Restriction map for
pFN21AE5956
(Linear) (Six-base) MAPSORT of: KIEE5956.seq Check: 6892 from: 1 to: 936
KIEE5956.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 936
Size: 665 271
AflII C'TTAA_G
Cuts at: 0 894 936
Size: 894 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 927 936
Size: 927 9
AlwNI CAG_nnn'CTG
Cuts at: 0 688 936
Size: 688 248
ApaI G_GGCC'C
Cuts at: 0 542 936
Size: 542 394
ApoI r'AATT_y
Cuts at: 0 25 161 674 936
Size: 25 136 513 262
Fragments arranged by size:
513 262 136 25
AseI AT'TA_AT
Cuts at: 0 803 936
Size: 803 133
BanI G'GyrC_C
Cuts at: 0 316 936
Size: 316 620
BanII G_rGCy'C
Cuts at: 0 152 436 542 936
Size: 152 284 106 394
Fragments arranged by size:
394 284 152 106
BlpI GC'TnA_GC
Cuts at: 0 146 936
Size: 146 790
Bme1580I G_kGCm'C
Cuts at: 0 319 542 936
Size: 319 223 394
Fragments arranged by size:
394 319 223
BmrI ACTGGGnnnn_n'
Cuts at: 0 536 936
Size: 536 400
Bpu10I CC'TnA_GC
Cuts at: 0 430 936
Size: 430 506
BsaHI Gr'CG_yC
Cuts at: 0 590 936
Size: 590 346
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 780 936
Size: 780 156
BsiHKAI G_wGCw'C
Cuts at: 0 436 936
Size: 436 500
Bsp1286I G_dGCh'C
Cuts at: 0 152 319 436 542 936
Size: 152 167 117 106 394
Fragments arranged by size:
394 167 152 117 106
BspHI T'CATG_A
Cuts at: 0 398 936
Size: 398 538
BsrDI GCAATG_nn'
Cuts at: 0 123 911 936
Size: 123 788 25
Fragments arranged by size:
788 123 25
BstAPI GCAn_nnn'nTGC
Cuts at: 0 278 413 936
Size: 278 135 523
Fragments arranged by size:
523 278 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 936
Size: 576 360
DraIII CAC_nnn'GTG
Cuts at: 0 140 936
Size: 140 796
EaeI y'GGCC_r
Cuts at: 0 690 936
Size: 690 246
EcoICRI GAG'CTC
Cuts at: 0 434 936
Size: 434 502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 936
Size: 665 271
EcoNI CCTnn'n_nnAGG
Cuts at: 0 289 936
Size: 289 647
EcoRI G'AATT_C
Cuts at: 0 25 674 936
Size: 25 649 262
Fragments arranged by size:
649 262 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 909 936
Size: 909 27
MscI TGG'CCA
Cuts at: 0 692 936
Size: 692 244
MslI CAynn'nnrTG
Cuts at: 0 343 397 607 936
Size: 343 54 210 329
Fragments arranged by size:
343 329 210 54
MspA1I CmG'CkG
Cuts at: 0 423 936
Size: 423 513
NspI r_CATG'y
Cuts at: 0 276 936
Size: 276 660
PasI CC'CwG_GG
Cuts at: 0 616 936
Size: 616 320
PflMI CCAn_nnn'nTGG
Cuts at: 0 140 499 922 936
Size: 140 359 423 14
Fragments arranged by size:
423 359 140 14
PshAI GACnn'nnGTC
Cuts at: 0 733 936
Size: 733 203
PspOMI G'GGCC_C
Cuts at: 0 538 936
Size: 538 398
PstI C_TGCA'G
Cuts at: 0 293 422 936
Size: 293 129 514
Fragments arranged by size:
514 293 129
PvuII CAG'CTG
Cuts at: 0 423 936
Size: 423 513
SacI G_AGCT'C
Cuts at: 0 436 936
Size: 436 500
SfcI C'TryA_G
Cuts at: 0 289 418 936
Size: 289 129 518
Fragments arranged by size:
518 289 129
SmlI C'TyrA_G
Cuts at: 0 894 936
Size: 894 42
StuI AGG'CCT
Cuts at: 0 59 295 936
Size: 59 236 641
Fragments arranged by size:
641 236 59
StyI C'CwwG_G
Cuts at: 0 54 936
Size: 54 882
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 429 936
Size: 429 507
TatI w'GTAC_w
Cuts at: 0 170 936
Size: 170 766
XmnI GAAnn'nnTTC
Cuts at: 0 678 936
Size: 678 258
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApaI ApoI AseI BanI
BanII BlpI Bme1580I BmrI Bpu10I BsaHI BseRI BsiHKAI
Bsp1286I BspHI BsrDI BstAPI BstXI DraIII EaeI EcoICRI
Eco57MI EcoNI EcoRI FalI MscI MslI MspA1I NspI
PasI PflMI PshAI PspOMI PstI PvuII SacI SfcI
SmlI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI
EciI EcoO109I EcoRV FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI