuts at: 0 767 936
Size: 767 169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 936
Size: 237 699
BstZ17I GTA'TAC
Cuts at: 0 226 936
Size: 226 710
BtsI GCAGTG_nn'
Cuts at: 0 418 541 936
Size: 418 123 395
Fragments arranged by size:
418 395 123
DraI TTT'AAA
Cuts at: 0 485 936
Size: 485 451
EaeI y'GGCC_r
Cuts at: 0 365 936
Size: 365 571
EarI CTCTTCn'nnn_
Cuts at: 0 402 936
Size: 402 534
EcoRI G'AATT_C
Cuts at: 0 674 936
Size: 674 262
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 936
Size: 781 32 123
Fragments arranged by size:
781 123 32
HindIII A'AGCT_T
Cuts at: 0 487 890 936
Size: 487 403 46
Fragments arranged by size:
487 403 46
MscI TGG'CCA
Cuts at: 0 367 936
Size: 367 569
MslI CAynn'nnrTG
Cuts at: 0 97 763 901 936
Size: 97 666 138 35
Fragments arranged by size:
666 138 97 35
MspA1I CmG'CkG
Cuts at: 0 755 936
Size: 755 181
NspI r_CATG'y
Cuts at: 0 102 716 936
Size: 102 614 220
Fragments arranged by size:
614 220 102
PciI A'CATG_T
Cuts at: 0 712 936
Size: 712 224
PstI C_TGCA'G
Cuts at: 0 161 936
Size: 161 775
PvuII CAG'CTG
Cuts at: 0 755 936
Size: 755 181
SfcI C'TryA_G
Cuts at: 0 157 498 936
Size: 157 341 438
Fragments arranged by size:
438 341 157
SmlI C'TyrA_G
Cuts at: 0 390 801 936
Size: 390 411 135
Fragments arranged by size:
411 390 135
SpeI A'CTAG_T
Cuts at: 0 685 936
Size: 685 251
SspI AAT'ATT
Cuts at: 0 510 635 936
Size: 510 125 301
Fragments arranged by size:
510 301 125
StuI AGG'CCT
Cuts at: 0 703 936
Size: 703 233
TatI w'GTAC_w
Cuts at: 0 767 936
Size: 767 169
XmnI GAAnn'nnTTC
Cuts at: 0 678 936
Size: 678 258
Enzymes that do cut:
AarI AccI AflIII AlfI ApoI AseI BaeI BbsI
BbvCI BcgI BlpI BmrI Bpu10I BpuEI BseYI BsgI
BspHI BspMI BsrGI BstXI BstZ17I BtsI DraI EaeI
EarI EcoRI Hin4I HindIII MscI MslI MspA1I NspI
PciI PstI PvuII SfcI SmlI SpeI SspI StuI
TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BciVI BclI BfrBI
BglI BglII Bme1580I BmgBI BmtI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI