(Linear) (Six-base) MAPSORT of: KIEE5969.seq  Check: 8200  from: 1  to: 936



KIEE5969.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:16  ..

ApoI r'AATT_y

Cuts at:      0     299     416     936 
   Size:        299     117     520

  Fragments arranged by size: 

                 520     299     117
 
AvaI C'yCGr_G

Cuts at:      0     512     656     936 
   Size:        512     144     280

  Fragments arranged by size: 

                 512     280     144
 
BamHI G'GATC_C

Cuts at:      0     446     936 
   Size:        446     490
 
BanII G_rGCy'C

Cuts at:      0     457     936 
   Size:        457     479
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710     936 
   Size:        710     226
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     402     515     936 
   Size:        402     113     421

  Fragments arranged by size: 

                 421     402     113
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     175     936 
   Size:        130      45     761

  Fragments arranged by size: 

                 761     130      45
 
BseYI C'CCAG_C

Cuts at:      0     158     936 
   Size:        158     778
 
BsiHKAI G_wGCw'C

Cuts at:      0     900     936 
   Size:        900      36
 
BsmI GAATG_Cn'

Cuts at:      0     337     936 
   Size:        337     599
 
Bsp1286I G_dGCh'C

Cuts at:      0     457     900     936 
   Size:        457     443      36

  Fragments arranged by size: 

                 457     443      36
 
BspHI T'CATG_A

Cuts at:      0     404     936 
   Size:        404     532
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     751     936 
   Size:        751     185
 
BsrDI GCAATG_nn'

Cuts at:      0     573     936 
   Size:        573     363
 
BsrGI T'GTAC_A

Cuts at:      0     773     936 
   Size:        773     163
 
BstEII G'GTnAC_C

Cuts at:      0     888     936 
   Size:        888      48
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     279     936 
   Size:        279     657
 
BstYI r'GATC_y

Cuts at:      0     446     685     936 
   Size:        446     239     251

  Fragments arranged by size: 

                 446     251     239
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     627     936 
   Size:        627     309
 
BtsI GCAGTG_nn'

Cuts at:      0     316     936 
   Size:        316     620
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     402     437     936 
   Size:        402      35     499

  Fragments arranged by size: 

                 499     402      35
 
EaeI y'GGCC_r

Cuts at:      0      95     936 
   Size:         95     841
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     936 
   Size:         85     851
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710     936 
   Size:        710     226
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     261     936 
   Size:        261     675
 
HincII GTy'rAC

Cuts at:      0     193     936 
   Size:        193     743
 
HindIII A'AGCT_T

Cuts at:      0     707     936 
   Size:        707     229
 
MscI TGG'CCA

Cuts at:      0      97     936 
   Size:         97     839
 
MslI CAynn'nnrTG

Cuts at:      0     172     277     679     739     847     936 
   Size:        172     105     402      60     108      89

  Fragments arranged by size: 

                 402     172     108     105      89      60
 
PfoI T'CCnGG_A

Cuts at:      0     442     936 
   Size:        442     494
 
PspXI vC'TCGA_Gb

Cuts at:      0     656     936 
   Size:        656     280
 
SfcI C'TryA_G

Cuts at:      0     479     867     936 
   Size:        479     388      69

  Fragments arranged by size: 

                 479     388      69
 
SmlI C'TyrA_G

Cuts at:      0     656     936 
   Size:        656     280
 
SspI AAT'ATT

Cuts at:      0     581     936 
   Size:        581     355
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      82     172     936 
   Size:         82      90     764

  Fragments arranged by size: 

                 764      90      82
 
TatI w'GTAC_w

Cuts at:      0     176     749     773     936 
   Size:        176     573      24     163

  Fragments arranged by size: 

                 573     176     163      24
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     105     936 
   Size:        105     831
 
XhoI C'TCGA_G

Cuts at:      0     656     936 
   Size:        656     280
 
XmnI GAAnn'nnTTC

Cuts at:      0     335     590     936 
   Size:        335     255     346

  Fragments arranged by size: 

                 346     335     255
 

 Enzymes that do cut:

     ApoI     AvaI    BamHI    BanII     BpmI     BsaI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI   BstEII
    BstXI    BstYI    BtgZI     BtsI    CspCI     EaeI     EarI  Eco57MI
     FalI   HincII  HindIII     MscI     MslI     PfoI    PspXI     SfcI
     SmlI     SspI    TaqII     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I     BtgI     ClaI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XmaI     ZraI