Restriction map for
pFN21AE5952
(Linear) (Six-base) MAPSORT of: KIEE5952.seq Check: 7051 from: 1 to: 936
KIEE5952.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AatII G_ACGT'C
Cuts at: 0 702 936
Size: 702 234
ApoI r'AATT_y
Cuts at: 0 299 416 936
Size: 299 117 520
Fragments arranged by size:
520 299 117
AseI AT'TA_AT
Cuts at: 0 901 936
Size: 901 35
AvaI C'yCGr_G
Cuts at: 0 512 656 936
Size: 512 144 280
Fragments arranged by size:
512 280 144
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 215 248 936
Size: 215 33 688
Fragments arranged by size:
688 215 33
BamHI G'GATC_C
Cuts at: 0 446 936
Size: 446 490
BanII G_rGCy'C
Cuts at: 0 457 936
Size: 457 479
BbvCI CC'TCA_GC
Cuts at: 0 187 936
Size: 187 749
BciVI GTATCCnnnnn_n'
Cuts at: 0 147 936
Size: 147 789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 936
Size: 710 226
Bpu10I CC'TnA_GC
Cuts at: 0 187 936
Size: 187 749
BsaI GGTCTCn'nnnn_
Cuts at: 0 402 515 936
Size: 402 113 421
Fragments arranged by size:
421 402 113
BsaHI Gr'CG_yC
Cuts at: 0 699 936
Size: 699 237
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 130 175 936
Size: 28 102 45 761
Fragments arranged by size:
761 102 45 28
BseYI C'CCAG_C
Cuts at: 0 158 936
Size: 158 778
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 936
Size: 207 729
BsmI GAATG_Cn'
Cuts at: 0 337 776 936
Size: 337 439 160
Fragments arranged by size:
439 337 160
Bsp1286I G_dGCh'C
Cuts at: 0 457 936
Size: 457 479
BspHI T'CATG_A
Cuts at: 0 404 936
Size: 404 532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 751 936
Size: 751 185
BsrDI GCAATG_nn'
Cuts at: 0 316 573 936
Size: 316 257 363
Fragments arranged by size:
363 316 257
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 936
Size: 279 657
BstYI r'GATC_y
Cuts at: 0 446 685 936
Size: 446 239 251
Fragments arranged by size:
446 251 239
BtsI GCAGTG_nn'
Cuts at: 0 368 936
Size: 368 568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 402 437 936
Size: 402 35 499
Fragments arranged by size:
499 402 35
EaeI y'GGCC_r
Cuts at: 0 95 936
Size: 95 841
EarI CTCTTCn'nnn_
Cuts at: 0 85 581 936
Size: 85 496 355
Fragments arranged by size:
496 355 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 936
Size: 710 226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 261 936
Size: 261 675
HindIII A'AGCT_T
Cuts at: 0 707 936
Size: 707 229
MscI TGG'CCA
Cuts at: 0 97 936
Size: 97 839
MslI CAynn'nnrTG
Cuts at: 0 172 277 679 739 847 936
Size: 172 105 402 60 108 89
Fragments arranged by size:
402 172 108 105 89 60
PfoI T'CCnGG_A
Cuts at: 0 442 936
Size: 442 494
PspXI vC'TCGA_Gb
Cuts at: 0 656 936
Size: 656 280
SfcI C'TryA_G
Cuts at: 0 458 479 936
Size: 458 21 457
Fragments arranged by size:
458 457 21
SmlI C'TyrA_G
Cuts at: 0 656 936
Size: 656 280
SspI AAT'ATT
Cuts at: 0 581 936
Size: 581 355
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 936
Size: 82 854
TatI w'GTAC_w
Cuts at: 0 176 749 936
Size: 176 573 187
Fragments arranged by size:
573 187 176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 105 936
Size: 105 831
XhoI C'TCGA_G
Cuts at: 0 656 936
Size: 656 280
XmnI GAAnn'nnTTC
Cuts at: 0 335 936
Size: 335 601
ZraI GAC'GTC
Cuts at: 0 700 936
Size: 700 236
Enzymes that do cut:
AatII ApoI AseI AvaI BaeI BamHI BanII BbvCI
BciVI BpmI Bpu10I BsaI BsaHI BseRI BseYI BsgI
BsmI Bsp1286I BspHI BspMI BsrDI BstXI BstYI BtsI
CspCI EaeI EarI Eco57MI FalI HindIII MscI MslI
PfoI PspXI SfcI SmlI SspI TaqII TatI XcmI
XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AsiSI AvrII BanI BbeI BbsI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI StyI SwaI TstI Tth111I XbaI
XmaI