Restriction map for
pFN21AE5957
(Linear) (Six-base) MAPSORT of: KIEE5957.seq Check: 2152 from: 1 to: 936
KIEE5957.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 867 936
Size: 867 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 936
Size: 178 758
AflIII A'CryG_T
Cuts at: 0 720 936
Size: 720 216
AlwNI CAG_nnn'CTG
Cuts at: 0 410 936
Size: 410 526
ApoI r'AATT_y
Cuts at: 0 186 200 936
Size: 186 14 736
Fragments arranged by size:
736 186 14
BanII G_rGCy'C
Cuts at: 0 581 936
Size: 581 355
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 936
Size: 166 770
BbvCI CC'TCA_GC
Cuts at: 0 678 936
Size: 678 258
BclI T'GATC_A
Cuts at: 0 236 936
Size: 236 700
BlpI GC'TnA_GC
Cuts at: 0 287 936
Size: 287 649
Bpu10I CC'TnA_GC
Cuts at: 0 678 936
Size: 678 258
BsaAI yAC'GTr
Cuts at: 0 95 936
Size: 95 841
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 705 936
Size: 705 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 936
Size: 302 634
BsiHKAI G_wGCw'C
Cuts at: 0 581 936
Size: 581 355
BsmI GAATG_Cn'
Cuts at: 0 242 936
Size: 242 694
Bsp1286I G_dGCh'C
Cuts at: 0 581 936
Size: 581 355
BspHI T'CATG_A
Cuts at: 0 395 581 936
Size: 395 186 355
Fragments arranged by size:
395 355 186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 718 867 936
Size: 54 664 149 69
Fragments arranged by size:
664 149 69 54
BstYI r'GATC_y
Cuts at: 0 407 936
Size: 407 529
Bsu36I CC'TnA_GG
Cuts at: 0 181 936
Size: 181 755
BtgI C'CryG_G
Cuts at: 0 473 936
Size: 473 463
DrdI GACnn_nn'nnGTC
Cuts at: 0 505 936
Size: 505 431
EaeI y'GGCC_r
Cuts at: 0 470 936
Size: 470 466
EcoICRI GAG'CTC
Cuts at: 0 579 936
Size: 579 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 936
Size: 178 758
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 221 253 399 431 936
Size: 36 32 153 32 146 32 505
Fragments arranged by size:
505 153 146 36 32 32 32
MscI TGG'CCA
Cuts at: 0 472 936
Size: 472 464
MslI CAynn'nnrTG
Cuts at: 0 760 766 936
Size: 760 6 170
Fragments arranged by size:
760 170 6
MspA1I CmG'CkG
Cuts at: 0 486 936
Size: 486 450
NcoI C'CATG_G
Cuts at: 0 473 936
Size: 473 463
NspI r_CATG'y
Cuts at: 0 724 936
Size: 724 212
PciI A'CATG_T
Cuts at: 0 720 936
Size: 720 216
PsiI TTA'TAA
Cuts at: 0 272 424 936
Size: 272 152 512
Fragments arranged by size:
512 272 152
PstI C_TGCA'G
Cuts at: 0 65 936
Size: 65 871
PvuII CAG'CTG
Cuts at: 0 486 936
Size: 486 450
SacI G_AGCT'C
Cuts at: 0 581 936
Size: 581 355
ScaI AGT'ACT
Cuts at: 0 570 936
Size: 570 366
SfcI C'TryA_G
Cuts at: 0 61 936
Size: 61 875
SnaBI TAC'GTA
Cuts at: 0 95 936
Size: 95 841
StyI C'CwwG_G
Cuts at: 0 194 309 473 936
Size: 194 115 164 463
Fragments arranged by size:
463 194 164 115
TatI w'GTAC_w
Cuts at: 0 99 568 936
Size: 99 469 368
Fragments arranged by size:
469 368 99
XbaI T'CTAG_A
Cuts at: 0 573 936
Size: 573 363
XmnI GAAnn'nnTTC
Cuts at: 0 912 936
Size: 912 24
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApoI BanII BbsI BbvCI
BclI BlpI Bpu10I BsaAI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BstYI Bsu36I BtgI DrdI EaeI
EcoICRI Eco57MI Hin4I MscI MslI MspA1I NcoI NspI
PciI PsiI PstI PvuII SacI ScaI SfcI SnaBI
StyI TatI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII EagI EarI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XcmI XhoI XmaI ZraI