Restriction map for
pFN21AE5953
(Linear) (Six-base) MAPSORT of: KIEE5953.seq Check: 9352 from: 1 to: 936
KIEE5953.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 936
Size: 721 215
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 936
Size: 554 382
AflII C'TTAA_G
Cuts at: 0 924 936
Size: 924 12
AflIII A'CryG_T
Cuts at: 0 723 936
Size: 723 213
AleI CACnn'nnGTG
Cuts at: 0 223 936
Size: 223 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 491 936
Size: 491 445
ApaLI G'TGCA_C
Cuts at: 0 503 936
Size: 503 433
AvrII C'CTAG_G
Cuts at: 0 36 936
Size: 36 900
BanII G_rGCy'C
Cuts at: 0 63 464 894 936
Size: 63 401 430 42
Fragments arranged by size:
430 401 63 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 567 936
Size: 567 369
BglII A'GATC_T
Cuts at: 0 293 936
Size: 293 643
Bme1580I G_kGCm'C
Cuts at: 0 230 507 936
Size: 230 277 429
Fragments arranged by size:
429 277 230
BmgBI CAC'GTC
Cuts at: 0 204 936
Size: 204 732
Bpu10I CC'TnA_GC
Cuts at: 0 181 484 936
Size: 181 303 452
Fragments arranged by size:
452 303 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 655 768 936
Size: 655 113 168
Fragments arranged by size:
655 168 113
BsaWI w'CCGG_w
Cuts at: 0 253 936
Size: 253 683
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 470 655 936
Size: 310 160 185 281
Fragments arranged by size:
310 281 185 160
BsiHKAI G_wGCw'C
Cuts at: 0 507 936
Size: 507 429
Bsp1286I G_dGCh'C
Cuts at: 0 63 230 464 507 894 936
Size: 63 167 234 43 387 42
Fragments arranged by size:
387 234 167 63 43 42
BspHI T'CATG_A
Cuts at: 0 77 125 398 936
Size: 77 48 273 538
Fragments arranged by size:
538 273 77 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 936
Size: 721 215
BsrFI r'CCGG_y
Cuts at: 0 152 936
Size: 152 784
BssSI C'ACGA_G
Cuts at: 0 508 936
Size: 508 428
BstBI TT'CG_AA
Cuts at: 0 578 936
Size: 578 358
BstYI r'GATC_y
Cuts at: 0 293 936
Size: 293 643
Bsu36I CC'TnA_GG
Cuts at: 0 418 936
Size: 418 518
BtgI C'CryG_G
Cuts at: 0 344 936
Size: 344 592
ClaI AT'CG_AT
Cuts at: 0 515 936
Size: 515 421
DrdI GACnn_nn'nnGTC
Cuts at: 0 571 936
Size: 571 365
EarI CTCTTCn'nnn_
Cuts at: 0 79 723 936
Size: 79 644 213
Fragments arranged by size:
644 213 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 936
Size: 554 382
EcoO109I rG'GnC_Cy
Cuts at: 0 534 936
Size: 534 402
EcoRV GAT'ATC
Cuts at: 0 388 936
Size: 388 548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 720 936
Size: 720 216
MslI CAynn'nnrTG
Cuts at: 0 166 202 223 397 936
Size: 166 36 21 174 539
Fragments arranged by size:
539 174 166 36 21
MspA1I CmG'CkG
Cuts at: 0 411 454 936
Size: 411 43 482
Fragments arranged by size:
482 411 43
NcoI C'CATG_G
Cuts at: 0 344 936
Size: 344 592
NspI r_CATG'y
Cuts at: 0 678 727 936
Size: 678 49 209
Fragments arranged by size:
678 209 49
PasI CC'CwG_GG
Cuts at: 0 312 936
Size: 312 624
PciI A'CATG_T
Cuts at: 0 723 936
Size: 723 213
PstI C_TGCA'G
Cuts at: 0 470 936
Size: 470 466
PvuII CAG'CTG
Cuts at: 0 411 454 936
Size: 411 43 482
Fragments arranged by size:
482 411 43
SapI GCTCTTCn'nnn_
Cuts at: 0 723 936
Size: 723 213
SbfI CC_TGCA'GG
Cuts at: 0 470 936
Size: 470 466
SfcI C'TryA_G
Cuts at: 0 466 683 861 936
Size: 466 217 178 75
Fragments arranged by size:
466 217 178 75
SmlI C'TyrA_G
Cuts at: 0 924 936
Size: 924 12
StuI AGG'CCT
Cuts at: 0 703 936
Size: 703 233
StyI C'CwwG_G
Cuts at: 0 36 344 936
Size: 36 308 592
Fragments arranged by size:
592 308 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 304 392 436 936
Size: 304 88 44 500
Fragments arranged by size:
500 304 88 44
TatI w'GTAC_w
Cuts at: 0 170 936
Size: 170 766
Tth111I GACn'n_nGTC
Cuts at: 0 429 936
Size: 429 507
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AlfI ApaLI AvrII
BanII BbsI BglII Bme1580I BmgBI Bpu10I BsaI BsaWI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI BssSI BstBI
BstYI Bsu36I BtgI ClaI DrdI EarI Eco57MI EcoO109I
EcoRV FalI MslI MspA1I NcoI NspI PasI PciI
PstI PvuII SapI SbfI SfcI SmlI StuI StyI
TaqII TatI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AloI
AlwNI ApaI ApoI AscI AseI AsiSI AvaI BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmrI BmtI BplI BpmI BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrGI BssHII BstAPI BstEII
BstXI BstZ17I BtgZI BtsI CspCI DraI DraIII EaeI
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI XbaI XcmI XhoI XmaI XmnI
ZraI