(Linear) (Six-base) MAPSORT of: KIEE5965.seq  Check: 1978  from: 1  to: 936



KIEE5965.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:16  ..

AccI GT'mk_AC

Cuts at:      0     221     936 
   Size:        221     715
 
AfeI AGC'GCT

Cuts at:      0     358     936 
   Size:        358     578
 
AflIII A'CryG_T

Cuts at:      0      98     712     936 
   Size:         98     614     224

  Fragments arranged by size: 

                 614     224      98
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     936 
   Size:        397     539
 
AlwNI CAG_nnn'CTG

Cuts at:      0     568     936 
   Size:        568     368
 
ApoI r'AATT_y

Cuts at:      0     186     232     325     936 
   Size:        186      46      93     611

  Fragments arranged by size: 

                 611     186      93      46
 
AseI AT'TA_AT

Cuts at:      0     583     936 
   Size:        583     353
 
BbvCI CC'TCA_GC

Cuts at:      0     780     936 
   Size:        780     156
 
BlpI GC'TnA_GC

Cuts at:      0     823     936 
   Size:        823     113
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     122     936 
   Size:        122     814
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     936 
   Size:        469     467
 
Bpu10I CC'TnA_GC

Cuts at:      0     780     936 
   Size:        780     156
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     936 
   Size:        142     794
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     487     936 
   Size:        487     449
 
BsaBI GATnn'nnATC

Cuts at:      0     152     936 
   Size:        152     784
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     521     936 
   Size:        305     216     415

  Fragments arranged by size: 

                 415     305     216
 
BspHI T'CATG_A

Cuts at:      0     398     936 
   Size:        398     538
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     413     936 
   Size:        413     523
 
DraIII CAC_nnn'GTG

Cuts at:      0     729     936 
   Size:        729     207
 
EaeI y'GGCC_r

Cuts at:      0     365     936 
   Size:        365     571
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     455     936 
   Size:        455     481
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     936 
   Size:        469     467
 
HaeII r_GCGC'y

Cuts at:      0     360     936 
   Size:        360     576
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      36      68     936 
   Size:         36      32     868

  Fragments arranged by size: 

                 868      36      32
 
MscI TGG'CCA

Cuts at:      0     367     936 
   Size:        367     569
 
MslI CAynn'nnrTG

Cuts at:      0      97     397     763     769     936 
   Size:         97     300     366       6     167

  Fragments arranged by size: 

                 366     300     167      97       6
 
MspA1I CmG'CkG

Cuts at:      0     294     755     936 
   Size:        294     461     181

  Fragments arranged by size: 

                 461     294     181
 
NspI r_CATG'y

Cuts at:      0     102     716     936 
   Size:        102     614     220

  Fragments arranged by size: 

                 614     220     102
 
PciI A'CATG_T

Cuts at:      0      98     712     936 
   Size:         98     614     224

  Fragments arranged by size: 

                 614     224      98
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     729     936 
   Size:        729     207
 
PstI C_TGCA'G

Cuts at:      0     551     936 
   Size:        551     385
 
PvuII CAG'CTG

Cuts at:      0     294     755     936 
   Size:        294     461     181

  Fragments arranged by size: 

                 461     294     181
 
SfcI C'TryA_G

Cuts at:      0     512     547     936 
   Size:        512      35     389

  Fragments arranged by size: 

                 512     389      35
 
SmlI C'TyrA_G

Cuts at:      0     157     936 
   Size:        157     779
 
SspI AAT'ATT

Cuts at:      0     654     936 
   Size:        654     282
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     180     936 
   Size:        180     756
 
XmnI GAAnn'nnTTC

Cuts at:      0     329     936 
   Size:        329     607
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     AlfI    AlwNI     ApoI     AseI    BbvCI
     BlpI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI     BsgI
    BspHI   BstAPI   DraIII     EaeI     EciI  Eco57MI    HaeII    Hin4I
     MscI     MslI   MspA1I     NspI     PciI    PflMI     PstI    PvuII
     SfcI     SmlI     SspI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI     BplI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI