Restriction map for
pFN21AE5961
(Linear) (Six-base) MAPSORT of: KIEE5961.seq Check: 6108 from: 1 to: 936
KIEE5961.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AccI GT'mk_AC
Cuts at: 0 830 936
Size: 830 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 936
Size: 805 131
AflIII A'CryG_T
Cuts at: 0 718 936
Size: 718 218
AlwNI CAG_nnn'CTG
Cuts at: 0 819 936
Size: 819 117
ApoI r'AATT_y
Cuts at: 0 31 476 606 680 936
Size: 31 445 130 74 256
Fragments arranged by size:
445 256 130 74 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 160 193 936
Size: 160 33 743
Fragments arranged by size:
743 160 33
BbvCI CC'TCA_GC
Cuts at: 0 687 936
Size: 687 249
BglII A'GATC_T
Cuts at: 0 299 936
Size: 299 637
BmgBI CAC'GTC
Cuts at: 0 528 936
Size: 528 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 43 936
Size: 36 7 893
Fragments arranged by size:
893 36 7
Bpu10I CC'TnA_GC
Cuts at: 0 687 936
Size: 687 249
BsaAI yAC'GTr
Cuts at: 0 719 936
Size: 719 217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 918 936
Size: 918 18
BseYI C'CCAG_C
Cuts at: 0 460 936
Size: 460 476
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 651 676 799 936
Size: 443 208 25 123 137
Fragments arranged by size:
443 208 137 123 25
BsiHKAI G_wGCw'C
Cuts at: 0 677 842 936
Size: 677 165 94
Fragments arranged by size:
677 165 94
BsmI GAATG_Cn'
Cuts at: 0 191 518 936
Size: 191 327 418
Fragments arranged by size:
418 327 191
Bsp1286I G_dGCh'C
Cuts at: 0 677 842 936
Size: 677 165 94
Fragments arranged by size:
677 165 94
BspHI T'CATG_A
Cuts at: 0 107 404 936
Size: 107 297 532
Fragments arranged by size:
532 297 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 936
Size: 134 802
BsrFI r'CCGG_y
Cuts at: 0 380 694 936
Size: 380 314 242
Fragments arranged by size:
380 314 242
BstAPI GCAn_nnn'nTGC
Cuts at: 0 430 936
Size: 430 506
BstYI r'GATC_y
Cuts at: 0 299 617 936
Size: 299 318 319
Fragments arranged by size:
319 318 299
BtsI GCAGTG_nn'
Cuts at: 0 830 936
Size: 830 106
DraIII CAC_nnn'GTG
Cuts at: 0 421 936
Size: 421 515
EarI CTCTTCn'nnn_
Cuts at: 0 85 784 936
Size: 85 699 152
Fragments arranged by size:
699 152 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 43 805 936
Size: 36 7 762 131
Fragments arranged by size:
762 131 36 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 360 936
Size: 360 576
EcoO109I rG'GnC_Cy
Cuts at: 0 816 936
Size: 816 120
EcoRI G'AATT_C
Cuts at: 0 31 680 936
Size: 31 649 256
Fragments arranged by size:
649 256 31
FspI TGC'GCA
Cuts at: 0 877 936
Size: 877 59
MslI CAynn'nnrTG
Cuts at: 0 112 172 775 936
Size: 112 60 603 161
Fragments arranged by size:
603 161 112 60
MspA1I CmG'CkG
Cuts at: 0 223 691 783 936
Size: 223 468 92 153
Fragments arranged by size:
468 223 153 92
NaeI GCC'GGC
Cuts at: 0 382 696 936
Size: 382 314 240
Fragments arranged by size:
382 314 240
NgoMIV G'CCGG_C
Cuts at: 0 380 694 936
Size: 380 314 242
Fragments arranged by size:
380 314 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 505 819 936
Size: 505 314 117
Fragments arranged by size:
505 314 117
PmlI CAC'GTG
Cuts at: 0 719 936
Size: 719 217
PsiI TTA'TAA
Cuts at: 0 604 936
Size: 604 332
PstI C_TGCA'G
Cuts at: 0 299 936
Size: 299 637
PvuII CAG'CTG
Cuts at: 0 223 691 783 936
Size: 223 468 92 153
Fragments arranged by size:
468 223 153 92
SapI GCTCTTCn'nnn_
Cuts at: 0 784 936
Size: 784 152
SfcI C'TryA_G
Cuts at: 0 295 867 936
Size: 295 572 69
Fragments arranged by size:
572 295 69
StyI C'CwwG_G
Cuts at: 0 318 936
Size: 318 618
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 275 936
Size: 186 89 661
Fragments arranged by size:
661 186 89
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI BaeI BbvCI BglII
BmgBI BpmI Bpu10I BsaAI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrFI BstAPI BstYI BtsI
DraIII EarI Eco57MI EcoNI EcoO109I EcoRI FspI MslI
MspA1I NaeI NgoMIV PflMI PmlI PsiI PstI PvuII
SapI SfcI StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BmtI
BplI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoRV
FalI FseI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI