Restriction map for
pFN21AE5962
(Linear) (Six-base) MAPSORT of: KIEE5962.seq Check: 6193 from: 1 to: 936
KIEE5962.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 434 936
Size: 186 248 502
Fragments arranged by size:
502 248 186
AflIII A'CryG_T
Cuts at: 0 718 816 936
Size: 718 98 120
Fragments arranged by size:
718 120 98
AleI CACnn'nnGTG
Cuts at: 0 721 936
Size: 721 215
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 587 619 936
Size: 587 32 317
Fragments arranged by size:
587 317 32
ApaI G_GGCC'C
Cuts at: 0 89 936
Size: 89 847
ApaLI G'TGCA_C
Cuts at: 0 781 936
Size: 781 155
BanII G_rGCy'C
Cuts at: 0 67 89 158 173 936
Size: 67 22 69 15 763
Fragments arranged by size:
763 69 67 22 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 236 270 936
Size: 236 34 666
Fragments arranged by size:
666 236 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 58 621 936
Size: 58 563 315
Fragments arranged by size:
563 315 58
Bme1580I G_kGCm'C
Cuts at: 0 89 785 936
Size: 89 696 151
Fragments arranged by size:
696 151 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 936
Size: 79 857
BsaI GGTCTCn'nnnn_
Cuts at: 0 777 936
Size: 777 159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 936
Size: 304 30 602
Fragments arranged by size:
602 304 30
BseYI C'CCAG_C
Cuts at: 0 157 936
Size: 157 779
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 523 936
Size: 461 62 413
Fragments arranged by size:
461 413 62
BsiHKAI G_wGCw'C
Cuts at: 0 67 407 659 785 860 936
Size: 67 340 252 126 75 76
Fragments arranged by size:
340 252 126 76 75 67
BsmI GAATG_Cn'
Cuts at: 0 28 936
Size: 28 908
Bsp1286I G_dGCh'C
Cuts at: 0 67 89 158 173 407 659 785 860
Size: 67 22 69 15 234 252 126 75
Cuts at: 860 936
Size: 76
Fragments arranged by size:
252 234 126 76 75 69 67 22
15
BsrDI GCAATG_nn'
Cuts at: 0 419 434 936
Size: 419 15 502
Fragments arranged by size:
502 419 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 936
Size: 144 792
BstXI CCAn_nnnn'nTGG
Cuts at: 0 222 936
Size: 222 714
BstYI r'GATC_y
Cuts at: 0 508 936
Size: 508 428
BtgI C'CryG_G
Cuts at: 0 221 936
Size: 221 715
BtsI GCAGTG_nn'
Cuts at: 0 229 680 936
Size: 229 451 256
Fragments arranged by size:
451 256 229
EaeI y'GGCC_r
Cuts at: 0 212 224 347 371 677 936
Size: 212 12 123 24 306 259
Fragments arranged by size:
306 259 212 123 24 12
EarI CTCTTCn'nnn_
Cuts at: 0 479 936
Size: 479 457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 886 936
Size: 886 50
EcoICRI GAG'CTC
Cuts at: 0 65 936
Size: 65 871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 186 434 936
Size: 79 107 248 502
Fragments arranged by size:
502 248 107 79
EcoO109I rG'GnC_Cy
Cuts at: 0 85 86 936
Size: 85 1 850
Fragments arranged by size:
850 85 1
EcoRV GAT'ATC
Cuts at: 0 573 936
Size: 573 363
HaeII r_GCGC'y
Cuts at: 0 881 936
Size: 881 55
HindIII A'AGCT_T
Cuts at: 0 453 936
Size: 453 483
MscI TGG'CCA
Cuts at: 0 214 226 349 373 679 936
Size: 214 12 123 24 306 257
Fragments arranged by size:
306 257 214 123 24 12
MslI CAynn'nnrTG
Cuts at: 0 220 721 936
Size: 220 501 215
Fragments arranged by size:
501 220 215
MspA1I CmG'CkG
Cuts at: 0 445 936
Size: 445 491
NcoI C'CATG_G
Cuts at: 0 221 936
Size: 221 715
NspI r_CATG'y
Cuts at: 0 722 802 820 918 936
Size: 722 80 18 98 18
Fragments arranged by size:
722 98 80 18 18
PciI A'CATG_T
Cuts at: 0 718 816 936
Size: 718 98 120
Fragments arranged by size:
718 120 98
PflMI CCAn_nnn'nTGG
Cuts at: 0 766 936
Size: 766 170
PspOMI G'GGCC_C
Cuts at: 0 85 936
Size: 85 851
PstI C_TGCA'G
Cuts at: 0 140 508 936
Size: 140 368 428
Fragments arranged by size:
428 368 140
PvuII CAG'CTG
Cuts at: 0 445 936
Size: 445 491
SacI G_AGCT'C
Cuts at: 0 67 936
Size: 67 869
SbfI CC_TGCA'GG
Cuts at: 0 140 936
Size: 140 796
SfcI C'TryA_G
Cuts at: 0 136 504 936
Size: 136 368 432
Fragments arranged by size:
432 368 136
StyI C'CwwG_G
Cuts at: 0 114 221 936
Size: 114 107 715
Fragments arranged by size:
715 114 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 557 936
Size: 557 379
TatI w'GTAC_w
Cuts at: 0 176 240 741 936
Size: 176 64 501 195
Fragments arranged by size:
501 195 176 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 113 145 304 336 936
Size: 113 32 159 32 600
Fragments arranged by size:
600 159 113 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 936
Size: 621 315
Enzymes that do cut:
AcuI AflIII AleI AloI ApaI ApaLI BanII BcgI
BciVI Bme1580I BpmI BsaI BsaXI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BstAPI BstXI BstYI BtgI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII
HindIII MscI MslI MspA1I NcoI NspI PciI PflMI
PspOMI PstI PvuII SacI SbfI SfcI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AlwNI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoRI FalI
FseI FspI FspAI Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI