(Linear) (Six-base) MAPSORT of: KIEE5955.seq  Check: 182  from: 1  to: 936



KIEE5955.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     721     936 
   Size:        721     215
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665     815     911     936 
   Size:        665     150      96      25

  Fragments arranged by size: 

                 665     150      96      25
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     936 
   Size:        397     539
 
ApoI r'AATT_y

Cuts at:      0      25     203     325     673     936 
   Size:         25     178     122     348     263

  Fragments arranged by size: 

                 348     263     178     122      25
 
BclI T'GATC_A

Cuts at:      0     455     516     936 
   Size:        455      61     420

  Fragments arranged by size: 

                 455     420      61
 
BlpI GC'TnA_GC

Cuts at:      0     686     936 
   Size:        686     250
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     936 
   Size:        142     794
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     803     833     936 
   Size:        803      30     103

  Fragments arranged by size: 

                 803     103      30
 
BseYI C'CCAG_C

Cuts at:      0     152     936 
   Size:        152     784
 
BsiHKAI G_wGCw'C

Cuts at:      0     824     936 
   Size:        824     112
 
BsmI GAATG_Cn'

Cuts at:      0     224     936 
   Size:        224     712
 
Bsp1286I G_dGCh'C

Cuts at:      0     824     936 
   Size:        824     112
 
BspHI T'CATG_A

Cuts at:      0     398     936 
   Size:        398     538
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     721     936 
   Size:        721     215
 
BsrDI GCAATG_nn'

Cuts at:      0     128     936 
   Size:        128     808
 
EarI CTCTTCn'nnn_

Cuts at:      0     723     936 
   Size:        723     213
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665     815     911     936 
   Size:        665     150      96      25

  Fragments arranged by size: 

                 665     150      96      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     720     815     936 
   Size:        720      95     121

  Fragments arranged by size: 

                 720     121      95
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     224     256     440     465     472     497     936 
   Size:        224      32     184      25       7      25     439

  Fragments arranged by size: 

                 439     224     184      32      25      25       7
 
SapI GCTCTTCn'nnn_

Cuts at:      0     723     936 
   Size:        723     213
 
SmlI C'TyrA_G

Cuts at:      0     157     936 
   Size:        157     779
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     803     835     936 
   Size:        803      32     101

  Fragments arranged by size: 

                 803     101      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      29     936 
   Size:         29     907
 

 Enzymes that do cut:

     AarI     AcuI     AlfI     ApoI     BclI     BlpI    BpuEI    BsaXI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI     EarI
  Eco57MI     FalI    Hin4I     SapI     SmlI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI